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Diss Factsheets
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EC number: 285-480-1 | CAS number: 85099-25-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1987
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test procedure following national standard guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: German standard test guideline, DIN 38 412 L15, 1982. Comparable to OECD 203.
- GLP compliance:
- no
Test material
- Details on test material:
- - Name of test material (as cited in study report): Dimethylcyclohexylamine- Purity: 99.9 %- Lot/batch No.: II/87
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):Not applicable
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- No data provided
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: The test solution was prepared by adding the test item to the test media without any pre-treatment. Subsequently the fish were placed into the test aquaria
Test organisms
- Test organisms (species):
- Leuciscus idus
- Details on test organisms:
- TEST ORGANISM- Common name: Golden orfe- Strain: : Leuciscus idus L. (golden variety)- Source: Fish farm Paul Eggers, 2354 Hohenwestedt, Germany- Length at study initiation (length definition, mean, range and SD): 5.3 cm (range 4.7 - 5.7 cm)- Weight at study initiation (mean and range, SD): 3.7 g (range 2.1 - 4.9 g)- Feeding during test: no - Frequency: Withdrawal of food before exposure: 1 dayACCLIMATION- Acclimation period: 4 weeks - Acclimation conditions (same as test or not): oil-free aerated and charcoal filtered tap water, flow-through systemWater temperature: 20 °C Duration of housing: about 4 weeksAcclimation to test water and test temperature: 3 days- Type and amount of food: : Growing feed F/B 50, - Feeding frequency: ad libitum.- Health during acclimation (any mortality observed):Mortality during the last 2 weeks of housing: 0.0 %Mortality during the acclimation period: 0 %Medical treatment: twice with 0.05 mg/l malachite green chloride, once with 10 mg/l tetracycline hydrochloride
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Not applicable
Test conditions
- Hardness:
- 2.5 mmol/L
- Test temperature:
- 19 - 20 °C
- pH:
- about 8.0
- Dissolved oxygen:
- 8.5 ± 0.5 mg/L
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- nominal: 10.0, 21.5, 46.4, 100 mg/L. Additionally: 100 mg/L pH adjusted
- Details on test conditions:
- TEST SYSTEM- Test vessel: - Type (delete if not applicable): aquaria open - Material, size, fill volume: glass, 30 x 22 x 24 cm, 10 L - Aeration: aeration with oil-free air- No. of organisms per vessel: 10- No. of vessels per concentration (replicates): 1- No. of vessels per control (replicates): 1- Biomass loading rate: 3.7 g fish /LTEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, part 11- Ca/Mg ratio: 4 : 1- Intervals of water quality measurement: 1, 24, 48, 72, and 96 hoursOTHER TEST CONDITIONS- Adjustment of pH: one test item concentration of 100 mg/L was pH adjusted- Photoperiod: 16:8 hour light:dark- Aeration: slight EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, symptomsTEST CONCENTRATIONS- Spacing factor for test concentrations: 2.2
- Reference substance (positive control):
- yes
- Remarks:
- Chloroacetamide
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 21.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- not neutralized
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 21.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- not neutralized
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 31.58 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- not neutralised
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: geometrical mean
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 46.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- not neutralized
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Neutralized, after pH adjustment
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: after pH-adjustment
- Details on results:
- There were no abnormalities.
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes- LC50: 26 mg/L after 48 h
- Reported statistics and error estimates:
- Observation of the results.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
- Effects may be due to the pH of the substance: no effects were observed after 96 -h when exposed to a neutralized sample (100 mg/L)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Groups of ten Golden orfe (Leuciscus idus L., Golden variety) were exposed under static conditions to DMCHA, for a period of 96 -hours. The 96 -hour LC50 was observed to be > 21.5 mg/L and < 46.4 mg/L, the corresponding no-observed effect concentration (NOEC) was 21.5 mg/L. If the results are based on the pH adjusted medium the 96 hour LC would be >100 mg/L and the corresponding NOEC would be 100 mg/L.
- Executive summary:
Groups of ten Golden orfe (Leuciscus idus L., Golden variety) were exposed under static conditions to cyclohexyldimethylamine (DMCHA), for a period of 96 -hours according to the German Standard Test Guideline, DIN 38 412 L15, 1982 which is comparable to OECD 203. Nominal exposure concentrations were control, 0.0, 21.5, 46.4, 100 mg/L, additionally: 100 mg/L pH adjusted. No analytical verification was performed therefore the results of the study were based on nominal exposure concentrations. After 96 hours 100% mortality was observed in the 46.4 and 100 (no pH adjustment) mg/L treatments, there was no mortality in any of the other treatments or control.
The toxic effect seen in the 46.4 and 100 mg/L (no pH adjustment) was due to the high pH levels in the test media. There was no mortality in the 100 mg/L test medium when the pH was adjusted to 7.8 prior to the addition of fish.
The 96 -hour LC50 was 31.6 mg/L (geometric mean), the corresponding no-observed effect concentration (NOEC) was 21.5 mg/L.
If the results are based on the pH adjusted medium the 96 hour LC would be >100 mg/L and the corresponding NOEC would be 100 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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