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Diss Factsheets
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EC number: 270-390-7 | CAS number: 68427-32-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Good quality study under GLP, however no chemical analyses.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- no chemical analyses
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- no chemical analyses
- Details on test solutions:
- Direct dispersion in water. 1.0 g of test material was dispersed in 1 litre of diluent to give a stock solution of
1000 mg/l from which serial dilutions were made to give the test series.
The test material was suspected to absorb to glassware and so saturation of the absorption sites was achieved by soaking
the test vessels overnight prior to the start of the test with the test solutions. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Laboratory culture originating from a strain supplied by the Institut National de Recherche Chimique Appliquke, (I.R.CH.A), France. Cultures were fed
daily with a suspension of mixed algae (predominantly Chlorell a spp.) . Culture conditions ensure that reproduction is by parthenogenesis.
Gravid adults were isolated 24 hours prior to initiation of the test. Young daphnids produced overnight- were used for testing. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- no
- Hardness:
- Approximately 50 mg/L as CaCO3
- Test temperature:
- 22 ºC
- pH:
- 7.2-7.6
- Dissolved oxygen:
- 8.4 - 8.5 mgO2/L
- Nominal and measured concentrations:
- nominal concentrations:0, 3.2, 5.6, 10, 18, 32, 56, 100, 180 and 320 mg/l
- Details on test conditions:
Test vessels: glass jars each containing 200 ml test solution and covered to reduce evaporation
Experimental design: 9 test concentrations plus 1 control, each in duplicate. 20 animals per concentration.
Method of initiation: Daphnia's were placed in the test solution after addition of the test material.
Loading: 20 ml test solution per organism
Photoperiod: 16h light 8h dark
Temperature: 22ºC
Aeration: none
Test concentrations: 3.2, 5.6, 10, 18, 32, 56, 100, 180 and 320 mg/l
Mediurn renewal: none
Duration o f exposure :48 hours
Criteria of effect: Daphnia were considered to be immobilised if they were unable to swim for approximately 15 seconds after gentle agitation.- Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 38 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 % CL (32-44 mg/L)
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 15.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- no
- Validity criteria fulfilled:
- yes
- Conclusions:
- Good quality study, however no chemical analyses therefore concentrations nominal.
- Executive summary:
TEST SPECIES: Daphnia Magna Straus
TEST TYPE: Acute toxicity 48h EC50 (immobilisation)
TEST CONDITIONS: static test conditions according to OECD Guideline No. 202 Part 1 referenced as Method C. 2 of Commission Directive 84/449/EEC (which constitutes Annex V of Council Directive 67/584/EEC)
RESULTS:
EC50 (48h) 38 mg/L 15.2 mg/L ai
ai = active ingredient "No Observed Effect Concentration" (NOEC) 18mg/l (7.2 mg ai / l )
Reference
Concentration (mg/L) |
Cumulative immobilized Daphnia (initial population : 10 per concentration) |
|||||||
24 hours |
48 hours |
|||||||
R1 |
R2 |
Total |
% |
R1 |
R2 |
Total |
% |
|
3.2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
5.6 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
10 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
18 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
32 |
0 |
0 |
0 |
0 |
3 |
4 |
7 |
35 |
56 |
2 |
4 |
6 |
30 |
9 |
8 |
17 |
85 |
100 |
8 |
7 |
15 |
75 |
10 |
10 |
20 |
100 |
180 |
10 |
10 |
20 |
100 |
10 |
10 |
20 |
100 |
320 |
10 |
10 |
20 |
100 |
10 |
10 |
20 |
100 |
R1 and R2 = replicate 1 and 2
Description of key information
In a study according to OECD Guideline No. 202 Part 1 referenced as Method C. 2 of Commission Directive 84/449/EEC (which constitutes Annex V of Council Directive 67/584/EEC) the EC50 (48h) 38 mg/L (15.2) mg/L ai was determined.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.