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EC number: 220-605-5 | CAS number: 2834-84-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Qualifier:
- according to guideline
- Guideline:
- ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static Method)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Guanidinium phosphate (1:1)
- EC Number:
- 226-551-9
- EC Name:
- Guanidinium phosphate (1:1)
- Cas Number:
- 5423-22-3
- Molecular formula:
- CH5N3.H3O4P
- IUPAC Name:
- Guanidium phosphate (1:1)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Blank control and 100 mg/L
- Sampling
Frequency: at t=0 h and t=96 h
Volume: 1 mL
- Sample storage conditions before analysis: Samples were transported on dry ice to TNO Triskelion (P.O. Box 844, 3700 AV, Zeist, The Netherlands) and were stored in a freezer until analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Preparation of test solutions started with a nominal concentration of 100 mg/L applying a short period of vigorous shaking to accelerate the dissolving of the test substance in the test medium. The lower test concentrations for the range-finding test were prepared by subsequent dilutions of the 100 mg/L concentration in test medium.
- Controls: Blank control containing test medium without test substance or other additives
- Evidence of undissolved material (e.g. precipitate, surface film, etc): The final test solutions were all clear and colourless.
Test organisms
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: Carp
- Source: Zodiac, proefacc, "De Haar Vissen", Wageningen University and Research Centre, the Netherlands
- Length at study initiation (mean and SD): 2.3±0.3 cm (final test)
- Weight at study initiation (mean and SD): 0.29±0.10 g (final test)
- Feeding during test: None
ACCLIMATION
- Acclimation period: At least 12 days after delivery
- Acclimation conditions (same as test or not): Yes
- Feeding frequency and type of food: Daily with pelleted fish food
- Health during acclimation (any mortality observed): In the batch of fish used for the test, mortality during the seven days prior to the start of the test was less than 5%.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 180 mg CaCO3 per litre
- Test temperature:
- Final test: 21.4 - 22.5°C
- pH:
- Final test
Blank control: 7.5-7.7
100 mg/L: 6.7-7.3 - Dissolved oxygen:
- Final test (O2 (mg/L)
Blank control: 6.6 - 9.2
100 mg/L: 7.2 - 9.2 - Nominal and measured concentrations:
- Nominal (final test): 0 (blank control); 100 mg/L.
Measured (final test)
t=0h: <0.08 mg/L* (blank control) and 85 mg/L (100 mg/L)
t=96h: <0.08 mg/L* (blank control) and 97 mg/L (100 mg/L)
*) LOD was 0.08 mg/L (lowest calibration point). - Details on test conditions:
- TEST SYSTEM
- Test vessel: 10 litres, all-glass, containing 9 litres of test solution
- Aeration: None
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.23 g fish per litre
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap-water purified by reverse osmosis
- Culture medium different from test medium: No (adjusted ISO medium). However, the pH of culture medium was 7.9. Therefore fish to be exposed to the 100 mg/L concentration were acclimatized to a pH of 7.0 before the start of the test.
- Intervals of water quality measurement (dissolved oxygen content, pH and temperature): Daily in all vessels, beginning at the start of the test (day 0).
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light: 8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): At 3h:15min, 24, 48, 72 and 100 h following the start of exposure. In addition, every afternoon from day 0 and every morning from day 1 to observe for any dead or severely distressed fish.
TEST CONCENTRATIONS
- Spacing factor for test concentrations (final test): Limit test (100 mg/L)
- Range finding study
- Test concentrations: blank control, 0.1, 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- Pentachlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 33 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: guanidine content (32.7% w/w)
- Basis for effect:
- mortality (fish)
- Details on results:
- - Measured concentrations: The measured concentration was in agreement with nominal and remained stable during the test period (85-97%). Given this result the LC50-value was based on the nominal test concentration.
- Biological results: No mortality or clinical effects were observed during the test period.
- Acceptability of the test:
1. No mortality was observed in the control at the end of the test.
2. Test conditions were maintained constant throughout the test.
3. The dissolved oxygen concentration has been at least 60% of the air saturation value throughout the test (>5 mg/L at 22°C).
4. The concentrations of the substance being tested have been at least 80% of the nominal concentration throughout the test. - Results with reference substance (positive control):
- - Pentachlorophenol: The 96h-LC50 for carp was 0.17 mg/L (95% confidence interval: 0.15 and 0.27 mg/L).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96h-LC50 for carp exposed to Guanidine phosphate (1:1) was >100 mg/L.
- Executive summary:
In a 96-h acute toxicity study conducted according to OECD guideline 203, carp (Cyprinus carpio) were exposed to Guanidine phosphate (1:1) under static conditions at the following nominal concentrations (limit test): 0 (blank control) and 100 mg/L. Test substance induced no visible or lethal effects in carp at a nominal exposure concentration of 100 mg/L. The 96h-LC50 was >100 mg/L (corresponding to >33 mg guanidine/L) based on analytically confirmed nominal concentrations. The study is considered to be reliable without restrictions.
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