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EC number: 272-782-3 | CAS number: 68911-68-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 February 2018 to 17 October 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Description: Dark amber viscous liquid
- CAS Number: 68911-68-2
- Purity: 99%, this substance has an Unknown or Variable composition, is a Complex reaction product, or a Biological material (UVCB) - Analytical monitoring:
- yes
- Details on sampling:
- Aqueous samples (10 mL) were collected from the control and each surviving test concentration at 0 (fresh media, each replicate) and 24 and 48 hours (old media, pooled replicates) for analysis. The aqueous samples were diluted with acetonitrile, then diluted further with acetonitrile/Elendt M4 medium (1:1, v:v), 4:1, as required to bring the response within the calibration range. Duplicate samples were collected, one for chemical analysis and one as a ‘back-up’ should further analysis be required.
- Vehicle:
- no
- Details on test solutions:
- Preliminary analysis in another study with this UVCB test substance indicated that the test substance was soluble in test media. Test solution preparation followed the method developed in that study. At the start of the test, test substance was weighed onto a glass slide and placed in Elendt M4 medium. The preparation was stirred for ca 23 hours at a rate such that a slight dimple was formed at the surface. After the stirring period, the preparation was allowed to settle for ca 1 hour after which the aqueous phase was removed by careful mid-depth siphoning to give the 10 mg/L test concentration. The stock solutions were diluted with Elendt M4 media to give the remaining test concentrations. The control solution consisted only of Elendt M4 medium.
The test preparations were observed to be colourless solutions throughout the duration of the test. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Test organism: Juvenile Daphnia magna (Straus), less than 24 hours old
- Source: Parental laboratory cultures, held and maintained at the test facility (culture was initiated from ephippia obtained from MicroBio Tests Inc., Belgium)
- Age of parental stock: Less than 24 hours old
- Food type: Cultures fed daily with a concentrated suspension of Chlorella vulgaris
- Feeding during test: None - Test type:
- semi-static
- Water media type:
- other: Elendt M4 medium
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- Not specified.
- Test temperature:
- 20 +/- 2 ºC
- pH:
- 7.59 - 8.02
- Dissolved oxygen:
- 8.54 - 9.54 mg/L (92-102% air saturation value)
- Salinity:
- Not applicable
- Conductivity:
- Not specified.
- Nominal and measured concentrations:
- Nominal: 0 (control), 0.10, 0.32, 1.0, 3.2 and 10 mg/L
Mean Measured:- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL (nominal) glass, tall form beakers covered with clear lids
- Test solution volume: 50 mL (nominal)
- Aeration: Not aerated during the test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): Not applicable
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium prepared from trace element stock solutions and reverse-osmosis (RO) water in accordance with OECD Guideline No. 202.
OTHER TEST CONDITIONS
- Intervals of water quality measurement: The pH, dissolved oxygen and temperature were determined in freshly prepared test media at the start of the test and at media renewal at 24 hours and in the old media (pooled replicates) at 24 and 48 hours. Continuous temperatures were measured using a digital (min/max) thermometer in an additional vessel maintained in the test area.
- Adjustment of pH: None
- Photoperiod: 16-hour light : 8-hour dark
- Light intensity: Not specified
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
Statistical analysis was performed using CETIS program V 1.8.6.8. The EC50 values were calculated using linear interpolation. The no observed effect concentration (NOEC) and the lowest observed effect concentration (LOEC) were estimated by using Dunnet Multiple Comparison Test.
RANGE-FINDING STUDY
- Nominal test concentrations: Control, 0.010, 0.10, 1.0, 10 and 100 mg/L under semi-static test conditions (duplicate vessels for each)
- Results used to determine the conditions for the definitive study: The definitive test concentrations were based on the results of the range-finding test, which suggested that the 48-hour EC50 value would be between 0.1 and 10 mg/L.- Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.2 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- mobility
- Remarks on result:
- other:
- Remarks:
- (95% confidence limits, 1.5 - 3.6)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.6 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- mobility
- Remarks on result:
- other:
- Remarks:
- (95% confidence limits, 4.0 - 6.9 mg/L)
- Duration:
- 24 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 2.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- mobility
- Details on results:
- All daphnids were observed to be submerged in the test solution at 24 and 48 hours.
- Reported statistics and error estimates:
- Statistical analysis was performed using the CETIS program v 1.8.6.8. The EC50 values were calculated using linear interpolation. The no observed effect concentration (NOEC) and the lowest observed effect concentration (LOEC) were estimated empirically by observation of the data.
Immobilization in definitive test
Mean Measured Concentration (mg/L)
Number of
Daphnia magna
exposed% Immobility
at 24 hours% Immobility
at 48 hoursControl
20
-
-
0.051
20
5
10
0.19
20
5
15
0.80
20
10
10
2.8
20
15
60
8.0
20
70
100
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of the test item to the freshwater invertebrate Daphnia magna was investigated under semi-static conditions and the 48-hour EC50 value was determined to be 2.2 mg/L and the No Observed Effect Concentration (NOEC) was determined to be 0.8 mg/L, based on mean measured concentrations.
- Executive summary:
The study was conducted to determine the 48-hour acute toxicity of the test substance against the mobility of the freshwater planktonic crustacean, Daphnia magna according to OECD Guideline No. 202, under GLP conditions. The definitive test was conducted at nominal concentrations of 0.10, 0.32, 1.0, 3.2 and 10 mg/L under semi-static test conditions, with a concurrent control group. At the start of the test, five juvenile Daphnia magna were added to each test vessel. The Daphnia magna in each test vessel were observed for mobility at 24 and 48 hours. The Daphnia magna were not fed during the test.
Test solutions were analysed at 0 and 48 hours using high performance liquid chromatography (HPLC) with time of flight mass spectrometry (TOF) based on quantitation of phosphoric acid dimethyl ester constituent, the marker constituent with the most singularly abundant peak.
Analysis of the freshly prepared test media at 0 hours resulted in measured concentrations ranging from 81% to 131% of nominal. Analysis of the old media at 48 hours showed measured concentrations to range from 21% to 57% of nominal value. Given that some of the measured concentrations were outside the 80% to 120% acceptance criteria, and that no clear concentration reduction was observed over the 48-hour period, the mean measured concentrations were calculated and used for effect concentration determination. These were calculated to be 0.051, 0.19, 0.80, 2.8 and 8.0 mg/L (equivalent to 51%, 59%, 80%, 88% and 80% of nominal, respectively).
The 48-hour EC50 value was calculated to be 2.2 mg/L. The corresponding NOEC was considered to be 0.8 mg/L.
Reference
Description of key information
In an OECD Guideline 202 study, conducted according to GLP, the acute toxicity of the test item to the freshwater invertebrate, Daphnia magna, was investigated under semi-static conditions and the 48-hour EC50 value was determined to be 2.2 mg/L and the No Observed Effect Concentration (NOEC) was determined to be 0.8 mg/L, based on mean measured concentrations(Smithers Viscient (ESG) Ltd., 2018).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 2.2 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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