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EC number: 614-598-1 | CAS number: 68553-12-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 9 April to 5 July 2017 (experimental dates)
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- 2006
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Batch No.of test material: RL686/15 (Elementis Specialties)
- Expiration date of the lot/batch: 30 October 2018
- Purity test date: 11/11/2015
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Control and 100% v/v saturated solution
- Sample storage conditions before analysis: Frozen - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test substance heated to 40oC to aid weighing. Test sustance was dissolved in test media and stirred at approximately 15000 rpm for 24 hours. After 24 hours stirring was stopped and undissolved test substance removed by filtration (0.2 um Sartorius Sartopore filter
Eluate: Reconstituted culture medium (OECD)
- Differential loading: No
- Controls: Media control only
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): None
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): 100% v/v saturated solution
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No data - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitiata
- Strain: CCAP 278/4
- Source (laboratory, culture collection): Master cultures maintained in laboratory. Orginal culture obtained from CCAP, SAMS Research Services Ltd, Scottish Marine Institute, Scotland, UK
ACCLIMATION
- Acclimation period: None - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Hardness:
- No data
- Test temperature:
- 24 +/-1oC (niominal)
- pH:
- 0h: 7.4-7.6
72h: 7.6 - Dissolved oxygen:
- No data
- Salinity:
- No data
- Conductivity:
- No data
- Nominal and measured concentrations:
- Nominal: Control and 100% v/v saturated solution
Measured:- Details on test conditions:
- TEST SYSTEM
- Test vessel: Conical glass flask
- Type (delete if not applicable): Closed with polyurethane foam stoppers
- Material, size, headspace, fill volume: Glass conical flask with approximately 100 mL test solution
- Aeration: No
- Type of flow-through (e.g. peristaltic or proportional diluter): Not applicable
- Initial cells density: 5 x 10^3 cells/mL
- Control end cells density: 6.61 x 10^5 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: Yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: RO deionised water
OTHER TEST CONDITIONS
- Sterile test conditions: No
- Adjustment of pH: No
- Photoperiod: Continuous illumination
- Light intensity and quality: approx 7000 lux (warm white lighting)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter]
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Limit test
- Justification for using less concentrations than requested by guideline: Limit test
- Range finding study
- Test concentrations: 0.1, 1.0, 10 and 100% v/v saturated solution
- Results used to determine the conditions for the definitive study: Yes- Reference substance (positive control):
- yes
- Remarks:
- Conducted 28 November to 01 December 2016; results within normal range
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 1.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 1.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
Nominal concn (% v/v saturated solution)
Geometric mean measured concn
(mg/L)
Growth rate
Yield (cells/mL)
0-72h
% inhibition
0-72h
% inhibition
Control
<LoQ
Mean
0.068
-
6.54 x 105
-
SD
0.0
-
1.21 x 104
-
100
1.1
Mean
0.065
5
5.22 x 105
20
SD
0.001
-
1.24 x 104
-
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 72-hour toxicity, based on growth rate, gave a 72-hour EC50 >1.1 mg/L (geometric mean measured concns) and a NOEC of 1.1 mg/L, although the test substance is not toxic to aquatic organisms at the limit of solubility, which is was less than the LoQ.
- Executive summary:
In a 72-h toxicity study the green alga,Pseudokirchneriella subcapitata, was exposed to a single test concentration under static conditions in accordance with the OECD test guideline 201 and in compliance with GLP. The nominal test concentrations were a control and a 100% v/v saturated solution prepared at a nominal loading rate of 50 mg/L. The measured concentrations at 0 and 72-hour were 8.9 and below the LoQ (Limit of Quantification), respectively.
Under the conditions of the test, the EC50 was determined to be >1.1 mg/L based on geometric mean measured concentrations. The NOEC was calculated to be 1.1 mg/L. However, the test substance is not toxic to aquatic organisms at the limit of solubility, which is was less than the LoQ.
Reference
Description of key information
In a 72-h acute toxicity study the green alga, Pseudokirchneriella subcapitata, was exposed to a single test concentration under static conditions in accordance with the OECD test guideline 201 and in compliance with GLP. The nominal test concentrations were a control and a 100% v/v saturated solution prepared at a nominal loading rate of 50 mg/L. Under the conditions of the test, the EC50 was determined to be >1.1 mg/L based on geometric mean measured concentrations. The NOEC was calculated to be 1.1 mg/L. However, the test substance is not toxic to aquatic organisms at the limit of solubility, which is was less than the LoQ.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1.1 mg/L
- EC10 or NOEC for freshwater algae:
- 1.1 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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