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EC number: 206-017-1 | CAS number: 288-13-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Oral: rat LD50: 1010 mg/kg bw. Reliability = 2
Dermal: rabbit LD50: 400 mg/kg. Standardized test protocol. Reliability = 2
Inhalation: rat 4 -hour LC50: >0.37 mg/L (maximum attainable concentration). OECD 403. Reliability = 1
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- Acceptable, well documented publication/ study report which meets basic scientific principles.
- Principles of method if other than guideline:
- The LD50 was determined after a 24-hour fast with free access to tap-water.
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 205-220 g
- Housing: each housed in individual cages
- Diet: commercial standard solid diet (Altromin-R) ad libitum
- Water: ad libitum - Route of administration:
- oral: unspecified
- Doses:
- 840-1214 mg/kg
- No. of animals per sex per dose:
- 2 groups of 40 rats each
- Control animals:
- not specified
- Details on study design:
- The LD50 was determined after a 24-hour fast with free access to tap-water.
- Sex:
- not specified
- Dose descriptor:
- LD50
- Effect level:
- 1 010 mg/kg bw
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- LD50 (rat): 1010 mg/kg
- Executive summary:
The oral LD50 of the test substance was determined after a 24-hour fast with free access to tap-water. The minimal lethal dose was 850 mg/kg. Single doses larger than 1000 mg/kg lead to a comatose state with death occurring within 12 -36 hours with no liver cell alterations observed microscopically. Liver effects were observed in rats dying 6 -11 days after 850 -1000 mg/kg. The LD50 was 1010 mg/kg.
Reference
The minimal lethal dose was 850 mg/kg. After single doses larger than 1000 mg/kg leading to a comatose state with death occurring within 12 -36 h, no liver cell alterations were seen microscopically; livers of rats dying 6 -11 days after 850 -1000 mg/kg showed extensive centrolobular necrosis with inflammatory reactions and appearance of fat ill surviving liver cells.
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 1 010 mg/kg bw
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- yes
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- strain: SPF Wistar/Chbb : THOM ; breeding facility: Dr. K . Thomae GmbH, D-7950 Biberach, FRG)
Mean body weight at the beginning of the study: male animals 285 ± 4.0 g, female animals 188 ± 2 .1 g.
Age at the beginning of the study: approx . 8- 9 weeks
The animals were identified by color marking on the tail.
The animals were offered KLIBA rat/mouse laboratory diet 24-343-4 10 mm pellets, Klingentalmuhle AG, CH-4303 Kaiseraugst, Switzerland, and drinking water ad libitum during the post-exposure observation period.
The animals were kept in fully air-conditioned rooms in which a temperature in the range 20-24°C and relative humidity in the range 30-70% were regulated by means of a central air-conditioning system. - Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- other: unchanged (no vehicle)
- Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- 0.37 mg/L
- No. of animals per sex per dose:
- 5
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 0.37 mg/L air
- Remarks on result:
- other: maximum concentration achievable
- Mortality:
- No mortality was observed during the study.
- Clinical signs:
- other: Clinical signs included wiping of snouts, restlessness, and attempts to escape. All animals were clear of findings by 2 hours after the beginning of exposure.
- Body weight:
- The body weight gain of male rats was not affected. The body weight gain of the female rats was retarded in the second week of the observation period.
- Gross pathology:
- No pathologic findings noted.
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Regarding the results of the study, the LC50 of the test substance exceeds the maximum concentration that could be technically achieved (0.37 mg/L air).
- Executive summary:
The acute inhalation toxicity potential of the test substance was studied in accordance with OECD Guideline 403. Five male and five female rats were exposed to the test substance for 4 hours at a concentration of 0.37 mg/L (maximum attainable concentration). No mortality or gross pathologic findings were noted was observed.
Reference
Cumulated lethality on day |
male |
female |
0 |
0/5 |
0/5 |
1 |
0/5 |
0/5 |
2 |
0/5 |
0/5 |
7 |
0/5 |
0/5 |
14 |
0/5 |
0/5 |
Total at end of the study |
0/5 |
0/5 |
Time after beginning of exposure |
< ¼ h |
¼ h |
½ h |
1 h |
2 h |
3 h |
4 h |
Animals without findings |
10 |
10 |
10 |
||||
Wiping of snouts |
10 |
||||||
Restlessness |
10 |
10 |
10 |
||||
Attempts to escape |
10 |
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Standardized test (BASF-Test) to assess the acute dermal toxicity in rabbits.
