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EC number: 241-143-0 | CAS number: 17084-13-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016-05-31 to 2016-06-06
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 2011
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- Council Regulation (EC) No. 761/2009/
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Potassium hexafluorophosphate
- EC Number:
- 241-143-0
- EC Name:
- Potassium hexafluorophosphate
- Cas Number:
- 17084-13-8
- Molecular formula:
- F6P.K
- IUPAC Name:
- potassium hexafluoro-λ⁵-phosphanuide
- Test material form:
- solid: crystalline
- Details on test material:
- - State of aggregation: solid
- Other: crystalline powder,
Colour: white
hygroscopic
Constituent 1
- Specific details on test material used for the study:
- Appearance
White crystalline powder
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
nominal 100mg/l
- Sampling method:
The limit test concentration was diluted factor 10 with HPLC water prior to analysis. Before dilution the samples were centrifuged at 3000 upm for 5 minutes at 20°C. The control was used as blank solution.
- Sample storage conditions before analysis:
All original samples were stored at 6 ± 2 °C. Prepared samples were stored at room temperature until analysis, if necessary.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- stock solution of 100 mg/L was freshly prepared with dilution water.
Media preparation: direct addition of the test item and shaking until the test item was completely dissolved.
one limit concentration of 100 nng/L was tested. The limit concentration is based on the results of a preliminary range finding test
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name:
Pseudokirchneriella subcapitata HINDÁK,
- Strain:
SAG 61.81
- Source (laboratory, culture collection):
Sammlung von Algenkulturen (SAG) Pflanzenphysiologisches Institut der Universität Göttingen Nikolausberger Weg 18, D-37073 Göttingen
- Age of inoculum (at test initiation):
A four day old preculture, prepared in dilution water, was used as inoculum.
- Method of cultivation:
Fresh stocks are prepared every month on Z-Agar. Light intensity amounted to 35-70 µE •m-2 •s-1 for 24 hours per day.
ACCLIMATION
- Acclimation period:
- Culturing media and conditions (same as test or not):
Culture medium: Nutrient medium Z according to LÜTTGE etal. (1994).
Test medium: OECD medium
- Any deformed or abnormal cells observed:
not reported
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- no
Test conditions
- Hardness:
- 0.24 mmol Ca+Mg/L (OECD medium)
- Test temperature:
- 22-23 °C, mean 22.5 °C
- pH:
- 100mg/L test item conc.
0h: 8.13; 72h: 9.68
Control: 0h: 8.22; 72h: 9.44 - Dissolved oxygen:
- not reported
- Salinity:
- freshwater was used
- Conductivity:
- not reported
- Nominal and measured concentrations:
- nominal: 100mg/L
measured: 0h: 82.8mg/L, 72h: 105mg/L (mean of 3 replicates each) - Details on test conditions:
- TEST SYSTEM
- Test vessel:
sterile Erlenmeyer flasks, volume: 250 mL, sealed with cotton wool plugs
- Type (delete if not applicable): open
- Material, size, headspace, fill volume:
glas, 100mgl test volume
- Aeration:
no
- Type of flow-through (e.g. peristaltic or proportional diluter):
static
- Renewal rate of test solution (frequency/flow rate):
static
- Initial cells density:
6305/ml (mean)
- Control end cells density:
start: 6305/ml (mean) end 2323516
- No. of organisms per vessel:
-
- No. of vessels per concentration (replicates):
6
- No. of vessels per control (replicates):
6
- No. of vessels per vehicle control (replicates):
no vehicle
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
OECD medium
- Culture medium different from test medium:
Culture medium: Nutrient medium Z according to LÜTTGE etal. (1994).
- Intervals of water quality measurement:
at least start and end of test
OTHER TEST CONDITIONS
- Adjustment of pH:
no (with OECD medium
- Photoperiod:
24h/day light
- Light intensity and quality:
Approximately 4440 to 8880 lux, corresponding to 60 to 120 µE*m-2*s-1
fluorescent tubes, OSRAM L 36 W/865, cool daylight
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: fluorimeter
- Chlorophyll measurement:
cell density was measured daily via Chlorophyll-a-fluorescence, excitation at 436 nm, emission at 685 nm.
