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EC number: 945-734-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Skin sensitisation: not sensitising based on a read across from Zenolide which was tested in a Buehler test (OECD TG 406).
Key value for chemical safety assessment
Skin sensitisation
Link to relevant study records
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 2018
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Read-across information.
- Justification for type of information:
- This information is retrieved from Zenolide. The read across rationale is presented in the Skin sensitisation Endpoint summary and the accompanying files are also attached there.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Reading:
- 1st reading
- Hours after challenge:
- 32
- Group:
- test chemical
- Dose level:
- 100%
- No. with + reactions:
- 0
- Total no. in group:
- 20
- Clinical observations:
- no effects
- Reading:
- other:
- Group:
- positive control
- Remarks on result:
- other: check report
- Reading:
- other:
- Group:
- negative control
- Remarks on result:
- other: check report
- Interpretation of results:
- other: Not a skin sensitiser
- Remarks:
- according to EU CLP (EC No. 1272/2008 and its amendments).
- Conclusions:
- Oenanthic ether is not a skin sensitiser based on read across from Zenolide,which was tested in an OECD TG 406.
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Remarks:
- The Buehler test is performed before the REACH regulation of 2016 came into force.
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1975
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study has been performed according to OECD TG 406 but not under GLP and therefore a reliability 2 is assigned.
- Justification for type of information:
- The Buehler test is performed before the REACH regulation of 2016 came into force. The Buehler test is considered sufficiently reliable because 100% substance has been used for induction and challenge.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 406 (Skin Sensitisation)
- Deviations:
- yes
- Remarks:
- no control group used
- GLP compliance:
- no
- Type of study:
- Buehler test
- Justification for non-LLNA method:
- The study was performed before the LLNA method was an OECD TG method.
- Species:
- guinea pig
- Strain:
- Pirbright-Hartley
- Sex:
- not specified
- Details on test animals and environmental conditions:
- Weight of animals 300g; Temp. 21°C ± 2°C; Humidity 45 - 55%
- Route:
- epicutaneous, occlusive
- Vehicle:
- unchanged (no vehicle)
- Concentration / amount:
- undiluted
- Adequacy of induction:
- highest technically applicable concentration used
- Route:
- epicutaneous, occlusive
- Vehicle:
- unchanged (no vehicle)
- Concentration / amount:
- undiluted
- Adequacy of challenge:
- highest non-irritant concentration
- No. of animals per dose:
- 20; one dose was used
- Details on study design:
- 0.5ml of the pure substance is applied to a plaster of 19*25 mm. This was then fixed with an Elastoplaster on the left site of the animal. This area was shaved. The animal was then fixed in a holder for 6 hours. This treatment was performe twice a week for three weeks
- Challenge controls:
- All animals were retreated on both sides 14 days after the last treatment. Plaster was removed after 6 hours and after another 2 hours the assessment was made.
- Positive control substance(s):
- no
- Positive control results:
- There are no positive or negative controls presented in the study.
- Key result
- Reading:
- 1st reading
- Hours after challenge:
- 32
- Group:
- test chemical
- Dose level:
- 100%
- No. with + reactions:
- 0
- Total no. in group:
- 20
- Clinical observations:
- no effects
- Key result
- Reading:
- other: check report
- Group:
- positive control
- Remarks on result:
- not measured/tested
- Key result
- Reading:
- other: check report
- Group:
- negative control
- Remarks on result:
- other: check report
- Interpretation of results:
- other: Not a skin sensitiser
- Remarks:
- according to EU CLP (EC No. 1272/2008 and its amendments).
- Conclusions:
- Under the conditions of this study the test substance is not sensitizing.
- Executive summary:
In a skin sensitisation study, performed similar to OECD guideline 406, twenty guinea pigs were dosed with 0.5 mL of the substance in an Elastoplaster on the left site of the animal for six hours. The treatment was repeated twice a week for three weeks. No positive control group was included in the study. Fourteen days after topical induction, challenge dosing for detection of sensitisation was performed on both left and right sides of the animals. For challenge undiluted test substance was used. The redness of the skin was measured with a refractometer and was assessed according to the Draize score. There were no deaths or clinical findings. Draize scores from 0 to 1 were observed for all 20 animals. A hair removal reaction was noted. There were no sensitisation differences between left and right side of the animals detected. No contact hypersensitivity was observed in this study.
