Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 293-411-1 | CAS number: 91079-24-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- The analysis has been run on one of the "Rape oil, sulfated, sodium salt" representative sample.
Please, see section 13.2 for further details justifing the read-across approach from this supporting substance. - Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Nominal loadings: 0, 1.0, 3.16, 10.0, 31.6 and 100.0 mg/mL
- Sampling method: Samples taken from each test vessel at test start (0 hours) and test end (after 72 hours)
- Sample storage conditions before analysis: in original contyainer, sat room temperature, protected from light. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: In view of the difficulties associated with poorly water soluble test items, a modification of the standard method for the preparation of aqueous media was performed, exposing the organisms to a Water Accommodated Fraction (WAF) of the test item, as endorsed by several regulatory authorities in cases where the test item is a complex mixture and is poorly soluble in water and in the permitted solvents. Aqueous media were prepared by mixing the test item with water for a prolonged period sufficient to ensure equilibration between the test item and the water phase. At the completion of mixing and following a 1-hour settlement period, the test item phase was separated by siphon and the test organisms exposed to the aqueous phase, the WAF (which may contain dissolved and/or suspended and/or emulsified fractions of the test item mixture). Exposures were expressed in terms of the original concentration of the test item in water at the start of the mixing period (loading rate) irrespective of the actual concentration of the test item in the WAF. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Desmodesmus subspicata CHODAT SAG 86.81
- Source (laboratory, culture collection): Sammlung von Algenkulturen (SAG), Pflanzenphysiologisches Institut der Universität Göttingen, Nikolausberger Weg 18, D-37073 Göttingen
- Age of inoculum (at test initiation): 4-days
- Method of cultivation at test facility: Fresh stocks are prepared every month on Z-Agar. Light intensity amounts to 35-70 µE · m-2 · s-1 for 24 h per day.
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): yes, OECD medium
- Any deformed or abnormal cells observed: No - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No
- Hardness:
- Nominal hardness of 0.24 mmol Ca+Mg/L
- Test temperature:
- min. 21 deg C; max 23 deg C
- pH:
- The pH-value at the beginning of the test was measured in one additional replicate per loading level and the control. At the end of the exposure, it was measured in pooled replicates per loading and the control.
At test-start the values were between 7.89 and 7.91 for the test concentrations and 8.00 for the control.
At test-end ther values were between 8.10 to 8.29 for the test concentrations and 8.28 for the control. - Dissolved oxygen:
- No data
- Salinity:
- According to the guideline - OECD medium used
- Nominal and measured concentrations:
- Nominal concentrations : 0, 1.0, 3.16, 10.0, 31.6 and 100.0 mg/L
Measured concentrations : At start of the test (0 hr), ; at end of the test (72 hr), mg/L respectively - Details on test conditions:
- TEST SYSTEM
- Test vessel: Sterile 250 mL Erlenmeyer flask, sealed with cotton wool plugs, allowing ventilation.
- Material, size, headspace, fill volume: 100 mL OECD medium in a 250 mL Erlenmeyer flask
- Aeration: Rotary shaker at approximately 70 rpm
- Type of flow-through (e.g. peristaltic or proportional diluter): N/A; static
- Renewal rate of test solution (frequency/flow rate): N/A
- Initial cells density: Nominal: 2E3-5E3 cells/mL; Actual: 4332 cells/mL
- Control end cells density: Control mean cell density increased 196-fold after 72 h
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes - OECD medium
- Detailed composition if non-standard medium was used: N/A
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: OECD medium
- Intervals of water quality measurement: 0 & 72h
OTHER TEST CONDITIONS
- Sterile test conditions: yes - filtered before use
- Adjustment of pH: yes. All test concentrations were acidifed with 2 N HCl (150 µL/30mL)
- Photoperiod: 24 hours/day light
- Light intensity and quality: Nominal 60 - 120 µE · m-2 · s-1; Actual min. 60.2 µE · m-2 · s-1; max 72.2 µE · m-2 · s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Growth inhibition at 0, 24, 48 & 72h,
- Determination of cell concentrations: Fluorescence measurements at 0, 24, 48 and 72 hours. Chlorophyll-a-fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as background signal
TEST CONCENTRATIONS
- Spacing factor for test concentrations: geometric series with a factor of square-root of 10 (3.16)
- Justification for using less concentrations than requested by guideline: N/A
- Range finding study: Yes
- Test concentrations: 1.0, 10.0 and 100.0 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 17.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 13.7-22.6mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 3.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 70.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 66.1 - 75.1 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 3.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): none
- Any stimulation of growth found in any treatment: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: WAF approach used
- Effect concentrations exceeding solubility of substance in test medium: Yes - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: 0.59 mg/L (0.564-0.623 mg/L) - based on growth rate inhibitrion, 72h, nominal concentration
- EC50: 0.299 mg/L (0.278-0.324 mg/L) - based on yield inhibition, 72h, nominal concentration - Reported statistics and error estimates:
- The percentage inhibitions of the mean growth rate or yield based on the mean growth rate or yield of the control were calculated for each loading rate. The values which are close to x% were used for the determination of the ELx via linear interpolation using a plot with a logarithmic scale for the concentration versus the percentage of inhibition.