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
- Species:
- rabbit
- Strain:
- Vienna White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Breeder: GAUKLER, 6050 Offenbach
Mean weight for - males: 3.0 kg - females: 3.0 kg
The animals had Ssniff K, standard laboratory diet for rabbits and guinea pigs, INTERMAST GMBH, Soest and water ad libitum. - Type of coverage:
- occlusive
- Vehicle:
- water
- Duration of exposure:
- 24 h
- Doses:
- 200 and 400 mg/kg (50% in water)
- No. of animals per sex per dose:
- 3
- Control animals:
- no
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 400 mg/kg bw
- Mortality:
- see table below
- Clinical signs:
- other: Apathy, diarrhoea
- Gross pathology:
- Dead animals: acute dilatation of the heart (right), degeneration; loamy liver with acute dystrophy; sandy kidneys with nephrosis
Killed animals: no substance-related pathological findings - Interpretation of results:
- Category 3 based on GHS criteria
- Conclusions:
- LD50 (rabbit): 400 mg/kg
- Executive summary:
A standardized test (BASF-Test) to assess the acute dermal toxicity in rabbits was performed. Three male and female rabbits per dose were exposed to the test substance under occlusive conditions for 24 hours to doses of 200 and 400 mg/kg (50% in water). The dermal LD50 in rabbits was 400 mg/kg.
Reference
Mortality
Dose mg/kg |
Conc. % |
Animal |
1 h |
24 h |
48 h |
7 days |
14 days |
400 200 |
50 50 |
3 m 3 f 3 m 3 f |
0/3 0/3 0/3 0/3 |
0/3 0/3 0/3 0/3 |
0/3 0/3 0/3 0/3 |
1/3 2/3 0/3 0/3 |
1/3 2/3 |
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 400 mg/kg bw
Additional information
In a study using the standard acute method, several dose levels between 840 and 1214 mg/kg bw of the test substance (purity unknown) were administered once orally to groups of 40 rats. The LD50 was 1010 mg/kg bw. The minimal lethal dose was 850 mg/kg bw. After single doses larger than 1000 mg/kg bw leading to a comatose state with death occurring within 12 -36 h, no liver cell alterations were seen microscopically; livers of rats dying 6 -11 days after 850 -1000 mg/kg bw showed extensive centrolobular necrosis with inflammatory reactions and appearance of fat ill surviving liver cells.
In a limit test according to OECD Guideline 403, five male and female Wistar rats were whole-body exposed for 4 hours to a dynamically produced vapour with an analytical concentration of 0.37 mg/L (purity 100%). This concentration is the maximum concentration that could be achieved technically. There were no mortalities and no necropsy findings observed. Clinical signs (wiping of snouts, restlessness and attempts to escape) were only observed during exposure. The body weight gain of the males was not affected while the body weight gain of the females was retarded in the second week of the observation period. The LC50 of this study is > 0.37 mg/L.
Following a standardized test protocol (BASF test), groups of three male and three female Vienna White rabbits were treated for 24 hours with doses of 200 mg/kg bw or 400 mg/kg bw of the test substance (purity unknown, prepared in 50% water) epicutaneously under occlusive conditions. Three of six animals died in the high dose treatment and 0/6 in the low dose treatment, leading to a LD50 of 400 mg/kg bw. Clinical signs were apathy and diarrhoea. At necropsy, acute dilatation of the heart (right), degeneration, loamy liver with acute dystrophy and sandy kidneys with nephrosis were observed in animals that died; sacrificed animals were found without substance-related findings.
Justification for classification or non-classification
Based on an oral LD50 in rats of 1010 mg/kg, the test substance is classified for acute oral toxicity as Cat 4 (H302: Harmful if swallowed) according to EU Classification, Labelling and Packaging of Substances and Mixtures (CLP) Regulation (EC) No. 1272/2008.
Based on a dermal LD50 in rabbits of 400 mg/kg, the test substance is classified for acute dermal toxicity as Cat 3 (H311: Toxic in contact with skin) according to EU Classification, Labelling and Packaging of Substances and Mixtures (CLP) Regulation (EC) No. 1272/2008.
Based on a 4-hour inhalation LC50 in rats of >0.37 mg/L (highest attainable concentration) the test substance is not classified for acute inhalation toxicity according to EU Classification, Labelling and Packaging of Substances and Mixtures (CLP) Regulation (EC) No. 1272/2008.
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