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Diss Factsheets
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EC number: 200-806-4 | CAS number: 74-31-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Specific investigations: other studies
Administrative data
- Endpoint:
- specific investigations: other studies
- Remarks:
- embryotoxicity
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Referenceopen allclose all
- Reference Type:
- secondary source
- Title:
- Embryotoxicity of Sixteen Industrial Amines to the Chicken Embryo
- Author:
- Korhonen A. et al.
- Year:
- 1 983
- Bibliographic source:
- J. Appl. Toxicol., 3, 112-117, (1983)
- Reference Type:
- publication
- Title:
- Toxicity of rubber chemicals towards three-day chicken embryos
- Author:
- Korhonen A. et al.
- Year:
- 1 983
- Bibliographic source:
- Scand. j. work. environ. health, 9,115-119, (1983)
Materials and methods
- Principles of method if other than guideline:
- A method utilizing chicken embryos is employed to allow observation of each exposed embryo throughout the whole period of embryonic development. The advantage of continuous observation is that dead embryos can be readily recorded and inspected before maceration.
- GLP compliance:
- not specified
- Type of method:
- other: in ovo
- Endpoint addressed:
- developmental toxicity / teratogenicity
Test material
- Reference substance name:
- N,N'-diphenyl-p-phenylenediamine
- EC Number:
- 200-806-4
- EC Name:
- N,N'-diphenyl-p-phenylenediamine
- Cas Number:
- 74-31-7
- Molecular formula:
- C18H16N2
- IUPAC Name:
- N,N'-diphenyl-p-phenylenediamine
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source of test material: Oy Nokia Ab, Nokia
- Purity test date: technical grade
Test animals
- Species:
- other: chicken
- Strain:
- other: White Leghorn chicken egg
- Details on test animals or test system and environmental conditions:
- - Source: hatchery of Siipikarjanhoitajain Liito ry, Hämeenlinna, Finland
Administration / exposure
- Route of administration:
- other: injection
- Vehicle:
- acetone
- Details on exposure:
- VEHICLE
- Purity: p.a. grade, from Merck
Doses / concentrations
- Dose / conc.:
- 3 other: µmol per egg
- Remarks:
- Equals highest feasible dose based on solubility or suspendibility in 5 µL of acetone
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- CONTROLS
- The solution containing test compound was injected into the egg in a total volume of 5 µL. Ten control eggs, injected with 5 µL of acetone, were incubated with each batch of eggs. Results from solvent controls were not subtracted from experimental values. The background level of 1-2% was insignificant against the variation of the method.
EXPERIMENTAL DETAILS
- Three-day (72-76 hours) embryos were selected by candling.
- The test compound is injected on the inner shell membrane, focusing the solution drop exactly on the embryo.
- Two days after the injection, the eggs were candled again. Eggs containing dead embryos were counted and discarded.
- The remaining eggs were then candled every second or third day. Those containing dead embryos were opened and the embryos checked for external malformations and the developmental stage.
- The incubation was terminated 11 days after the injection, after a total incubation of 14 days. The remaining eggs were opened and the embryos inspected for survival and for external malformations.
- The temperature was kept at 37.7 “C and the humidity between 66% and 71% throughout the incubation period. The eggs were turned two to four times per day.
CLASSIFICATION
The affected embryos were classified into the following categories:
- Early deaths, embryos that died before day 5 of the incubation, within two days of the treatment.
- Late deaths, non-malformed, externally normal embryos that died between days 5 and 14.
- Late deaths, malformed, externally malformed embryos that died between days 5 and 14.
- Malformed survivors, externally malformed embryos alive on day 14 of the incubation.
LD50 and ED50 values were calculated using a Wang table computer.
Results and discussion
- Details on results:
- The test compound dit not produce effects above the vehicle background.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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