Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-423-0 | CAS number: 106-68-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 Apr 2017 - 12 May 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Justification for type of information:
- Information is used for read across to Ethyl amyl ketone (EAK)
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- ISO 10707 Water quality - Evaluation in an aqueous medium of the "ultimate" aerobic biodegradability of organic compounds - Method by analysis of biochemical oxygen demand (closed bottle test)
- Version / remarks:
- 1994
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: EM97160108
- Expiration date of the lot/batch: 14 January 2018 (expiry date)
- Purity test date: 07/07/2016 (certificate of analysis)
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At room temperature
- Solubility and stability of the test substance in the solvent/vehicle: Solubility in Water: 167.3 mg/L (20ºC, pH=6.3)
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: n/a
- Preliminary purification step (if any): n/a
- Final preparation of a solid: n/a
OTHER SPECIFICS:
Purity/composition correction factor: No correction factor required
Test item handling: No specific handling conditions required
Molecular formula: C9H18O
Molecular weight: 142.239
Stability in Water: Not available - Oxygen conditions:
- aerobic
- Inoculum or test system:
- natural water
- Details on inoculum:
- Source:
The source of test organisms was secondary effluent freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
Treatment:
Secondary effluent was filtered through a coarse filter paper, the first 200 mL was discarded. The filtrate was kept aerated until inoculation.
Inoculation:
4 mL filtrate of secondary effluent per litre of final volume - Duration of test (contact time):
- 28 d
- Initial conc.:
- 1 mg/L
- Based on:
- test mat.
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Remarks:
- as a percentage of ThOD
- Details on study design:
- TEST CONDITIONS
-Test duration:
28 days for the inoculum blank and test suspension; 14 days for the procedure and toxicity control
- Milli-RO water:
Tap-water purified by reverse osmosis (Millipore Corp., Bedford, Mass., USA).
- Stock solutions of mineral components:
A)8.50 g KH2PO4
21.75 g K2HPO4
67.20 g Na2HPO4.12H2O
0.50 g NH4Cl
dissolved in 1 litre Milli-RO water, pH 7.4 + 0.2
B)22.50 g MgSO4.7H2O dissolved in 1 litre Milli-ROwater.
C)36.40 g CaCl2.2H2O dissolved in 1 litre Milli-RO water.
D)0.25 g FeCl3.6H2O dissolved in 1 litre Milli-RO water.
-Mineral medium:
1 mL of solution (A) to (D) was mixed and made up to 1 litre with Milli-RO water.
The concentration of dissolved oxygen was measured for control purposes. The mineral medium was left at test temperature to obtain a saturated solution at the start of the test.
- Test temperature: 22.0 - 23.1°C.
- pH: 7.6
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Test bottles: 250-300 mL BOD bottles with glass stoppers.
- Number of culture flasks/concentration: Individual BOD bottles were prepared for each measuring bottles point, except that the bottles measured at the start were also measured on day 7. Parallel groups were prepared to allow duplicate measurements of oxygen consumption at the test intervals.
- Measuring equipment: WTW oxygen meter supplied with a WTW CellOx 325 oxygen
electrode, electrolyte type ELY/G.
- Test performed in closed vessels due to significant volatility of test substance: yes
SAMPLING
- Sampling frequency: In duplicate; immediately at the start of the experiment (day 0), and at day 7, 14, and on day 21 and 28 for the inoculum blank and test item.
Determination of oxygen concentration:
Oxygen meter: WTW oxygen meter supplied with a WTW CellOx 325 oxygen
electrode, electrolyte type ELY/G.
pH: At the start of the test.
Temperature of medium: Continuously in a vessel with Milli-RO water in the same room.
CONTROL AND BLANK SYSTEM
Inoculum blank: Containing only inoculum (no test item)
Procedure control: Containing reference item and inoculum
Test suspension: Containing test item and inoculum (applicable for both test concentrations)
Toxicity control: Containing test item at the lowest concentration, reference item and inoculum.