Dilution water was used as background signal. No self fluorescence was found in the range finding test at the concentration of 100 mg/L.
- Other:
Microscopic evaluation of the cells at start and end of the incubation period revealed no morphological abnormalities.
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
limit test 100mg/L
- Justification for using less concentrations than requested by guideline:
limit test due to effect <50% inhibition at range finding test
- Range finding study
yes
- Test concentrations:
1, 10, 100 mg/L
- Results used to determine the conditions for the definitive study:
nominal conc. 1 / 10 / 100 mg/L
growth rate inhibition: -2 / 1 / 9 % - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: nominal concentration analytically confirmed
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Remarks:
- the effect of 3% inhibition or growth rate is statistically significant but is considered to be not biologcically significant
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other:
- Remarks:
- nominal concentration analytically confirmed
- Details on results:
- - Exponential growth in the control (for algal test): yes
increase of cell growth: 369 fold (specific growth rate 1.97 day-1
- Observation of abnormalities (for algal test):
no (microscopic observation at start and end)
- Unusual cell shape:
no morphological abnormalities.
- Colour differences:
no
- Flocculation:
no
- Adherence to test vessels:
no
- Aggregation of algal cells:
no
- Other:
agglutination of algae cells: no
- Any stimulation of growth found in any treatment:
no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
no
- Effect concentrations exceeding solubility of substance in test medium:
no - Results with reference substance (positive control):
- - Results with reference substance valid?
yes
(potassium dichromate)
- EC50:
72h-ErC50: 0.460 mg/L (valid range (average ± 3 x SD): 0.780 ± 0.473 mg/L
- Other: - Reported statistics and error estimates:
- Statistical Significance (NOEC/LOEC) - Growth Rate
t-test
Normality Test (Shapiro-Wilk) Passed (P = 0.319)
Equal Variance Test: Passed (P = 0.513)
Group Name N Missing Mean Std Dev SEM
Control 6 0 1.967 0.0507 0.0207
100 mg/L 6 0 1.900 0.0396 0.0161
Difference 0.0663
t = 2.527 with 10 degrees of freedom. (P = 0.030)
95 percent confidence interval for difference of means: 0.00784 to 0.125
The difference in the mean values of the two groups is greater than would be expected by chance; there is a statistically significant difference between the input groups (P = 0.030).
Power of performed test with alpha = 0.050: 0.551
The program states a significant difference for the nominal concentration of 100 mg/L compared to the control. A statistically significant difference was found but an inhibition of 3 % is considered to be not biologically significant.
Any other information on results incl. tables
Cell Densities
Nominal test item [mg/L |
Replicate No. |
0 hours |
Cell density 24 hours |
[cells/mL] |
72 hours |
|
1 |
6305 |
28107 |
298680 |
1919233 |
|
2 |
6305 |
36443 |
365773 |
1870916 |
|
3 |
6305 |
44034 |
385111 |
2085723 |
100 |
4 |
6305 |
31529 |
253881 |
1637841 |
|
5 |
6305 |
41948 |
374433 |
1666143 |
|
6 |
6305 |
46758 |
377579 |
2206546 |
|
Mean |
6305 |
38137 |
342576 |
1897734 |
|
1 |
6305 |
55874 |
491241 |
2612369 |
|
2 |
6305 |
38983 |
358973 |
1796494 |
|
3 |
6305 |
52596 |
443915 |
2254863 |
Control |
4 |
6305 |
45926 |
453241 |
2091249 |
|
5 |
6305 |
50087 |
500329 |
2615639 |
|
6 |
6305 |
49847 |
509384 |
2570479 |
|
Mean |
6305 |
48886 |
459514 |
2323516 |
Evaluation after 72 hours
Statistically significant differences of growth rates and yield compared to
control values are marked(+)‚ not significant differences are marked(-).
