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EC number: 269-284-3 | CAS number: 68214-04-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1 September 2016 to 22 September 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- not required
- Vehicle:
- no
- Details on test solutions:
- a stock solution of 10000 mg/L was prepared in deionized water and diluted to get the appropriate concentration with 16 mL nutrient solution and water to give 250 mL. Then, 250 mL inoculum was added.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant in D-67435 NW-Lachen-Speyerdorf
- Method of cultivation: aerated and fed with 50 mL synthetic sewage feed /L daily
- storage time: 1 day
- Preparation of inoculum for exposure: filtered and washed 3 times
- Pretreatment: none
- Initial biomass concentration:exp 1: 3.20 g ss/L, exp 2: 2.96 g ss/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- exp 1: 21.3 - 22.1 °C
exp 2: 20.2 - 21.2 °C
Outside the ranges as required in the guidance, but in view of the inhibition found for controls, this has had no effect on the study outcome. - pH:
- 7.4-7.7
- Nominal and measured concentrations:
- exp 1: 10, 100 and 1000 mg/L
exp. 2: 100 and 320 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beaker
- Aeration: continuous
- No. of vessels per concentration (replicates): 5 (exp 1 at 1000 mg/L; exp 2 at 100 and 320 mg/L)
- No. of vessels per control (replicates): 2 replicates before and 2 after measuring positive control and test item, respectively
- No. of vessels per vehicle control (replicates): NA
- No. of vessels per abiotic control (replicates): not included
- Sludge concentration (weight of dry solids per volume): 3.20 and 2.96 for exp 1 and 2 respectively
- Weight of dry solids per volume of reaction mixture per unit of time: 1.60 and 1.48 for exp 1 and 2 respectively
- Nutrients provided for bacteria: pre-test: synthetic sewage feed according to OECD 209
TEST WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Particulate matter: NA
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: NA
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen consumption during a 5 min period (linearity assessed)
TEST CONCENTRATIONS: 100, 30 and 1000 mg/L
- Range finding study: yes at 1, 10, 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study: 6.7-8.3% inhibition of respiration at 1000 mg/L (statistically significant) - Reference substance (positive control):
- yes
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- validity criteria were fulfilled
- Results with reference substance (positive control):
- EC50 8.8 and 8.1 mg/L exp 1 and 2 respectively
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 for inhibition of respiration of bacteria is > 1000 mg/L, the NOEC is 320 mg/L
- Executive summary:
In a study according to OECD 209, sludge was exposed to the substance during 3 hours at 100, 320 and 1000 mg/L. At 1000 mg/L a slight inhibition of the respiration was observed (max ca 8%), while at both other concentrations no effect was seen. Therefore it is concluded that the NOEC is 320 mg/L and the EC50 is >1000 mg/L.
Reference
Oxygen consumption
Concentration in mg/L |
0 (exp 2) |
100 |
320 |
1000 (exp 1) |
Replicates |
6 |
5 |
5 |
5 |
O2consumption in mg/(L*h) |
43.33 |
44.36 |
41.58 |
52.058 |
O2consumption in mg/(L*h) |
41.61 |
45.97 |
42.77 |
51.767 |
O2consumption in mg/(L*h) |
44.12 |
46.38 |
43.93 |
51.544 |
O2consumption in mg/(L*h) |
44.04 |
46.03 |
44.58 |
52.453 |
O2consumption in mg/(L*h) |
49.12 |
46.47 |
43.71 |
51.824 |
O2consumption in mg/(L*h) |
48.64 |
-- |
-- |
-- |
Mean |
45.14 |
45.84 |
43.31 |
51.93 |
Standard Deviation |
3.03 |
0.86 |
1.16 |
0.35 |
Respiration inhibition
Concentration in mg/L |
0 |
100 |
320 |
1000 (exp 1) |
Replicates |
6 |
5 |
5 |
5 |
Percentage inhibition |
0 |
1.7 |
7.9 |
7.4 |
Percentage inhibition |
0 |
-1.8 |
5.2 |
7.9 |
Percentage inhibition |
0 |
-2.7 |
2.7 |
8.3 |
Percentage inhibition |
0 |
-2.0 |
1.3 |
6.7 |
Percentage inhibition |
0 |
-2.9 |
3.2 |
7.8 |
Percentage inhibition |
0 |
-- |
-- |
-- |
Mean |
0 |
-1.54 |
4.06 |
7.62 |
Description of key information
In a study according to OECD 209, sludge was exposed to the substance during 3 hours at 100, 320 and 1000 mg/L. At 1000 mg/L a slight inhibition of the respiration was observed (max ca 8%), while at both other concentrations no effect was seen. Therefore it is concluded that the NOEC is 320 mg/L and the EC50 is >1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 320 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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