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EC number: 815-096-7 | CAS number: 1421927-53-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014-10-08 - 2014-10-10
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Principles of method if other than guideline:
- The study was performed by the Swiss Federal Laboratories for Materials Science and Technology (Empa), an it is an institution of the Swiss federation. The study was performed according to OECD 2012 but without GLP which does not affect the reliability of this study.
- GLP compliance:
- no
- Remarks:
- The study was performed by the Swiss Federal Laboratories for Materials Science and Technology (Empa), an it is an institution of the Swiss federation.
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- Since EMPA FR1 (EDA-DOPO) is poorly soluble, the water accommodated fraction (WAF) approach was used. The loadings were prepared by adding the respective amounts of test item to empty glass vessels, adding the aerated Daphnia medium, moderately stirring it for 24 h, and finally filtering it (MILLIPORE AP15 glass fiber filter). The resulting water soluble fractions (WSF) were used in the test.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia (Straus, 1820), aged less than 24 h, were acclimatized to the test medium for about 1 h before introduction into the formulated test media.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.52 mmol/l
- Test temperature:
- 20±2°C
- pH:
- 6.9 - 7.0
- Dissolved oxygen:
- 6.9 - 7.4 mg/l
- Conductivity:
- 711-794 µS *cm-1
- Nominal and measured concentrations:
- nominal: Water accomodated fraction loading rates: 10, 100, 1000 mg/l
- Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 24 and 48 hour LC50 of EDA-DOPO was determined in an acute Daphnia immobilization test according to OECD Guideline 202. 24 hour old Daphnids were exposed to the test item as a water accomodated fraction. The 24 and 48-hour LC 50 was determined as > 1,000 mg/l.
- Executive summary:
The median effective concentration (EC50) and the no-observed effect concentration (NOEC) of EMPA FR1 (EDA-DOPO) to Daphnia magnawere investigated under static exposure conditions over a period of 48 h. 40 individual Daphnia divided into 2 test vessels were exposed to the test item. Since EMPA FR1 (EDA-DOPO) is poorly soluble, the water accommodated fraction (WAF) approach was applied. No analytical verification of the test item was conducted. The evaluation of the test was based on the loading rates of the test item, assuming the test item to be stable in water over 48 h.The applied loading rates were 10, 100 and 1000 mg/l. After 24 and 48 h of incubation no significant effects (<10% of a total of 40 individuals) were observed at any of the 10, 100 and 1000 mg/l loadings rates and in the blank control.The medium effect concentration (EC50) for EMPA FR1 (EDA-DOPO) after 24 and 48 h of exposure was estimated to be >1000 mg/l loading rate.The no-observed effect concentration (NOEC) after 24 h and 48 h of exposure was shown to be 1000 mg/l loading rate. Commission Regulation (EU) No 286/2011 (i.e. CLP regulation) requires the use of the EC5048 h value for classification purposes. Based on an EC5048 h >100 mg/l,EMPA FR1 (EDA-DOPO) is neither regarded as toxic nor harmful to the daphnid Daphnia magna.
Reference
|
|
Number of |
Immobile |
Immobile |
% immobile |
% immobile |
Loading rate (mg/l) |
Replicate |
individuals per test |
Daphnia(No./vessel) |
Daphnia(No./vessel) |
Daphnia after 24 h of |
Daphnia after 48 h of |
|
|
vessel |
24h |
48h |
exposure |
exposure |
|
A |
20 |
0 |
0 |
0 |
0 |
Control0 |
B |
20 |
0 |
0 |
0 |
0 |
|
C |
20 |
0 |
0 |
0 |
0 |
10 |
A |
20 |
0 |
0 |
0 |
0 |
B |
20 |
0 |
0 |
0 |
0 |
|
100 |
A |
20 |
0 |
0 |
0 |
0 |
B |
20 |
0 |
0 |
0 |
0 |
|
1000 |
A |
20 |
0 |
1 |
0 |
5 |
B |
20 |
2 |
2 |
10 |
10 |
Description of key information
The 24 and 48 hour LC50 of EDA-DOPO was determined in an acute Daphnia immobilization test according to OECD Guideline 202. 24 hour old Daphnids were exposed to the test item as a water accomodated fraction. The 24 and 48-hour LC 50 was determined as > 1,000 mg/l.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.