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Diss Factsheets
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EC number: 424-650-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/L (nominal)
- Sampling method: Sample taken at t=0, 24h (before renewal), 24h (after renewal), 96h - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetonitrile
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): 0.1 mL/L - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: Amazonas Commercial Aquarium, Shanghai
- Age at study initiation (mean and range, SD): 3 months
- Length at study initiation (mean and SD): 2.1 +/- 0.2 cm
- Weight at study initiation (mean and SD): 0.08 +/- 0.03 g
ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): yes
- Feeding frequency during acclimation: fed everyday
- Health during acclimation (any mortality observed): not subnormal growth, no mortality
FEEDING DURING TEST
- Frequency: none - not fed during test - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 180 mg/L
- Test temperature:
- 23°C +/- 1°C
- pH:
- 7.78 - 8.3.1
- Dissolved oxygen:
- >70%
- Nominal and measured concentrations:
- mean measured concentrations
- Details on test conditions:
- TEST SYSTEM
- Material, size, headspace, fill volume: 3L vessel
- Renewal rate of test solution (frequency/flow rate): once after 24 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
OTHER TEST CONDITIONS
- Photoperiod: 12 hours light, 12 hours dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Mortality and behaviour (after 3, 6, 24, 48, 72, 96 hours) - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1.6 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: limit of aqueous solubility
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: loading rate
- Details on results:
- - Behavioural abnormalities:all fish in test concentration were observed to be normal. One fish in each replicate experienced swimming disorders. No mortality was reported
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Mortality: yes
- LC50: 24h LC50 225 mg/L
- Other: expected 24h LC50 should between 200-400 mg/L - Sublethal observations / clinical signs:
The test substance remained stable throughtout the study confirmed by analytical determination.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96h LC50 value was determined to be >100mg/L based on nominal concentrations and >1.6 mg/l based on mean measured concentration
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
- Sampling method: Samples from the control, lowest, middle and highest concentrations were taken at t=24 (newly prepared media) and at t=48h from the same (spent media). Samples of ca. 100 mL were taken in brown glass bottles.
- Sample storage conditions before analysis: samples were analysed immediately after collection - Vehicle:
- no
- Details on test solutions:
- Identity and concentration of auxiliary solvent for dispersal: No solvent was employed. Test medium with 100 mg/l of the
test substance was stirred for 20 hours and subsequently
left for 4 hours. The saturated solution was drawn off and
used as such or diluted with medium. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Length at study initiation (length definition, mean, range and SD): 2.25 +/- 0.19 cm
- Weight at study initiation (mean and range, SD): 0.096 +/- 0.027g - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- ca. 210 CaCO3 210 mg/L
- Test temperature:
- 25 +/- 1C
- pH:
- 8.0 - 8.3
- Nominal and measured concentrations:
- Measured
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Beaker
- Material, size, headspace, fill volume: Glass, 2L, yes, 1.5L
- Aeration: yes
- Renewal rate of test solution (frequency/flow rate): 24h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Ground water from Linschoten (The Netherlands) - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 8.12 mg/L
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 8.12 mg/L
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 8.12 mg/L
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 8.12 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: limit of solubility
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 8.12 mg/L
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.55 mg/L
- Details on results:
- % Concentration loss over test: 11 ... 29
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96h LC50 value was determined as >8.12 mg/L. This concentration was the limit of aqueous solubility. No mortality was observed in the test.
Referenceopen allclose all
Description of key information
Two GLP-compliant, OECD guideline study conducted with Danio rerio indicate that no mortality or abnormal behaviour was seen up to the limit of aqueous solubility with 48h LC50 reported as >1.6 and >8.12 mg/l, respectively.
Key value for chemical safety assessment
Additional information
It is not appropriate to include an LC50 value for the chemical safety assessment above as this is determined to be greater than the limit of aqueous solubility.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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