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EC number: 610-623-5 | CAS number: 511540-64-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 Feb - 24 May 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- reconstituted water
- Details on test solutions:
- Before exposure of daphnia, the test medium (reconstituted water and test material) was freshly prepared: The calibrated flask with test material and vehicle (reconstituted water) was treated in an ultrasonic device for 1 hour. Subsequently, the preparation was aerated, and stirred with an magnetic stirrer for further 23 hours. After 24 hours the formulation was given through a single use syringe filter (pore size 0.2 µm). The filtrate was used for the study.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: Institute of Toxicology, Merck KGaA
- Age: not older than 24 hours
FEEDING DURING TEST
- Food type: fresh water algae
- Frequency: once per week - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- ca. 14° dH (about 250 mg/L CaCO3)
- Test temperature:
- 21-22 °C
- pH:
- control group: 7.76 - 7.82
test material group: 7.27 - 7.73 - Dissolved oxygen:
- control group: 87.3 - 89.9 %
test material group: 83.2 - 88.4 % - Details on test conditions:
- Administration:
At the start of the experimental phase, 5 Daphnia magna were placed into test vessels with 10 mL of reconstituted water (4 vessels / control group) or test medium (4 vessels / test material group). The Daphnia magna were not fed, and the control medium and test medium were not aerated during the test.
Observation schedule:
The mortality was determined visually and recorded after 24 and 48 hours. Those animals which were not able to swim within 15 seconds after gentle agitation of the test container were considered to be immobile.
Laboratory tests:
The pH values and dissolved oxygen concentration (O2) were measured in the control and in test material medium at the beginning and at the end of the experimental part.
During the experimental part, the temperature was registered in a control vessel with an electronic therometer containing a maximum and minimum memory display. - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- mobility
- Details on results:
- A nominal dose of 100 mg/L (water solubility <0.01 mg/L revealed no aquatic toxicity in this test system. No remarkable observations were made concerning the appearance of the solution of the test material preparation. The test medium was a clear preparation and stayed unchanged throughout the study.
Under the given experimental conditions, the test material showed a 24 h and 48 h EC50 value >100 mg/L nominally.
- Mortality of control: no - Validity criteria fulfilled:
- yes
- Conclusions:
- A nominal dose of 100 mg/L (water solubility < 0.01 mg/L) revealed no aquatic toxicity in this test system. Under the given experimental conditions, the test material showed a 24 h and 48 h EC50 value >100 mg/L nominally.
- Executive summary:
The objective of this study was to determine the acute toxicity of the test material using Daphnia magna. For this purpose, juvenile Daphnia magna were exposed to an aqueous test material solution over 48 hours, under defined conditions. The study comprised of four test vessels per concentration containing five Daphnia magna each, i.e. 20 Daphnia magna per concentration (test medium group).
Daphnia magna were exposed to a nominal test material concentration of 100 mg/L (limit test) in an open static system.
Daphnia magna exposed to an aqueous preparation of the nominal concentration of 100 mg/L test item were not affected. The test material concentration in the aqueous medium was not quantified at the start and the end of this study due to the low water solubility (< 0.01 mg/L) and the fact that no toxicity was observed up to the limit of water solubility (< 0.01 mg/L). The 48 h EC50 value for Daphnia magna exceeded the maximum solubility (< 0.01 mg/L) of the test material in reconstituted water and, thus, could not be determined in this test.
Reference
Description of key information
OECD 202: 48-h EC50 > 100 mg/L (nominal)
Key value for chemical safety assessment
Additional information
The objective of this study was to determine the acute toxicity of the test material using Daphnia magna. For this purpose, juvenile Daphnia magna were exposed to an aqueous test material solution over 48 hours, under defined conditions. The study comprised of four test vessels per concentration containing five Daphnia magna each, i.e. 20 Daphnia magna per concentration (test medium group).
Daphnia magna were exposed to a nominal test material concentration of 100 mg/L (limit test) in an open static system.
Daphnia magna exposed to an aqueous preparation of the nominal concentration of 100 mg/L test item were not affected. The test material concentration in the aqueous medium was not quantified at the start and the end of this study due to the low water solubility (< 0.01 mg/L) and the fact that no toxicity was observed up to the limit of water solubility (< 0.01 mg/L). The 48 h EC50 value for Daphnia magna exceeded the maximum solubility (< 0.01 mg/L) of the test material in reconstituted water and, thus, could not be determined in this test.
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