Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 215-851-5 | CAS number: 1429-50-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- from 16 May 1979 to 30 May 1979
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- The study was conducted according to an appropriate national standard method. It was compliant with GLP and analytical monitoring was carried out
- Qualifier:
- according to guideline
- Guideline:
- other: EPA-660/3-75-009 (US EPA)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all concentrations at days 0, 2, 5, 7, 9 and 12
- Sampling method: the sample collection method is not reported, however samples were collected each Monday before water renewals to determine the stability of the substance. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: the test concentrations were prepared by transferring direct weights of the test substance to the test chambers. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout
- Source: Spring Creek hatchery in Lewiston, Montana, USA
- Length at study initiation (length definition, mean, range and SD): mean standard length 46.2 mm
- Weight at study initiation (mean and range, SD): mean weight 1.77 g
- Method of breeding: not reported
- Feeding during test
- Food type: commercial fish food (Rangen's)
- Amount: 3% body weight
- Frequency: daily
ACCLIMATION
- Acclimation period: 14 day
- Acclimation conditions (same as test or not): culture techniques as described by Brauhn et al.
- Type and amount of food: same as during test
- Feeding frequency: same as during test
- Health during acclimation (any mortality observed): <5% mortalities - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 14 d
- Hardness:
- 255 ppm as CaCO3
- Test temperature:
- 12⁰C
- pH:
- control range: 7.2 to 8
low dose medium range: 7.2 to 8.1
high dose range: 6.7 to 7.8 - Dissolved oxygen:
- >60%
- Nominal and measured concentrations:
- Nominal concentrations: 0, 37, 65, 120, 210 and 370 mg active acid/L.
Measured concentrations: 0, 35, 62, 114 , 205 and 345 mg active acid/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel: aquaria
- Material, size, headspace, fill volume: 40 L glass aquaria filled with 30 L of test solution
- Aeration: yes
- Renewal rate of test solution (frequency): 75% media was replaced on Monday, Wednesday and Friday
- No. of organisms per vessel: 30
- No. of vessels per concentration (replicates): 0
- No. of vessels per control (replicates): 0
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: laboratory well water
- Alkalinity: 369 ppm as CaCO3
- Conductivity: 50 uhmos/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: water quality measurements were done on each renewal date in the control, low and high concentrations.
OTHER TEST CONDITIONS
- Adjustment of pH: none reported
- Photoperiod: 16 h daylight
- Light intensity: not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : the fish were observed for mortality and abnormal behaviour initially and once every 24 hours for the 14 day period.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 3
- Range finding study: not reported - Reference substance (positive control):
- yes
- Remarks:
- Antimycin A
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 250 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Remarks:
- active acid
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: (220-280)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 250 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Remarks:
- active acid
- Basis for effect:
- mortality (fish)
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 114 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Remarks:
- activ acid
- Basis for effect:
- mortality (fish)
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 35 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Remarks:
- active acid
- Basis for effect:
- behaviour
- Details on results:
- - Observations on body length and weight: weight measurements of surviving fish at the end of the study yielded the following weight percentages of the control group mean weight: 35 mg/l, 128%,; 62 mg/l, 114%; 114 mg/l, 112% and 205 mg/l 97%.
- Other biological observations: Although no mortalities were observed at 114 mg/l, all test fish at this concentration exhibited loss of equilibrium within 30 minutes after beginning of the test. All test fish at this concentration recovered from this apparent initial toxicant shock, the same behavioral response was noted immediately following each solution change at 114, 205 and 345 mg/l.
- Mortality of control: 0% - Results with reference substance (positive control):
- - Mortality: 100% mortality at 0.0014 mg/l
- LC50: 0.000084 mg/l - Reported statistics and error estimates:
- Litchfield and Wilcoxon
- Sublethal observations / clinical signs:
Table 1. Percentage mortalities
Mean measured concentration (mg active acid/L) Days of exposure 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Control 0 0 0 0 0 0 0 0 0 0 0 0 0 0 35 0 0 0 0 0 0 0 0 0 0 0 0 0 0 62 0 0 0 0 0 0 0 0 0 0 0 0 0 0 114 0 0 0 0 0 0 0 0 0 0 0 0 0 0 201 17 17 17 17 17 17 17 17 17 17 17 17 17 17 350 97 97 97 97 97 97 97 97 97 97 97 97 97 97 - Validity criteria fulfilled:
- yes
- Conclusions:
- A 14 d and 96 h LC50 value of 250 mg active acid/l and a NOEC value of 114 mg/l have been determined for the effects of the test substance on the mortality of the freshwater fish O. mykiss based on mean measured concentrations.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- The study was conducted according to an appropriate OECD test guideline. It was not compliant with GLP and no analytical monitoring was carried out.
