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EC number: 236-502-3 | CAS number: 13410-58-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 April 2004 to 06 May 2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1993
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Version / remarks:
- 1986
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Activated Sludge Respiration Inhibition Test, Annex to EEC Directive 88/302/EEC
- Version / remarks:
- 1987
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test material was added in the required amount to the test vessel with about 180 mL deionised water. The mixture was stirred for 22 ± 2 hours to obtain an optimal solution.
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Activated sludge was from the laboratory of a wastewater plant treating municipal sewage.
- The inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated during the night.
- 50 mL were added to a total volume of 250 mL to obtain a concentration of 1 g/L dry substance in the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 180 min
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.2 - 8.1 (after correction)
- Nominal and measured concentrations:
- Nominal: 100, 500 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250 mL)
- Fill volume: 250 mL
- Oxygen concentration during aeration: > 2.5 mg/L
- Oxygen concentration immediately before measurement: > 6.5 mg/L
- Duration of the measurement of oxygen consumption: 8 - 10 min
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3
- No. of vessels per reference material concentration (replicates): 1
- Sludge concentration: 1.0 g/L
- Synthetic medium: 8 mL/vessel, 100-fold concentrated OECD medium
OTHER TEST CONDITIONS
- Adjustment of pH: yes
EFFECT PARAMETERS MEASURED: Oxygen consumption
TEST CONCENTRATIONS
- 100, 500 and 1000 mg/L
VALIDITY CRITERIA
- Deviation of blank controls should be < 15%
- EC50 of the reference material (3,5-dichlorophenol) should be in the range of 5 - 30 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 180 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 180 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- ca. 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 180 min
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- VALIDITY CRITERIA
- Deviation of blank controls was < 15%
- EC50 of the reference material (3,5-dichlorophenol) was in the range of 5 - 30 mg/L
- The test is therefore valid - Results with reference substance (positive control):
- - After 180 minutes incubation the following results were recorded:
- EC20: < 1 mg/L
- EC50: About 5.2 mg/L
- EC 80: About 43 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this study the EC50 of the test material was > 1000 mg/L.
- Executive summary:
The toxicity of the test material to microorganisms was investigated in accordance with the standardised guidelines OECD 209 and ISO 8192-1986, under GLP conditions.
The determination of the Inhibition of Oxygen Consumption by Activated Sludge was assessed in the Activated Sludge Respiration Inhibition Test. Activated sludge from laboratory wastewater plant treating municipal sewage was used at a concentration of 1 g/L.
The inoculum and the test material (at 100, 500 and 1000 mg/L), or reference material (3,5-dichlorophenol at 1, 10 or 100 mg/L)) were incubated for 180 minutes at 20 ± 2°C and oxygen consumption was measured.
Under the conditions of this study the EC50 of the test material was > 1000 mg/L. All of the validity criteria of the study were met.
Reference
Table 1: Summary of Results
|
Blank control |
Test material |
Reference material |
|||||||
1 |
2 |
3 |
Mean |
1 |
2 |
3 |
1 |
2 |
3 |
|
Concentration (mg/L) |
- |
- |
- |
- |
1000 |
500 |
100 |
1 |
10 |
100 |
Added volume of stock solution (mL/vessel) |
- |
- |
- |
- |
- |
- |
- |
0.5 |
5.0 |
50.0 |
Direct addition (mg/vessel) |
- |
- |
- |
- |
250.0 |
125.0 |
25.0 |
- |
- |
- |
Synthetic medium (mL/vessel) |
8 |
8 |
8 |
- |
8 |
8 |
8 |
8 |
8 |
8 |
Inoculum (mL/vessel) |
50 |
50 |
50 |
- |
50 |
20 |
50 |
50 |
50 |
50 |
Filled up to the total volume with deionised water (mL/vessel) |
250 |
250 |
250 |
- |
250 |
250 |
250 |
250 |
250 |
250 |
Oxygen concentration start (mg O2/L) |
6.9 |
7.0 |
6.8 |
- |
7.4 |
7.5 |
7.3 |
7.0 |
8.5 |
8.8 |
Oxygen concentration after 6 min (mg O2/L) |
4.3 |
4.6 |
4.3 |
- |
5.4 |
5.4 |
4.9 |
5.1 |
7.5 |
8.6 |
Oxygen consumption rate (mg O2/L*6 min) |
2.6 |
2.4 |
2.5 |
2.5 |
2.0 |
2.1 |
2.4 |
1.9 |
1.0 |
0.2 |
Oxygen consumption rate (mg O2/L*h) |
26 |
24 |
25 |
25 |
20 |
21 |
24 |
19 |
10 |
2 |
Specific oxygen consumption rate (mg O2/g*h) |
26 |
24 |
25 |
25 |
20 |
21 |
24 |
19 |
10 |
2 |
Change in oxygen consumption rate compared with the blank control (%) |
- |
- |
- |
- |
20 |
16 |
4 |
24 |
60 |
92 |
Description of key information
Under the conditions of the study the EC50 of the test material was determined to be > 1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
The toxicity of the test material to microorganisms was investigated in accordance with the standardised guidelines OECD 209 and ISO 8192-1986, under GLP conditions. The study was awarded a reliability score of 1 in accordance with the criteria set forth by Klimisch et al. (1997).
The determination of the Inhibition of Oxygen Consumption by Activated Sludge was assessed in the Activated Sludge Respiration Inhibition Test. Activated sludge from laboratory wastewater plant treating municipal sewage was used at a concentration of 1 g/L.
The inoculum and the test material (at 100, 500 and 1000 mg/L), or reference material (3,5-dichlorophenol at 1, 10 or 100 mg/L)) were incubated for 180 minutes at 20 ± 2°C and oxygen consumption was measured.
Under the conditions of this study the EC50 of the test material was > 1000 mg/L. All of the validity criteria of the study were met.
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