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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2017.12.20-2018.01.17
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
GLP study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Lot no: 170604470
Purity: 92.68%
Molecular formula: C18H21N3O9
Expiration date: 2018.06.07
Transparant liquid
Oxygen conditions:
aerobic
Inoculum or test system:
other: Innoculum was collected from 12 sites in Korea including 5 rivers (Gangjeong, Mulgeum, Gurye, Gapcheon, Gokgyo), 4 domestic sewage treatment plants (Dalsco, Gumi, Busan, Asan) and 3 industrial waste water plants (Dalsco, Yeosu, Tancheon).
Details on inoculum:
Inoculum used in this study was collected from 12 sites in Korea including 5 rivers, 4 domestic sewage treatment plants and 3 industrial waste water plants. Fresh samples from these sites were incubated for over one month and the activated sludge greater than 10E7 cells/ml was used in this test
Duration of test (contact time):
28 d
Initial conc.:
15 mg/L
Based on:
other: BOD
Details on study design:
25±1 °C
Reference substance:
aniline
Key result
Parameter:
other: BOD measurements
Value:
16.6
Sampling time:
28 d
Key result
Parameter:
other: residual ammoun of test substance (HPLC analysis)
Value:
24.8
Sampling time:
28 d
Details on results:
The test substance is not readily biodegradable
Results with reference substance:
The percentage biodegradation of the reference substance (Aniline) was 76.0% after 7 days and 82.1% after 14 days and within the acceptable range (greater than 40% after 7 days and 65% after 14 days) specified in the OECD Guideline
Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
Based on the BOD measurement a biodegradation of 16.6 % was found after 28 days, which is below the pass level of 60% for ready biodegradability. The test substance is therefore considered as not readily biodegradable.
Executive summary:

The biodegradability of the test item Miramer S512 was studied according to the OECD Guideline no 301C following GLP.

Based on the BOD measurement a biodegradation of 16.6 % was found after 28 days, which is below the pass level of 60% for ready biodegradability. The test substance is therefore considered as not readily biodegradable.

Description of key information

The biodegradability of the test item Miramer S512 was studied according to the OECD Guideline no 301C following GLP.

Based on the BOD measurement a biodegradation of 16.6 % was found after 28 days, which is below the pass level of 60% for ready biodegradability. The test substance is therefore considered as not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed
Type of water:
freshwater

Additional information

The biodegradability of the test item Miramer S512 was studied according to the OECD Guideline no 301C following GLP.

Based on the BOD measurement a biodegradation of 16.6 % was found after 28 days, which is below the pass level of 60% for ready biodegradability. The test substance is therefore considered as not readily biodegradable.