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EC number: 947-399-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- of read across substance
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on two short term toxicity study of fish for the test chemical :
1. and 2.The study was designed to assess the toxic effects of the test compound on the ZEBRA FISH(Danio rerio) . - GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material: Reaction mass of Ethanaminium, N-[4-[[4-(diethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl- & acetate
- Molecular formula: C29H36N2O2
- Molecular weight: 444.615 g/mole
- Substance type: Organic
- Physical state: maroon lustrous liquid - Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Details on test solutions:
- 1)The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of potable water (passed through reverse osmosis system) with 1 hr.continuous stirring for achieving test concentrations of 1 g/L. Hence, concentrations are 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/L,respectively.
2) Preparation of the test Solutions : The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 1 hour continuous stirring. After that analytically detect stock,solubility found 981.56 mg/L and calculations done accordingly. Concentrations decided as follows 0.625mg/L,1.25mg/L,2.5mg/L,5mg/L,10mg/L,respectively. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- 1.TEST ORGANISM
- Common name:ZEBRA FISH
- Source:UNIQUE AQUARIUM, NAGPUR
- Length at study initiation (length definition, mean, range and SD):average 1.94 cm
- Weight at study initiation (mean and range, SD):0.1494 g (average)
ACCLIMATION
- Acclimation period:7 days
- Type and amount of food during acclimation:Standard brand feed
- Feeding frequency during acclimation:Daily once
- Health during acclimation (any mortality observed):During last 48 hours = 0
FEEDING DURING TEST
- Food type:Standard brand feed
- Frequency:Withdrawal of food 1 day before and during exposure
2.TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source:Koyal aquarium, Nagpur
- Age at study initiation (mean and range, SD): -
- Length at study initiation (length definition, mean, range and SD):average 1.79 cm
- Weight at study initiation (mean and range, SD):0.1204g (average)
- Method of breeding: -
- Feeding during test: -
- Food type:Standard brand feed,
- Frequency: once daily - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Hardness:
- 1) and 2) 148.5 mg of CaCO3
- Test temperature:
- 23 deg C
- pH:
- 7.7
- Salinity:
- 2) 6.41
- Nominal and measured concentrations:
- 1) 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/L
2) 0.625mg/L,1.25mg/L,2.5mg/L,5mg/L,10mg/L - Details on test conditions:
- 1) and 2) TEST SYSTEM
- Test vessel:The test vessels used were all plastic aquaria having the water capacity of 5liters.
- Material, size, headspace, fill volume: 2 liters
- No. of organisms per vessel:8 fishes
OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark
2) - Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.312 - < 0.625 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.625 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The test chemical Reaction mass of Ethanaminium, N-[4-[[4-(diethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl- & acetate is likely to be toxic to fish atleast in the concentration range of 0.312 to 0.625 mg/l
- Executive summary:
Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical Reaction mass of Ethanaminium, N-[4-[[4-(diethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl- & acetate .The studies are as mentioned below:
1) Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material. The nominal concentration selected for the experiment were 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/Land test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >0.3125 mg/L but <0.625 mg/L LC0 (96 hours) (highest loading at which no mortality was observed) = 0.3125 mg/L LC50 (96 hours) Experimental = >0.3125 mg/L but <0.625 mg/L .LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 0.625 mg/L
2) Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material .The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 1 hour continuous stirring. After that analytically detect stock,solubility found 981.56 mg/L and calculations done accordingly. Concentrations decided as follows 0.625mg/L,1.25mg/L,2.5mg/L,5mg/L,10mg/L,respectively.
Test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be 0.625 mg/L. LC50 (96 hours) Experimental = 0.625 mg/L .LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 1.25 mg/L.
Thus, based on the above summarised studies, Reaction mass of Ethanaminium, N-[4-[[4-(diethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl- & acetate and it’s structurally similar read across substance, it can be concluded that effect concetration value is in the range 0.312 to 0.625 mg/l
L. Thus, comparing this value with the criteria of CLP regulation, Reaction mass of Ethanaminium, N-[4-[[4-(diethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl- & acetate can be classified as "Acute aquatic-1" for short term toxicity for fish
Reference
Description of key information
Short - term toxicity to fish:
Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical Reaction mass of Ethanaminium, N-[4-[[4-(diethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl- & acetate .The studies are as mentioned below:
1) Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material. The nominal concentration selected for the experiment were 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/Land test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >0.3125 mg/L but <0.625 mg/L LC0 (96 hours) (highest loading at which no mortality was observed) = 0.3125 mg/L LC50 (96 hours) Experimental = >0.3125 mg/L but <0.625 mg/L .LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 0.625 mg/L
2) Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material .The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 1 hour continuous stirring. After that analytically detect stock,solubility found 981.56 mg/L and calculations done accordingly. Concentrations decided as follows 0.625mg/L,1.25mg/L,2.5mg/L,5mg/L,10mg/L,respectively.
Test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be 0.625 mg/L. LC50 (96 hours) Experimental = 0.625 mg/L .LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 1.25 mg/L.
Thus, based on the above summarised studies, Reaction mass of Ethanaminium, N-[4-[[4-(diethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl- & acetate and it’s structurally similar read across substance, it can be concluded that effect concetration value is in the range 0.312 to 0.625 mg/l
L. Thus, comparing this value with the criteria of CLP regulation, Reaction mass of Ethanaminium, N-[4-[[4-(diethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl- & acetate can be classified as "Acute aquatic-1" for short term toxicity for fish
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.625 mg/L
Additional information
Short - term toxicity to fish:
Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical Reaction mass of Ethanaminium, N-[4-[[4-(diethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl- & acetate .The studies are as mentioned below:
1) Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material. The nominal concentration selected for the experiment were 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/Land test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >0.3125 mg/L but <0.625 mg/L LC0 (96 hours) (highest loading at which no mortality was observed) = 0.3125 mg/L LC50 (96 hours) Experimental = >0.3125 mg/L but <0.625 mg/L .LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 0.625 mg/L
2) Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material .The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 1 hour continuous stirring. After that analytically detect stock,solubility found 981.56 mg/L and calculations done accordingly. Concentrations decided as follows 0.625mg/L,1.25mg/L,2.5mg/L,5mg/L,10mg/L,respectively.
Test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be 0.625 mg/L. LC50 (96 hours) Experimental = 0.625 mg/L .LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 1.25 mg/L.
Thus, based on the above summarised studies, Reaction mass of Ethanaminium, N-[4-[[4-(diethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl- & acetate and it’s structurally similar read across substance, it can be concluded that effect concetration value is in the range 0.312 to 0.625 mg/l
L. Thus, comparing this value with the criteria of CLP regulation, Reaction mass of Ethanaminium, N-[4-[[4-(diethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl- & acetate can be classified as "Acute aquatic-1" for short term toxicity for fish
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