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EC number: 200-644-4 | CAS number: 67-16-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 27 September 2004
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2014
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- not specified
Test material
- Reference substance name:
- N,N'-[dithiobis[2-(2-hydroxyethyl)-1-methylvinylene]]bis[N-[(4-amino-2-methyl-5-pyrimidinyl)methyl]formamide
- EC Number:
- 200-644-4
- EC Name:
- N,N'-[dithiobis[2-(2-hydroxyethyl)-1-methylvinylene]]bis[N-[(4-amino-2-methyl-5-pyrimidinyl)methyl]formamide
- Cas Number:
- 67-16-3
- Molecular formula:
- C24H34N8O4S2
- IUPAC Name:
- N,N'-[dithiobis[2-(2-hydroxyethyl)-1-methylvinylene]]bis[N-[(4-amino-2-methyl-5-pyrimidinyl)methyl]formamide
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- Purity of test material is not specified in the study report.
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- To prepare the stock solution, the test item was dissolved or dispersed directly in LC reconstituted water. The obtained preparation was agitated mechanically for up to 24 hours in an attempt to completely dissolve or disperse the test item and homogenize the solution. Water reconstituted (LC) was prepared using deionized water with a conductivity less than 10 PS/cm. The final solution after autoclaving has a pH of 7.5 ± 0.3.
Test organisms
- Test organisms (species):
- Scenedesmus sp.
- Details on test organisms:
- Strain No.: CCAP 276/20
Rapidly growing unicellular species commonly used in Europe for aquatic toxicity testing and recommended in OECD and EEC guidelines.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- Not specified
- Test temperature:
- between 23,2°C and 25,4°C
- pH:
- between 7,65 and 9,40
- Dissolved oxygen:
- Not specified
- Salinity:
- Not specified
- Conductivity:
- less than 10 PS/cm
- Nominal and measured concentrations:
- Nominal concentration : 0, 1, 10 & 100mg/L
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- The highest No Observed Effect Concentration on the growth rate (NOEC) at T72h was 100 mg/L, based on growth rate and biomass (p = 0.05).
The 0-72h EC50r and the 0-72h EC50b was > 100 mg/L.
Any other information on results incl. tables
Table 1: pH and temperature
Nominal charge rate (mg/L) |
pH at hour TO |
pH at hour T72 |
0 100 |
7,67 7,65 |
9,40 8,73 |
Temperature measured in water between T2 hours and TO time: 23.9°C
Temperature measured in a beaker of water dilution placed in the conditions of the study: minimum 23.20°C / maximum 25.40°C
Table 2: T72 hours - number of cells and calculation of the specific growth rate for each concentration
Nominal charge rate (mg/L) |
Replicat |
Total number of cells at T72 hours (104/mL) |
Ln number of cells |
Average Ln number of cellss |
Specific growth rate |
Inhibition of growth rate |
0 |
1 2 3 |
139,0 121,0 130,0 |
14,14 14,08 |
14,08 |
0,07 |
|
1 |
1 2 3 |
188,0 156,0 177,0 |
14,45 14,26 14,39 |
14,36 |
0,07 |
-5,92 |
10 |
1 2 3 |
112,0 127,0 133,0 |
13,93 14,05 14,10 |
14,03 |
0,07 |
0,99 |
100 |
1 2 3 |
104,0 122,0 136,0 |
13,85 14,01 14,12 |
14,00 |
0,07 |
1,62 |
Ln number of cells to T 0 hours (10,000 cells) = 9,21
Table 3: T72 hours - number of cells (104 cells x mL- l) at each observation time and calculation of the area under the growth curve (biomass) at each concentration.
Area = cells x mL-1 x hour
Nominal charge rate (mg/L) |
Replicat |
Total number of cells at T72 hours (104/mL) |
Growth O à 72 hours |
Average (0-72 hours) |
Growth inhibition |
|
1 2 3 |
139,0 121,0 130,0 |
4968,0 4320,0 4644,0 |
4644,0 |
|
1 |
1 2 3 |
188,0 156,0 177,0 |
6732,0 5580,0 6336,0 |
6216,o |
-33,85 |
10 |
1 2 3 |
112,0 127,0 133,0 |
3996,0 4536,0 4752,0 |
4428,0 |
4,65 |
100 |
1 2 3 |
104,0 122,0 136,0 |
3708,0 4356,0 4860,0 |
4308,0 |
7,24 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The highest No Observed Effect Concentration on the growth rate (NOEC) at T72h was 100 mg/L, based on growth rate and biomass (p = 0.05).
The 0-72h EC50r and the 0-72h EC50b was > 100 mg/L.
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