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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
Deviations:
not specified
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
Dicyclohexylamine
EC Number:
202-980-7
EC Name:
Dicyclohexylamine
Cas Number:
101-83-7
IUPAC Name:
N-cyclohexylcyclohexanamine
Details on test material:
Purity not specified

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
not specified
Details on test solutions:
test concentration: 3 (100, 1000, 10000 mg/l)

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
TEST ORGANISM: - mixed population of aquatic micro-organisms of a waste water treatment plant treating predominantly domestic sewage (Wupperverband - Wupper area water authority); 

Study design

Test type:
static
Water media type:
not specified
Total exposure duration:
3 h

Test conditions

Test temperature:
- temperature: 20 +/- 2 °C
pH:
pH: 7.5 - 8.2
Nominal and measured concentrations:
- test concentration of the activated sludge: 6 g dry matter/l
Details on test conditions:
- test concentration of the activated sludge: 6 g dry matter/l. - test parameter: respiration rate - aeration: permanent
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorphenol (1, 20 mg/l)

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
other: EC05
Effect conc.:
210 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
712 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
other: EC95
Effect conc.:
2 414 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 for respiration rate was 712 mg/L for 3 hrs.