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EC number: 242-358-2 | CAS number: 18479-49-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02.10.2014 - 20.03.2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- GLP study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3,7-dimethyloct-1-en-3-ol
- EC Number:
- 242-358-2
- EC Name:
- 3,7-dimethyloct-1-en-3-ol
- Cas Number:
- 18479-49-7
- Molecular formula:
- C10H20O
- IUPAC Name:
- 3,7-dimethyloct-1-en-3-ol
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- For the determination of the actual test item concentrations, duplicate samples were taken from each treatment at the start and at the end of the test (after 48 hours).
For the 48-hour stability samples, the contents of the respective replicates were combined prior to sampling.
All samples were stored deep-frozen (at about -20 °C) immediately after sampling. Based on pre-experiments for investigation of the storage stability, the test item was found to be stable in the test water under these storage conditions.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Test Water
Reconstituted test water according to ISO 6341 [ISO, 1996] was used in the study. It consisted of analytical grade salts dissolved in purified water at the following nominal concentrations:
Ingredients / Concentration
Properties [mmol/L] [mg/L]
CaCl2 × 2H2O 2.0 294
MgSO4 × 7H2O 0.5 123
NaHCO3 0.75 65
KCl 0.075 5.8
Water Hardness 2.5 250 as CaCO3
Alkalinity 0.8 ---
The ratio of Ca:Mg and Na:K was 4:1 and 10:1, respectively, based on molarities.
The test water was aerated prior to the start of the study until oxygen saturation was reached. During the test period, the test water was not aerated.
The following nominal concentrations of 3,7-dimethyloct-1-en-3-ol were tested: 1.0, 3.2, 10, 32 and 100 mg/L. Additionally, a control (test water without test item) was tested in parallel.
Dosage
The test medium of the highest nominal concentration of 100 mg/L was prepared in a completely filled and tightly closed stirring vessel by completely dissolving 109.3 mg of test item in 1090 mL of test water using intense stirring for 3 hours at room temperature in the dark. Adequate volumes of this test medium were serially diluted with test water to prepare the lower concentrated test media. The test media were prepared just before the introduction of the daphnids (i.e., start of the test).
The preparation of the test media was based on the OECD Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures (2000).
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The study was performed with young daphnids of the species Daphnia magna Straus. A clone of this species (defined by the supplier as clone 5) was originally supplied by the University of Sheffield / UK in 1992. Since that time, the clone has been bred at Harlan Laboratories in reconstituted water of the quality identical to the water quality used in the tests (with respect to pH, main ions, and total hardness) and under temperature and light conditions identical to those of the test.
During breeding, daphnids are generally fed three times a week with an algal suspension of the green algae Desmodesmus subspicatus Chodat, Strain No. 86.81 SAG, supplied by the Collection of Algal Cultures (SAG, Institute for Plant Physiology, University of Göttingen, 37073 Göttingen / Germany) and cultivated at Harlan Laboratories under standardized conditions or a mixture of this algal suspension and a commercial fish diet (Tetra Min® Hauptfutter, supplied by TETRA-Werke, 49304 Melle / Germany).
At the start of the test, the organisms used in the test were 6-24 hours old and were not first brood progeny.
Study design
- Test type:
- static
- Water media type:
- other: Reconstituted test water according to ISO 6341
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- 21-22 °C
- pH:
- 7.8
- Dissolved oxygen:
- 8.4-9.0 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 0, 1.0, 3.2, 10, 32 and 100 mg/L
Measured concentrations (day 0): 0, 0.965, 3.08, 9.39, 33.0 and 95.8 mg/L
Measured concentrations (day 2): 0, 0.931, 2.99, 9.63, 32.5 and 93.8 mg/L - Details on test conditions:
- Experimental Conditions
The test was performed in a temperature-controlled room. The water temperature was maintained at 21-22 °C.
A 16-hour light to 8-hour dark cycle with a 30-minute transition period was used. Light intensity during the light period was approximately between 800 and 1060 Lux.
The daphnids were not fed during the test.
Study Design
In the definitive test, the enlarged spacing factor of 3.2 between the test concentrations was chosen based on the results of the range-finding test that documented a rather flat concentration-effect relationship; therefore, a large concentration range had to be tested. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 39 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The measured concentrations of 3,7-dimethyloct-1-en-3-ol in the test media of all test concentrations were between 94 and 103% of the nominal values at the start of the test and between 93 and 102% at the end of the test. Thus, the correct dosage of the test item 3,7-dimethyloct-1-en-3-ol was confirmed. The test item was stable in the test media over the test period of 48 hours. The biological results were related to the nominal concentrations of the test item.
- Results with reference substance (positive control):
- For evaluation of the quality of the daphnia clone and the experimental conditions, potassium dichromate is tested as a positive control twice a year. The result of the latest positive control test in August 2014 (48-hour EC50: 1.0 mg/L) showed that the sensitivity of the test organisms was within the internal historical range (48-hour EC50 from
2000 to 2014: 0.36-1.1 mg/L). - Reported statistics and error estimates:
- Using bionomial distribution, the 95% confidence limits for the 48h-EC50 were calculated as 10 and 100 mg/L.
Any other information on results incl. tables
The biological test results were as follows based on nominal concentrations:
Parameter (0-48 h) |
Immobility |
Nominal test item concentration [mg/L] |
|
24-hour EC50 |
51 |
95% confidence interval |
32 and 100 |
24-hour EC0 |
3.2 |
24-hour EC100 |
100 |
48-hour EC50 |
39 |
95% confidence interval |
10 and 100 |
48-hour EC0and |
|
48-hour NOEC |
1.0 |
48-hour EC100 |
100 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item 3,7-dimethyloct-1-en-3-ol had acute toxic effects on Daphnia magna. The 48-hour EC50 was determined to be 39 mg/L with 95%-confidence limits of 10 and 100 mg/L.
- Executive summary:
The acute toxicity of the test item 3,7-dimethyloct-1-en-3-ol to Daphnia magna was determined in a 48-hour static test according to the Commission Regulation (EC) No. 440/2008, Part C.2 and the OECD Guideline for Testing of Chemicals, No. 202 (2004).
The nominal test item concentrations tested were 1.0, 3.2, 10, 32 and 100 mg/L. Additionally, a control group was tested in parallel.
As the test item is a volatile substance, the test was performed using glass tubes completely filled with test medium, minimizing the headspace, and tightly sealed with glass stoppers to avoid losses of test item. The preparation of the test media was based on the OECD Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures, 2000.
The measured concentrations of 3,7-dimethyloct-1-en-3-ol in the test media of all test concentrations were between 94 and 103% of the nominal values at the start of the test and between 93 and 102% at the end of the test. The biological results were related to the nominal concentrations of the test item 3,7-dimethyloct-1-en-3-ol, since the correct dosage was confirmed.
The biological test results were as follows:
Parameter (0-48 h)
Immobility
Nominal test item concentration
[mg/L]
24-hour EC50
51
95% confidence interval
32 and 100
24-hour EC0
3.2
24-hour EC100
100
48-hour EC50
39
95% confidence interval
10 and 100
48-hour EC0and
48-hour NOEC
1.0
48-hour EC100
100
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