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EC number: 212-534-3 | CAS number: 824-72-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2019-08-23 - 2019-09-26
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- no acclimation period of 48 h was performed
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Phenylphosphonic dichloride
- EC Number:
- 212-534-3
- EC Name:
- Phenylphosphonic dichloride
- Cas Number:
- 824-72-6
- Molecular formula:
- C6H5Cl2OP
- IUPAC Name:
- phenylphosphonic dichloride
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Name phenylphosphonic dichloride
Batch no. 201 712 01 - 01
Appearance liquid, clear
Composition phenylphosphonic dichloride: > 98 %
CAS No. 824-72-6
EC-No. 212-534-3
Molecular formula C6H5POCI2
Molecular weight 194.98 g/mol
Purity > 98 %
Homogeneity homogeneous
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- The test item was stored in the test facility in a closed vessel at room temperature (16.2 – 23.7 °C) away from humidity, under inert gas, in the dark.
6.1.3 Preparation
The test item is known to react vigorously with water. Therefore, all analyses refer to the reaction product of the test item after contact with water. Due to the reactivity of the test item, during validation the preparation of the stock solution was identified as the crucial step for reproducible concentrations.
A test solution containing 100.0 mg/L test item in dilution water was prepared.
The medium was put into a vessel and the desired amount of test item was pipetted (72.7 µL/L, based on a density of 1.375 mg/µL, stated in the SDS) into the test medium, followed by incubation for 30 min.
Because of the acid character of the test item, the pH of the dilution water was adjusted to 7.8 before filling up to the desired volume. In a non-GPL pre-test, the pH of a test solution with the concentration 100 mg/L was 3.2 and was adjusted to 7.8.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species Daphnia magna
Authority STRAUS
Strain Berlin
Sex female
Age between 0 and 24 hours
Origin Umweltbundesamt Berlin
In-house breeding since 27. September 2007
Selection of the test system was made following the proposal of the guidelines.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 19.1 – 21.0 °C
- pH:
- 8
- Dissolved oxygen:
- 8.4-8.7 mg/l
- Nominal and measured concentrations:
- 100 mg/l nominal
105-113 mg/l measured - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Any other information on results incl. tables
Nominal Concentration in mg/L |
Immobility 24 hours |
Immobility 48 hours |
||||||||
absolute |
in % |
absolute |
in % |
|||||||
Blank control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The following results were determined for the test item phenylphosphonic dichloride (species: Daphnia magna).
48h-NOEC ≥ 100 mg/L (nominal)
48h-LOEC > 100 mg/L (nominal)
24h-EC50 > 100 mg/L (nominal)
48h-EC50 > 100 mg/L (nominal) - Executive summary:
One valid experiment was performed.
The study was performed as a limit test using the nominal concentration of 100 mg/L. For the test concentration and the blank control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted.
None of the animals was immobilised in the blank control or in the test concentration. Therefore, no statistical evaluation was performed.
Potassium dichromate K2Cr2O7(CAS No. 7778-50-9) was used as positive controlin a current reference study to assure that the test conditions are reliable.
At the beginning and at the end of the test, the content of the test item in the test solutions was determined usingHPLC-UV-determination. The concentration determined at the start of the test was 105 % of the nominal concentration. At the end of the test the determined concentration was 113 % of the nominal concentration. Therefore, the determination of the biological results was based on the nominal concentration (see Guidance Doc. No.23 (§176)).
The following results were determined for the test itemphenylphosphonic dichloride(species:Daphnia magna).
48h-NOEC≥100 mg/L (nominal)
48h-LOEC> 100mg/L (nominal)
24h-EC50> 100 mg/L(nominal)
48h-EC50> 100 mg/L(nominal)
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