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EC number: 905-357-4
CAS number: -
Mean OD570 Values and Percentage Viabilities for the Negative Control
Item, Positive Control Item and Test Item:
Absolute value of the difference of the rel. absorbances (%)
Positive Control Item
OD = Optical Density
The possible eye irritation potential of the substance was tested
in an in vitro test. Two type of tissues can be used either primary
human epidermal keratinocytes or human immortalized corneal epithelial
cells. The epidermal keratinocytes were used in the present test. The
study procedures described in this report were according to OECD TG 492
guideline and GLP principles. Skin tissue was treated by topical
application of 50 μL test substance for 30 minutes. After 120 hours
incubation period, determination of the cytotoxic (irritancy) effect was
performed. Cytotoxicity is expressed as the reduction of mitochondrial
dehydrogenase activity measured by formazan production from
(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) MTT at
the end of treatment. Eye irritation is expressed as the remaining cell
viability after exposure to the test substance. Reliable negative and
positive controls were included. The positive control had a mean cell
viability of 34.9%. The relative mean tissue viability obtained after 30
minutes treatment with the substance compared to the negative control
tissue was 98.6% presenting absence of eye irritation.
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