Nominal test item [mg/L] |
Replicate |
Growth rate |
Growth [Vo] |
Yield |
Inhibition of [Vo] |
|
1 |
1.91 |
3 |
1912928 |
17 |
|
2 |
1.90 |
4 |
1864611 |
20 |
|
3 |
1.93 |
2 |
2079418 |
10 |
100 |
4 |
1.85 |
6 |
1631536 |
30 |
|
5 |
1.86 |
5 |
1659838 |
28 |
|
6 |
1.95 |
1 |
2200241 |
5 |
|
Mean |
(+)1 1.90 |
3 |
(+) 1891429 |
18 |
|
1 |
2.01 |
|
2606064 |
|
|
2 |
1.88 |
|
1790189 |
|
|
3 |
1.96 |
|
2248558 |
|
Control |
4 |
1.94 |
|
2084944 |
|
|
5 |
2.01 |
|
2609334 |
|
|
6 |
2.00 |
|
2564174 |
|
|
Mean |
1.97 |
|
2317211 |
|
1 = A statistically significant difference was found but an inhibition of 3 % is considered to be not biologically significant
Section-by-Section and Average Specific Growth Rates of the Control Group (0 - 72 hours)
|
Replicate No. |
Specific growth rate [del] |
Mean (0 - 72 |
SD + |
CV |
Mean CV Fol |
|||||
0 - 24 |
24 - 48 |
48 - 72 |
|||||||||
Control |
1 2 3 4 5 6 |
2.18 1.82 2.12 1.99 2.07 2.07 |
2.17 2.32 |
1.67 1.61 1.63 1.53 1.65 1.62 |
2.01 1.88 1.96 1.94 2.01 2.00 |
0.293 0.310 0.290 0.383 0.328 0.357 |
14.6 16.4 14.8 19.8 16.3 17.8 |
16.6 |
|||
|
Mean |
1.97 |
|
||||||||
|
SD ± |
0.05 |
|
||||||||
|
CV [%] |
2.58 |
|
||||||||
SD = Standard deviation CV = Coefficient of variation
Measured Concentrations and Percent of Nominal Concentration
Sampling date |
2016-05-31 Start of the exposure, 0 hours |
2016-06-03 End of the exposure, 72 hours |
||
Start of analysis |
2016-05-31 |
2016-06-03 |
||
Nominal concentration |
PU-2015-627 |
|||
Meas. conc. |
|
Meas. conc. % [mg/L] |
% |
|
100 |
82.8* |
83 |
105 |
105 |
Control |
< LOQ |
< LOQ |
Meas. conc. = measured concentration of the test item, dilution factors taken into account
% = percent of the nominal concentration of the test item
LOG = limit of quantification of theanalytical method(5.00 mg/L of the test item)
* = reanalysed on 2016-06-06 (mean value of 3 replicates
Validity Criteria
Validity Criterium |
Required |
This study |
Increase of the cell growth in the control cultures |
Exponentially > 16-fold |
369 fold (specific growth rate 1.97 day-1) |
Mean coefficients of variation for section-by-section specific growth rates (days 0-1, 1-2 and 2-3) in the control cultures |
<35 % |
16.6 % |
Coefficient of variation of average specific growth rates during the whole test period in replicate control cultures |
<7 % |
2.58 % |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- for details see end of "any other information on results incl. tables"
- Conclusions:
- nominal 72h-ErC50 > 100 mg/L
nominal 72h-NOEC = 100 mg/L - Executive summary:
Potassium hexafluorophosphate was tested according to OECD201, static with Pseudokirchneriella subcapitata in a limit test with analytically confirmed nominal concentration.
A slight but statistically significant effect difference to control was found but an inhibition of 3 % of groth rate is considered to be not biologically significant.
nominal 72h-ErC50 > 100 mg/L
nominal 72h-NOEC = 100 mg/L
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