- Endpoint:
- skin sensitisation: in vitro
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- an in vitro skin sensitisation study does not need to be conducted because adequate data from an in vivo skin sensitisation study are available
Referenceopen allclose all
Animals showed a normal clinical appearance throughout the study. No contact hypersensitivity was observed. Skin reaction and oedema formation was observed. The redness of the skin was measured with a refractometer and was assesed with Draize score. Results: Draize score 0 - 1 for all 20 animals with remark: hair removal reaction. Results refractometer shows average values of 135 (left) and 137 (right).
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not sensitising)
- Additional information:
Skin sensitisation is assessed based on read-across from Zenolide to Oenanthic ether. The executive summary of Zenolide's Buehler test is presented first, followed by the read-across rationale.
Zenolide and its skin sensitisation potential
In a skin sensitisation study, a Buehler test, performed similar to OECD guideline 406, twenty guinea pigs were dosed with 0.5 mL of pure Zenolide in an Elastoplaster on the left site of the animal for six hours. The treatment was repeated twice a week for three weeks. No positive control group was included in the study. Fourteen days after topical induction, challenge dosing for detection of sensitisation was performed on both left and right sides of the animals. For challenge undiluted Zenolide was used. The redness of the skin was measured with a refractometer and was assessed according to the Draize score. There were no deaths or clinical findings. Draize scores from 0 to 1 were observed for all 20 animals. A hair removal reaction was noted. There were no sensitisation differences between left and right side of the animals detected. No contact hypersensitivity was observed in this study.
The skin sensitising properties of Oenanthic ether using read across from Zenolide (CAS 54982-83-1)
Introduction and hypothesis for the analogue approach
Oenanthic ether consists of 3 main constituents and a number of impurities. All are ethyl esters, despite the name ether, of long chain carboxylic acids. The major constituent has a C12 chain, the two minor ones have C14 and C16 saturated carbon chain. For this substance there are no experimental skin sensitisation data.
In accordance with Article 13 of REACH, lacking information can be generated by other means i.e. applying alternative methods such as QSARs, grouping and read-across. For assessing the skin sensitizing properties of Oenanthic ether, the analogue approach is selected because for the closely related analogue Zenolide sensitisation information is available which can be used to read across.
Hypothesis: Oenanthic Ether has the same skin sensitising properties compared to Zenolide. The structural differences are not expected to affect the skin sensitising properties.
Available information: The source chemical Zenolide has been tested in a skin sensitisation study, a Buehler test, performed similar to OECD guideline 406. Twenty guinea pigs were dosed with 0.5 mL of the substance in an Elastoplaster on the left site of the animal for six hours. The treatment was repeated twice a week for three weeks. A positive control group was not included in the study and is not needed. Fourteen days after topical induction, challenge dosing for detection of sensitisation was performed on both left and right sides of the animals. For challenge undiluted test substance was used. The redness of the skin was measured with a refractometer and was assessed according to the Draize score. There were no deaths or clinical findings. Draize scores from 0 to 1 were observed for all 20 animals. A hair removal reaction was noted. There were no sensitisation differences between left and right side of the animals detected. No contact hypersensitivity was observed in this study and therefore Zenolide is not a skin sensitiser.This Buehler test is considered sufficiently reliable because up to 100% substance has been used for induction and challenge.
Target chemical and source chemicals
Chemical structures of the target chemical and the source chemical(s) are shown in the data matrix, including physico-chemical properties and available toxicologicalinformation. Furthermore, a full list of constituents of Oenanthic ether, including information relevant for read-across, is given in the data matrix.
Purity / Impurities
The purity and unidentified impurities of the target chemical and source chemical are not expected to influence the potential for skin sensitisation.
Analogue approach justification
According to Annex XI 1.5 read across can be used to replace testing when the similarity can be based on a common backbone and a common functional group. When using read across the result derived should be applicable for C&L and/or risk assessment and it should be presented with adequate and reliable documentation. The reasoning below fulfils this documentation.
Analogue selection: For Oenanthic etherZenolide was considered an appropriate analogue because Zenolide has a similar fatty acid type of acetic ester as Oenanthic ether and for Zenolide skin sensitisation information was available.