- Qualifier:
- according to guideline
- Guideline:
- other: "Methods for acute toxicity tests with fish, macroinvertebrates and amphibians" EPA-660/3-75-009 (US EPA)
- Deviations:
- not applicable
- Remarks:
- guideline not available
- GLP compliance:
- no
- Specific details on test material used for the study:
- Test material neutralised to pH 7.0 with NaOH.
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: an appropriate amount of test substance was added directly to the water in each vessel to obtain the desired concentration
- Controls: sea water only - Test organisms (species):
- Cyprinodon variegatus
- Details on test organisms:
- TEST ORGANISM
- Common name: sheepshead minnow
- Source: collected from Big Lagoon, near Bionomics Marine Research Laboratory (BMRL)
- Length at study initiation (length definition, mean, range and SD): 15-20 mm
- Feeding during test: no
ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: not reported
- Feeding frequency: not reported
- Health during acclimation (any mortality observed): <1% mortality - Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 20±1⁰C
- pH:
- initial pH range in exposure media: 5.7 to 6.9
Although the pH in highest test concentration was below the validity criterion, 5.8 to 5.9, it is not thought by the reviewer that this would have significantly affected the outcome of the study.
pH in controls: 8.2 to 8.3 - Dissolved oxygen:
- ≥60%
- Salinity:
- 25‰
- Nominal and measured concentrations:
- Nominal concentrations: control, 1800, 3200, 5600, 10 000 and 18 000 µ/l (ppm)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: jars
- Type (delete if not applicable): open
- Material, size, fill volume: 19 L glass jars, filled with 15L
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural, filtered sea water
- Culture medium different from test medium: no
- Intervals of water quality measurement: daily
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: not reported
- Light intensity: not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortalities were recorded every 24 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 1.8
- Range finding study: none reported - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 8 000 other: (µl/l) ppm
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: equivalent to 1600 mg active acid/L
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 9 960 other: µl/l (ppm)
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: equivalent to 2000 mg active acid/L
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 9 960 other: µl/l (ppm)
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: equivalent to 2000 mg active acid/L
- Reported statistics and error estimates:
- LC50s were calculated by linear regression
- Sublethal observations / clinical signs:
Table 1. Summary of mortalities.
Nominal concentrations ppm (µl/l) Mortality (%) 24 h 48 h 96 h Control 0 0 0 1800 0 0 10 3200 0 10 10 5600 0 0 10 10 000 30 30 40 18 000 100 100 100 The concentrations have been reported in terms of µl/l (ppm) and whole test substance. The test substance has a density of 1.21 and an active acid content of 16.3%. Therefore the results in terms of active acid are:
24 h LC50 9960 ppm (*1.21*16.3%) = 2000 mg active acid/L
48 h LC50 9960 ppm (*1.21*16.3%) = 2000 mg active acid/L
96 h LC50 8000 ppm (*1.21*16.3%) = 1600 mg active acid/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 96 h LC50 value of 8000 µl/l (ppm), equivalent to 1600 mg active acid/L has been reported for the effects of the test substance on the mortality of the marine fish C. variegatus.
Referenceopen allclose all
Description of key information
14 d and 96 h LC50: 250 mg active acid/l and NOEC: 114 mg active acid/l, O. mykiss, reliability 1 (ABC 1979).
96 h LC50: 1600 mg active acid/l, C. variegatus, reliability 2 (EG&G 1976).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 250 mg/L
Marine water fish
Marine water fish
- Effect concentration:
- 1 600 mg/L
Additional information
A 14 d and 96 h LC50 value of 250 mg active acid/l and NOEC value of 114 mg/l have been determined for the effects of the test substance on the mortality of the freshwater fish O. mykiss based on mean measured concentrations (ABC, 1979). This study has been selected as key as it is the lowest reliable LC50 value with freshwater fish.
A 96 h LC50 value of 8000 µl/l (ppm), equivalent to 1600 mg active acid/l has been reported for the effects of the test substance on the mortality of the marine fish C. variegatus (EG&G, 1976 (6.1.1.004)). This study has been selected as key as it is the lowest reliable LC50 value for marine fish.
A supporting study has also reported a 96 h LC50 value of 970 mg active acid/l determined for the effects of the test substance on the mortality of the marine fish Ictalurus punctatus, based on nominal concentrations (EG&G, 1976 (6.1.1.003)).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.