Dunnett’s test (one-sided) was carried out at a 95 % significance level, based on the assumption that each higher tested concentration must have at least the same or a stronger effect than the LOELR. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions in which this study was conducted, using the WAF approach endorsed for complex substances with a low solubility, the EL50 values for inhibition of growth rate (ErL50) and yield (EyL50) resulted to be 70.4 and 17.4mg/L, respectively. The NOELR for inhibition of growth rate (ErC0) and yield (EyC0) was 3.16 mg/L, the LOELR 10.0 mg/L.
- Executive summary:
Algal growth inhibition on an analogue of the substance (Rape oil, sulfated, sodium salt) has been investigated in a 72-hour test according to OECD/EU test method (OECD 201, 2011), with Desmodesmus subspicatus. The WAF apporach for complex substances with a low solubility has been followed. The EL50 values for inhibition of growth rate (ErC50) and yield (EyC50) were 70.4 and 17.4 mg/L, respectively. The NOELR for inhibition of growth rate (ErC0) and yield (EyC0) was 3.16 mg/L, the LOELR 10.0 mg/L.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Nominal loadings: 0, 1.0, 3.16, 10.0, 31.6 and 100.0 mg/mL
- Sampling method: Samples taken from each test vessel at test start (0 hours) and test end (after 72 hours)
- Sample storage conditions before analysis: in original contyainer, sat room temperature, protected from light. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: In view of the difficulties associated with poorly water soluble test items, a modification of the standard method for the preparation of aqueous media was performed, exposing the organisms to a Water Accommodated Fraction (WAF) of the test item, as endorsed by several regulatory authorities in cases where the test item is a complex mixture and is poorly soluble in water and in the permitted solvents. Aqueous media were prepared by mixing the test item with water for a prolonged period sufficient to ensure equilibration between the test item and the water phase. At the completion of mixing and following a 1-hour settlement period, the test item phase was separated by siphon and the test organisms exposed to the aqueous phase, the WAF (which may contain dissolved and/or suspended and/or emulsified fractions of the test item mixture). Exposures were expressed in terms of the original concentration of the test item in water at the start of the mixing period (loading rate) irrespective of the actual concentration of the test item in the WAF. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Desmodesmus subspicata CHODAT SAG 86.81
- Source (laboratory, culture collection): Sammlung von Algenkulturen (SAG), Pflanzenphysiologisches Institut der Universität Göttingen, Nikolausberger Weg 18, D-37073 Göttingen
- Age of inoculum (at test initiation): 4-days
- Method of cultivation at test facility: Fresh stocks are prepared every month on Z-Agar. Light intensity amounts to 35-70 µE · m-2 · s-1 for 24 h per day.
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): yes, OECD medium
- Any deformed or abnormal cells observed: No - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No
- Hardness:
- Nominal hardness of 0.24 mmol Ca+Mg/L
- Test temperature:
- min. 21 deg C; max 23 deg C
- pH:
- The pH-value at the beginning of the test was measured in one additional replicate per loading level and the control. At the end of the exposure, it was measured in pooled replicates per loading and the control.
At test-start the values were between 7.89 and 7.91 for the test concentrations and 8.00 for the control.
