3,7-bis(diethylamino)phenoxazin-5-ium acetate

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from peer-reviewed journal

Data source

Reference

Reference Type:

publication

Title:

ACUTE EXPOSURE TO RHODAMINE B

Author:

Daniel J. Dire & Joseph A. Wilkinson

Year:

1987

Bibliographic source:

CLINICAL TOXICOLOGY, 2 5 ( 7 ) , 603-607 (1987)

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as below

Principles of method if other than guideline:

Acute inhalation testing was conducted in individuals exposed through rhodamine B aerosols.

GLP compliance:

not specified

Test type:

other: No data

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride

EC Number:

201-383-9

EC Name:

9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride

Cas Number:

81-88-9

Molecular formula:

C28H31N2O3.Cl

IUPAC Name:

9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthenium chloride

Constituent 2

Reference substance name:

Rhodamine B

IUPAC Name:

Rhodamine B

Test material form:

other: Solid

Details on test material:

- Name of test material (as cited in study report): Rhodamine B
- Molecular formula (if other than submission substance): C28-H31-N2-O3.Cl
- Molecular weight (if other than submission substance): 479.017 g/mole
- Substance type: Organic
- Physical state: Solid

Specific details on test material used for the study:

- Name of test material (as cited in study report): Rhodamine B
- Molecular formula (if other than submission substance): C28-H31-N2-O3.Cl
- Molecular weight (if other than submission substance): 479.017 g/mole
- Substance type: Organic
- Physical state: Solid

Test animals

Species:

other: Human

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

Age at study initiation: 16 males of mean age 39 years (25-50) and one female 40 year old.

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

not specified

Vehicle:

not specified

Remark on MMAD/GSD:

not specified

Details on inhalation exposure:

26 minutes exposure to aerosolized Rhodamine B

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

26 min

Remarks on duration:

not specified

Concentrations:

No data available

No. of animals per sex per dose:

17

Control animals:

not specified

Details on study design:

No data available

Statistics:

No data available

Results and discussion

Preliminary study:

not specified

Effect levels

Sex:

male/female

Dose descriptor:

other: EC

Effect level:

4 530 000 other: mg

Based on:

test mat.

Remarks on result:

other: effect on clinicla sign

Mortality:

No data available

Clinical signs:

other: Burning of eyes, Excessive tearing, Nasal burning and etching, pain/Tightness in Chest, Rhinorhea, Cough, Dyspnea, Burniing of throat, Burning/Pruritic skin, Chest Burning, Headache and Nausea was observed in exposed patients.

Body weight:

No data available

Gross pathology:

No data available

Other findings:

not specified

Any other information on results incl. tables

Symptoms	Number o f Patients	Percent
Burning of eyes	14	82%
Excessive tearing	8	47%
Nasal burning	7	41%
Nasal etching	6	35%
Chest pain/Tightness	5	35%
Rhinorhea	5	29%
Cough	5	29%
Dyspnea	5	29%
Burniing of throat	4	24%
Burning/Pruritic skin	4	24%
Chest Burning	2	12%
Headache	1	6%
Nausea	1	6%
No symptoms	1	6%

Applicant's summary and conclusion

Interpretation of results:

practically nontoxic

Conclusions:

The most frequent symptoms were ocular and upper respiratory tract mucous membrane irritation. But all the symptoms resolved in 24 hours .No serious effects observed .Based on this the Effect concentration could be reported as approx (4.53kg) 4530000mg.

Executive summary:

Approximately 10 lbs=4.53kg of Rhodamine B powder was spilled onto the floor of a vehicle maintenance shop. A cleanup crew began to sweep up the powder from the floor . This resulted in an aerosol of Rhodamine B dust that dispersed throughout the building contaminating the hair , skin, eyes, and clothing of most of the shop employees. 16 out of 17 of the patients (94%) complained of symptoms which are - One patient (6%) denied any symptoms despite being exposed to the dust for 15 minutes without a surgical mask. The most frequently reported symptom was burning of the eyes which occurred in 83% of the patients . Four of the patients (24%) were placed on humidified oxygen because of respiratory symptoms. Five o f the patients (29%) underwent irrigation of their eyes secondary to eye burning. After being discharged from the hospital and follow up is maintained . Ten of the patients (59%) reported that their symptoms had resolved within 4 hours of their exposure to the dust. One patient (6%) reported that his symptoms lasted 12 hours and 2 patients (12%) reported their symptoms lasted 24 hours. Three of the patients (18%) could not remember the duration of their symptoms. No serious effects observed .Based on this the Effect concentration could be reported as approx (4.53kg) 4530000mg.