Structural similarities and differences:All constituents of Oenanthic ether and Zenolide contain ethyl esters and a long alkane chain (C8-C18 and C12, respectively) and therefore have a similar backbone and functional group: i.e. esters of long chain carboxylic acids. The difference is that all constituents of Oenanthic ether have linear alkyl chains, whereas Zenolide has a cyclic aliphatic alkyl chain, connected by Ethylene glycol. In view of this double ester this site is likely more electrophilic.
Toxico-kinetics: The dermal availability of Oenanthic ether is very similar or may be slightly less compared to Zenolide because the former has a several constituents with higher molecular weights and log Kow outside the favourable dermal absorption range.
Skin sensitisation reactivity profile:The reactive site of Oenanthic Ether and Zenolide is the ester bond which has only a non-branched alkyl group in its vicinity and therefore is expected to react very similar. If anything Zenolide is slightly more reactive because it has a double ester in the vicinity of each other (OECD Toolbox, 4.2). Therefore the prediction is ‘conservative’.
Uncertainty of the prediction: There are no other remaining uncertainties not already addressed above. In addition, the OECD Toolbox profiler (4.2) does not indicate protein binding for Oenanthic ether.
Data matrix
The relevant information on physico-chemical properties and toxicological characteristics are presented in the data matrix below.
Conclusions for hazard and risk assessment
For Oenanthic ether no skin sensitisation information was available. Zenolide and its skin sensitisation information can be used for read across. When using read across, the result derived should be applicable for C&L and/or risk assessment and be presented with adequate and reliable documentation. This latter documentation is presented here in the current document. For Zenolide a well conducted Buehler test is available (OECDTG 406, Rel. 2, tested up to 100%) resulting in absence of skin sensitisation. Therefore also Oenanthic ether is not a skin sensitiser.
Final conclusion on hazard and risk assessment:Oenanthic ether is not a skin sensitiser.
Data matrix to support the read across to Oenanthic ether from Zenolide for skin sensitisation
Chemical names for
Oenanthic ether#
ethyl octanoate (C8*)
ethyl decanoate (C10*)
ethyl dodecanoate (C12*)
ethyl tetra-decanoate (C14*)
ethyl hexa-decanoate (C16*)
ethyl octa-decanoate (C18*)
ethyl (9Z)-octadec-9-enoate (C18*)
ethyl (9Z,12Z)‐ octa-deca‐,12‐di enoate (C18*)
Zenolide
(‘C12’)
Target
Source
CAS#
106-32-1
110-38-3
106-33-2
124-06-1
628-97-7
111-61-5
544-35-4
111-62-6
5982-83-1
Structure
% in product
<10
<10
35-55
15-30
5-15
<10
<10
<10
EC No.
945-734-0#
259-423-6
Registration 2018
Registered
Vp (Pa)
0.12 meas)#
31.4
(est.)
5.70
(est.)
1.17
(est.)
0.34
(est.)
0.036
(est.)
0.0084
(est.)
0.0081
(est.)
0.0067
(est.)
0.028
(exp.)
WS (mg/L)
1.6 meas)#
45.6
(est.)
4.8
(est.)
0.41
(est.)
0.037
(est.)
0.0037
(est.)
0.0004
(est.)
0.0006
(est.)
0.0009
(est.)
75
(exp.)
Log Kow
4.6 meas)#
3.8
(est.)
4.8
(est.)
5.8
(est.)
6.8
(est.)
7.7
(est.)
8.4
(est.)
8.5
(est.)
8.3
(est.)
3.65
(exp.)
Human health
Skin sensitisation
Negative (Read across)
Negative (Read across)
Negative (Read across)
Negative (Read across)
Negative (Read across)
Negative (Read across)
Negative (Read across)
Negative (Read across)
Negative (OECD TG 406, Buehler, using 100%)
*The C’s are related to the chain length not the overall number of Cs (as would be presented in the Empirical formula); (est.) = estimated using EpiSuite; (exp.) = experimental;#In this column the values are for Oenanthic ether as such.
Justification for classification or non-classification
Based on the results, the substance does not need to be classified for skin sensitisation according to EU CLP (EC No. 1272/2008 and its amendments).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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