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Diss Factsheets
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EC number: 258-380-0 | CAS number: 53126-67-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
Please refer to the attached read-across justification in section 13. - Reason / purpose for cross-reference:
- read-across source
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992-10-12 to 1992-10-16
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Molecular weight: 210.2 g/mol
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Application of test substance: direct weighing.
Undissolved particles of test substance sedimented on the ground of the vessel, but were dissolved after 15 minutes. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- - Origin: West-Aquarium (Bad Lauterberg, Germany)
- Body length: 2.5 - 3.5 cm
- Mortality during cultivation: < 2 %
- Culture water (medium): synthetic fresh water in arccordance with ISO - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 10.9 °dH
- Test temperature:
- 20.9 - 21.4 °C
- pH:
- 7.2 - 8.2
- Dissolved oxygen:
- 7.8 - 9.1 (90.5 - 104.2 %)
- Nominal and measured concentrations:
- Nominal conc: 100 mg/L (direct weighing), no measured concentrations.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: aquarium
- Material, size, fill volume: glass, 300*135*200 mm, 5 L test medium
- Aeration: yes, air circulation
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light : 8 h darkness - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- No mortality was observed. After 2 hours, the test item-treated animals appeared somewhat lethargic.
- Results with reference substance (positive control):
- no positive control was tested.
- Sublethal observations / clinical signs:
Validity of the test
No mortality in the control occured during the test. The dissolved oxygen concentration remained above 90 % throughout the exposure period.
Table 1: abiotic parameters, test item
0 h
24 h
48 h
72 h
96 h
Temperature
21.4
20.9
20.7
21.2
21.2
Oxygen [mg/L]
7.8
8.6
9.1
8.3
8.2
Oxygen saturation [%]
90.5
98.9
104.2
96
94.8
pH
8.2
7.2
7.3
7.2
7.6
Table 2: abiotic parameters, control
0 h
24 h
48 h
72 h
96 h
Temperature
21.3
20.8
20.7
21.2
21.2
Oxygen [mg/L]
7.8
8.8
9.2
8.5
8.4
Oxygen saturation [%]
90.3
101
105.4
98.3
97.1
pH
8.2
7.7
7.7
7.4
7.5
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 of the test item on zebrafish was determined to be >100 mg/L after 96 hours exposure.
- Executive summary:
The acute toxicity of the test item to fish was conducted in a static test according to OECD 201 and EU Method C.1 (Acute Toxicity for Fish). 10 zebrafish (Danio rerio) were exposed to a limit concentration of 100 mg/L. The test solution was prepared by direct weighing of the test item in 5 L test medium. A concurrent control ran parallel. After 96 hours of exposure a LC0 value of ≥ 100 mg/L was obtained. The LC50 was determined to be >100 mg/L. No significant effects of the test item on the fish were observed. All reported values are based on the nominal concentration.
Referenceopen allclose all
Description of key information
The LC50 on zebrafish was determined to be >100 mg/L after 96 hours exposure.
Key value for chemical safety assessment
Additional information
No study on fish with the target substance is available. A read-across to Dibutyl hydrogen phosphate (CAS 107-66-4) was performed to address the endpoint of short-term toxicity to fish.
The acute toxicity of the source item to fish was conducted in a static test according to OECD 201 and EU Method C.1 (Acute Toxicity for Fish). 10 zebrafish (Danio rerio) were exposed to a limit concentration of 100 mg/L. The test solution was prepared by direct weighing of the source item in 5 L test medium. A concurrent control ran parallel. After 96 hours of exposure a LC0 value of ≥ 100 mg/L was obtained. The LC50 was determined to be >100 mg/L. No significant effects of the test item on the fish were observed. All reported values are based on the nominal concentration.
In conclusion, the source substance showed no significant toxic effects on the tested animals. Therefore, it is expected that the target substance has no significant toxic effects on fish and the LC50 after 96 h exposure is >100 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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