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Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
in vivo insect germ cell study: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
In vivo genotoxicity assessment of titanium, zirconium and aluminium nanoparticles, and their microparticulated forms, in Drosophila
Author:
Demir, E et al
Year:
2013
Bibliographic source:
Chemosphere Volume 93, Issue 10, November 2013, Pages 2304-2310

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method B.20 (Sex-linked Recessive Lethal Test in Drosophila melanogaster)
GLP compliance:
not specified
Type of assay:
Drosophila SLRL assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Zirconium dioxide
EC Number:
215-227-2
EC Name:
Zirconium dioxide
Cas Number:
1314-23-4
Molecular formula:
O2Zr
IUPAC Name:
Zirconium dioxide

Test animals

Species:
Drosophila melanogaster
Details on species / strain selection:
Two strains : multiple wing hairs strain with the genetic constitution y; mwh j; and the flare-3 strain with the genetic constitution, flr3/In (3LR) TM3, Bds.
Sex:
male/female

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
Prepared in distilled water
Details on exposure:
Microparticulated 0.1, 1, 5 and 10 mM in feed
Duration of treatment / exposure:
Three day old larvea until pupation
Doses / concentrationsopen allclose all
Dose / conc.:
0.1 other: mM
Dose / conc.:
1 other: mM
Dose / conc.:
5 other: mM
Dose / conc.:
10 other: mM
No. of animals per sex per dose:
Between 10 and 20
Control animals:
yes, concurrent vehicle
other: Positive control ethyl methanesulfonate (EMS)
Positive control(s):
Ethyl methanesulfonate (EMS)

Examinations

Tissues and cell types examined:
Wing spot
Statistics:
Conditional binomial test

Results and discussion

Test results
Sex:
male/female
Genotoxicity:
negative
Toxicity:
no effects
Vehicle controls validity:
valid
Negative controls validity:
not applicable
Positive controls validity:
valid

Applicant's summary and conclusion

Conclusions:
Although not following normal guidelines, the outcome of the study considering metal salts is considered valid.

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