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EC number: 277-962-5 | CAS number: 74665-14-8
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
NOEC (72h): 50 mg/L
ErC10 (72h) > 100 mg/L
ErC50 (72h) > 100 mg/L
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 50 mg/L
Additional information
This study was performed to assess the inhibitory effects of phenol sulphonated (EC: 277-962-5; CAS: 74665-14-8) sold under the commercial name SELLATAN P, on the growth of alga Pseudokirchneriella subcapitata, under static condition.
Exponentially growing cultures of P.subcapitata were exposed to 0.0 (Control), 6.25, 12.5, 25.0, 50.0 and 100.0 mg test item/L. Algal cultures were assessed for growth by visual cell count at 24, 48, and 72 h.
The stability test of test item in the test media was performed during the method validation (JRF Study N° 228-
2-13-32625), and test item in the test media was stable up to 72 h (>80% of the nominal concentration). The test media was analysed for active ingredient concentration and stability of the test solution at 0 and 72 h during the main study. The active ingredient concentration of test item in the test media was within the acceptable limit (>80% of the nominal concentration).
A concentration-effect relationship was observed and statistically analysed to obtain effect concentrations. The effect concentration with its respective 95% lower and upper confidence limits for the inhibition of growth rate, biomass and yield were given below:
| Based on the nominal concentration (mg/L) | |||||
| Endpoint (% Inhibition) | NOEC | LOEC | EC10 | EC20 | EC50 |
| 0-72 h Growth rate (95% Confidence Intervals) | 50 | 100 | >100 | > 100 | > 100 |
| 0-72 h Biomass (95% Confidence Intervals) | 90.365 (73.790 – 110.662) | > 100 | > 100 | ||
| 0-72 h Yield (95% Confidence Intervals) | 86.298 (63.533 – 117.220) | > 100 | > 100 | ||
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