At test-end ther values were between 8.10 to 8.29 for the test concentrations and 8.28 for the control. - Dissolved oxygen:
- No data
- Salinity:
- According to the guideline - OECD medium used
- Nominal and measured concentrations:
- Nominal concentrations : 0, 1.0, 3.16, 10.0, 31.6 and 100.0 mg/L
Measured concentrations : At start of the test (0 hr), ; at end of the test (72 hr), mg/L respectively - Details on test conditions:
- TEST SYSTEM
- Test vessel: Sterile 250 mL Erlenmeyer flask, sealed with cotton wool plugs, allowing ventilation.
- Material, size, headspace, fill volume: 100 mL OECD medium in a 250 mL Erlenmeyer flask
- Aeration: Rotary shaker at approximately 70 rpm
- Type of flow-through (e.g. peristaltic or proportional diluter): N/A; static
- Renewal rate of test solution (frequency/flow rate): N/A
- Initial cells density: Nominal: 2E3-5E3 cells/mL; Actual: 4332 cells/mL
- Control end cells density: Control mean cell density increased 196-fold after 72 h
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes - OECD medium
- Detailed composition if non-standard medium was used: N/A
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: OECD medium
- Intervals of water quality measurement: 0 & 72h
OTHER TEST CONDITIONS
- Sterile test conditions: yes - filtered before use
- Adjustment of pH: yes. All test concentrations were acidifed with 2 N HCl (150 µL/30mL)
- Photoperiod: 24 hours/day light
- Light intensity and quality: Nominal 60 - 120 µE · m-2 · s-1; Actual min. 60.2 µE · m-2 · s-1; max 72.2 µE · m-2 · s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Growth inhibition at 0, 24, 48 & 72h,
- Determination of cell concentrations: Fluorescence measurements at 0, 24, 48 and 72 hours. Chlorophyll-a-fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as background signal
TEST CONCENTRATIONS
- Spacing factor for test concentrations: geometric series with a factor of square-root of 10 (3.16)
- Justification for using less concentrations than requested by guideline: N/A
- Range finding study: Yes
- Test concentrations: 1.0, 10.0 and 100.0 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 17.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 13.7-22.6mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 3.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 70.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 66.1 - 75.1 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 3.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): none
- Any stimulation of growth found in any treatment: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: WAF approach used
- Effect concentrations exceeding solubility of substance in test medium: Yes - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: 0.59 mg/L (0.564-0.623 mg/L) - based on growth rate inhibitrion, 72h, nominal concentration
- EC50: 0.299 mg/L (0.278-0.324 mg/L) - based on yield inhibition, 72h, nominal concentration - Reported statistics and error estimates:
- The percentage inhibitions of the mean growth rate or yield based on the mean growth rate or yield of the control were calculated for each loading rate. The values which are close to x% were used for the determination of the ELx via linear interpolation using a plot with a logarithmic scale for the concentration versus the percentage of inhibition.
Dunnett’s test (one-sided) was carried out at a 95 % significance level, based on the assumption that each higher tested concentration must have at least the same or a stronger effect than the LOELR. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions in which this study was conducted, using the WAF approach endorsed for complex substances with a low solubility, the EL50 values for inhibition of growth rate (ErL50) and yield (EyL50) resulted to be 70.4 and 17.4mg/L, respectively. The NOELR for inhibition of growth rate (ErC0) and yield (EyC0) was 3.16 mg/L, the LOELR 10.0 mg/L.
- Executive summary:
Algal growth inhibition has been investigated in a 72-hour test according to OECD/EU test method (OECD 201, 2011), with Desmodesmus subspicatus. The WAF apporach for complex substances with a low solubility has been followed. The EL50 values for inhibition of growth rate (ErC50) and yield (EyC50) were 70.4 and 17.4 mg/L, respectively. The NOELR for inhibition of growth rate (ErC0) and yield (EyC0) was 3.16 mg/L, the LOELR 10.0 mg/L.
Referenceopen allclose all
Description of key information
Toxicity to aquatic algae: EC50 -100 mg/L
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 70 mg/L
Additional information
Algal growth inhibition has been investigated in a 72-hour test according to OECD/EU test method (OECD 201, 2011), with Desmodesmus subspicatus, on Rape oil, sulfated, sodium salt - an analogue of the substance. The WAF approach for complex substances with a low solubility has been followed. The EL50 values for inhibition of growth rate (ErC50) and yield (EyC50) were 70.4 and 17.4 mg/L, respectively. The NOELR for inhibition of growth rate (ErC0) and yield (EyC0) was 3.16 mg/L, the LOELR 10.0 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)