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EC number: 304-519-6 | CAS number: 94276-33-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-07-01 to 2017-08-07
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Hydrogen [3-[[1-[anilinocarbonyl]-2-oxopropyl]azo]-2-hydroxy-5-nitrobenzene-1-sulphonato(3-)]hydroxychromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1)
- EC Number:
- 304-519-6
- EC Name:
- Hydrogen [3-[[1-[anilinocarbonyl]-2-oxopropyl]azo]-2-hydroxy-5-nitrobenzene-1-sulphonato(3-)]hydroxychromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1)
- Cas Number:
- 94276-33-2
- Molecular formula:
- C16 H12 Cr N4 O9 S . C11 H25 N O . H
- IUPAC Name:
- hydrogen 3-[(2-ethylhexyl)oxy]propan-1-amine 11-methyl-4-nitro-12-(phenylcarbamoyl)-6-sulfonato-8λ³-oxa-10λ³-oxa-1λ⁴,13-diaza-9-chromatricyclo[7.4.0.0²,⁷]trideca-1(13),2(7),3,5,10-pentaene-9,9,9-tris(ylium)-8,12-diid-9-olate
- Test material form:
- solid
- Details on test material:
- Physical state / Appearance: Solid / orange to brown
Constituent 1
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature, without exposure to light in a tightly sealed container.
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Samples of all fresh test item concentrations and the control at exposure initiation and all 24-hour-old test item concentrations and the control at renewal were determined. Moreover, samples of fresh test item concentrations (filtrate of a loading of 10 mg/L, filtrate of a loading of 100 mg/L, control at renewal) as well as 24-hour-old test item concentrations (filtrate of a loading of 10 mg/L, filtrate of a loading of 100 mg/L, the control at exposure termination) were determined.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test item is a complex mixture which in incompletely soluble in water. The composition of the soluble fraction changes with the loading. Thus the definitive test was performed using test item concentrations: filtrate of a loading of 100 mg/L, filtrate of a loading of 57 mg/L, filtrate of a loading of 32 mg/L, filtrate of a loading of 18 mg/L, filtrate of a loading of 10 mg/L, plus the control. Appropriate amounts of the test item were weighed separately in glass flasks with ISO test water and mixed with an appropriate volume of the ISO test water [SOP/W/7]. The mixtures were heterogeneous (with non-dissolved particles on the surface). The mixtures and the control (500 mL ISO test water) were left for mechanical shaking for 48 hours (90 revolutions per minute, darkness, room temperature). After 48 hours of mechanical shaking, the mixtures were heterogeneous (visible particles on the surface and bottom of the flask). All mixtures and the control were filtrated through a conditioned nitrocellulose membrane filter (HAWG filter, 0.45 µm, Millipore), [SOP/W/37]. Each filtrate was visually homogeneous (without any visible non-dissolved particles) transparent and colored. The test vessels were conditioned with test item concentrations/control before exposure initiation. The test item concentrations were visually homogeneous, transparent and colored directly after preparation and throughout exposure period.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain: STRAUS
- Source: the test organism originated from the standard laboratory culture cultivated at the Institute of Industrial Organic Chemistry, Branch Pszczyna, Department of Ecotoxicology.
- Age of parental stock (mean and range, SD): Daphnia magna Straus (< 24 h old at exposure initiation); not first brood progeny; neonates collected from the laboratory culture
- Feeding during test: No
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 224 mg/L CaCO3
- Test temperature:
- 18.7 – 19.6 ºC
- pH:
- fresh: 7.7 - 7.84
termination: 7.85 - 7.89 - Dissolved oxygen:
- fresh: 8.5 - 8.9 mg/L
termination: 7.7 - 8.3 mg/L - Salinity:
- 46 mg/L NaHCO3
- Conductivity:
- 598 µS/cm
- Nominal and measured concentrations:
- Nominal: 10, 18, 32, 57, and 100 mg/L (loading)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers
- Type: closed
- Material, size, headspace, fill volume: glass ,150 mL, fill volume: 100 mL
- Aeration: No
- Renewal rate of test solution: after 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Composition of ISO water: see "Any other information on materials and methods"
OTHER TEST CONDITIONS
- Photoperiod: (light/dark) (h) 16/8
EFFECT PARAMETERS MEASURED: mobility
RANGE-FINDING STUDY
- Test concentrations: 12, 120 mg/L, 10-fold diluted filtrate of a loading of 12 mg/L
- Results used to determine the conditions for the definitive study: At exposure termination in the test item concentration filtrate of a loading of 120 mg/L, the immobilization of Daphnia magna was 100% - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- 72.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EL10
- Effect conc.:
- 42.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOELR
- Effect conc.:
- 57 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: LOEC (48 h) ≤ 0.32 mg/L
- Limit test: No
- Dose-response test: Yes
- EC10: 24 h 0.33 mg/L
Any other information on results incl. tables
Table 2: Immobilisation, definitive test
Test item concentration |
Number of Daphnia magna |
Number of immobilized Daphnia magna |
Total of immobilized Daphniamagna [%] |
||||||||
24 h |
48 h |
||||||||||
Replicates |
|||||||||||
A |
B |
C |
D |
A |
B |
C |
D |
24 h |
48 h |
||
Control |
20 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
filtrate of a loading of 10 mg/L |
20 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
filtrate of a loading of 18 mg/L |
20 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
filtrate of a loading of 32 mg/L |
20 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
filtrate of a loading of 57 mg/L |
20 |
0 |
0 |
1 |
1 |
1 |
1 |
3 |
2 |
10 |
35 |
filtrate of a loading of 100 mg/L |
20 |
2 |
1 |
0 |
1 |
4 |
4 |
3 |
4 |
20 |
75 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance is acutely harmful for aquatic invertebrates.
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