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EC number: 221-297-5 | CAS number: 3058-38-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Remarks:
- purity of test substance; acclimation period, details on test animals and housing/feeding conditions not reported; eyes were washed
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 976
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- yes
- Remarks:
- purity of test substance; acclimation period, details on test animals and housing/feeding conditions not reported; eyes were washed
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- no
- Remarks:
- pre-GLP
Test material
- Reference substance name:
- 2,4,6-trinitrobenzene-1,3,5-triamine
- EC Number:
- 221-297-5
- EC Name:
- 2,4,6-trinitrobenzene-1,3,5-triamine
- Cas Number:
- 3058-38-6
- Molecular formula:
- C6H6N6O6
- IUPAC Name:
- trinitrobenzene-1,3,5-triamine
- Test material form:
- not specified
- Details on test material:
- - Name of test material as cited in the report: TATB ; 1,3,5-triamino-2,4,6-trinitrobenzene
- Lot/batch No.of test material: B-318
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: untreated right eye served as control
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 g - Duration of treatment / exposure:
- Test substance was washed from the eye of six rabbits (30 seconds after instillation in three rabbits; 5 minutes after instillation in another three rabbits); and no washing was done in three rabbits.
- Observation period (in vivo):
- 1, 4, 24, 48, 72, 96 hours and 7 days after instillation of test substance
- Number of animals or in vitro replicates:
- 9 animals
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): Test substance was washed from the eye of six rabbits (30 seconds after instillation in three rabbits; 5 minutes after instillation in another three rabbits); and no washing was done in three rabbits.
SCORING SYSTEM: Draize method
Results and discussion
In vivo
Resultsopen allclose all
- Remarks on result:
- other: See "Any other information on results incl. tables"
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Remarks on result:
- not determinable
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Remarks on result:
- not determinable
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Remarks on result:
- not determinable
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Remarks on result:
- not determinable
- Irritant / corrosive response data:
- Irritation was observed in conjunctival and iris tissues only. All three rabbits in the no-wash and 5 minute wash groups showed circumcorneal injection around the iris at the 1-hour reading. This condition persisted for 3 days in the no-wash group. Occasional circumcorneal injection responses were noted in individual rabbits at all treatment levels, but all effects involving the iris had disappeared by day 4 after treatment.
Conjunctival responses were observed at all treatment levels and generally involved mild redness, chemosis, and discharge. The treated eye of all rabbits in the 30-second wash group had returned to normal within 24 hours, whereas the treated eye of rabbits in the 5-minute wash group did not completely clear until day 4, and the no-wash group was not judged normal in response until day 7. Overall, the degree of eye irritation caused by test substance was mild but transitory.
Any other information on results incl. tables
Table 7.3.2/1: Summary of eye irritation responses in rabbits treated with test substance (three rabbits per wash condition)
Tissues graded* |
Average Irritancy Score |
||||||
Hours |
Days |
||||||
1 |
4 |
1 |
2 |
3 |
4 |
7 |
|
30 Seconds washing |
|||||||
Cornea |
0 |
0 |
0 |
0 |
0 |
|
|
Iris |
0 |
1.7 |
0 |
0 |
0 |
|
|
Conjunctivae |
5.3 |
4.0 |
0 |
0 |
0 |
|
|
5 minutes washing |
|||||||
Cornea |
0 |
0 |
0 |
0 |
0 |
|
|
Iris |
5.0 |
0 |
0 |
1.7 |
0 |
|
|
Conjunctivae |
1.7 |
2.7 |
2.0 |
2.0 |
0.7 |
0 |
|
No wash |
|||||||
Cornea |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Iris |
5.0 |
1.7 |
1.7 |
1.7 |
1.7 |
0 |
0 |
Conjunctivae |
4.0 |
2.7 |
1.3 |
0.7 |
0.7 |
0.7 |
0 |
*Maximum corneal response: 80; Maximum iris response: 10; Maximum conjunctivae response: 20
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Under the test conditions, the test substance was mild but transitory irritant to the eyes of rabbits.
- Executive summary:
In an eye irritation study, 0.1 g of test substance was instilled into the left eye of 9 New Zealand White rabbits while the right eye remained untreated and served as control. Test substance was washed from the eye of six rabbits (30 seconds after instillation in three rabbits; 5 minutes after instillation in another three rabbits); and no washing was done in three rabbits. Animals were observed at 1, 4, 24, 48, 72, 96 h and 7 days after instillation of test substance.
Irritation was observed in conjunctival and iris tissues only. All three rabbits in the no-wash and 5 minute wash groups showed circumcorneal injection around the iris at the 1-hour reading. This condition persisted for 3 days in the no-wash group. Occasional circumcorneal injection responses were noted in individual rabbits at all treatment levels, but all effects involving the iris had disappeared by day 4 after treatment. Conjunctival responses were observed at all treatment levels and generally involved mild redness, chemosis, and discharge. The treated eye of all rabbits in the 30-second wash group had returned to normal within 24 hours, whereas the treated eye of rabbits in the 5-minute wash group did not completely clear until day 4, and the no-wash group was not judged normal in response until day 7. Overall, the degree of eye irritation caused by test substance was mild but transitory.
Under the test conditions, the test substance was mild but transitory irritant to the eyes of rabbits.
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