Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report Date:
1990

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Fingerling rainbow trouts were exposed for 48 hours to different concentrations of the test substance at a water temperature of 14 ± 1 °C. The no-observed-effect-levels and lethal concentrations (LC100) were determined.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: rainbow trouts
- Length at study initiation (length definition, mean, range and SD): 4 to 8 cm

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The substance was defined as barely toxic (i.e. no toxic effects observed up to 1000 mg/L) on the basis of a 48-hour test on the acute toxicity to fish conducted with rainbow trouts.
Executive summary:

A study (presumably under static conditions) on the acute toxicity of beta-Carotene to rainbow trouts (Salmo gairdneri L., now Oncorhynchus mykiss) was conducted over a period 48 hours. Fingerlings of 4 to 8 cm body length were exposed to different concentrations of the test substance. The test temperature was 14 ± 1 °C. The substance was defined as barely toxic on the basis of the test results, i.e. no toxic effects were observed up to a (presumably nominal) test concentration of 1000 mg/L.