ANALYSIS:
The specific theoretical oxygen demand (ThOD) of the test item was calculated, since the
elemental composition of the test item was known. - Reference substance:
- acetic acid, sodium salt
- Test performance:
- The test is valid as shown by an endogenous respiration of 1.0 mg/L at day 28. Furthermore, the differences between the replicate values at day 28 were less than 20% (≤ 11%). Sodium acetate was degraded by at least 60% (76%) of its theoretical oxygen demand within 14 days. Degradation of sodium acetate was not inhibited by the test substance. Finally, the concentration of dissolved oxygen did not fall below 0.5 mg/L in all bottles during the test period.
As for test conditions, the pH values at the start of the test were 7.6 for all vessels. The temperature recorded in a vessel with water in the same room varied between 22.0 and 23.1°C. - Parameter:
- % degradation (O2 consumption)
- Value:
- 52
- Sampling time:
- 7 d
- Remarks on result:
- other: 1mg/L dose
- Parameter:
- % degradation (O2 consumption)
- Value:
- 66
- Sampling time:
- 14 d
- Remarks on result:
- other: 1mg/L dose
- Parameter:
- % degradation (O2 consumption)
- Value:
- 63
- Sampling time:
- 21 d
- Remarks on result:
- other: 1mg/L dose
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 60
- Sampling time:
- 28 d
- Remarks on result:
- other: 1mg/L dose
- Parameter:
- % degradation (O2 consumption)
- Value:
- 62
- Sampling time:
- 7 d
- Remarks on result:
- other: 2 mg/L dose
- Parameter:
- % degradation (O2 consumption)
- Value:
- 65
- Sampling time:
- 14 d
- Remarks on result:
- other: 2 mg/L dose
- Parameter:
- % degradation (O2 consumption)
- Value:
- 62
- Sampling time:
- 21 d
- Remarks on result:
- other: 2 mg/L dose
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 57
- Sampling time:
- 28 d
- Remarks on result:
- other: 2 mg/L dose
- Details on results:
- The relative biodegradation values calculated from the O2 measurements performed during the test period of 28 days revealed significant biodegradation of Nonan-2-one (methyl heptyl ketone) at both concentrations at the end of the test, i.e. 60% and 57% for the low and high concentration, respectively. In addition, >60% biodegradation was attained within a 14-day window.
Apparently, the total biodegradation of the test item slightly decreases from day 14 to day 28. This is due to experimental variation in the individual inoculum blanks as well as the test item replicates. The fact that the test item was >60% biodegraded after 7 and 14 days of incubation in the high and low concentration, respectively, firmly supports a readily biodegradable classification. - Results with reference substance:
- Sodium acetate (procedure control) was degraded by at least 60% (76%) within 14 days which fulfills validity criteria
- Validity criteria fulfilled:
- yes
- Remarks:
- The reference substance, toxicity of the test substance, the difference of the replicates at 28d, O2 depletion (inoculum blank) and residual O2 conc in the test bottles met the validity criteria.
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Nonan-2-one (methyl heptyl ketone) showed >=60% biodegradation in an OECD TG 301D test at 28d and met the 14-day time window so is considered to be readily biodegradable.
- Executive summary:
The ready biodegradability of Nonan-2-one (methyl heptyl ketone) was investigated in a study conducted in accordance with OECD TG 301D (closed bottle test) and GLP. Nominal concentrations of 1 and 2 mg/L of Nonan-2-one (methyl heptyl ketone) were tested. Measurements of the oxygen concentrations were performed at the start of the experiment (day 0) and at day 7, 14, and on day 21 and 28 for the inoculum blank and test item. Sodium acetate was degraded by at least 60% (76%) of its theoretical oxygen demand within 14 days. In addition the toxicity control showed that the substance did not inhibit microbial activity. The relative biodegradation values calculated from the O2 measurements performed during the test period of 28 days revealed significant biodegradation of Nonan-2-one (methyl heptyl ketone) at both concentrations at the end of the test, i.e. 60% and 57% for the low and high concentration, respectively. 60% biodegradation of Nonan-2-one (methyl heptyl ketone) was already reached after 7 and 14 days of incubation in the high and low concentration, respectively. Nonan-2-one (methyl heptyl ketone) is therefore designated as readily biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- See attached justification document
- Reason / purpose for cross-reference:
- read-across source
- Test performance:
- The test is valid as shown by an endogenous respiration of 1.0 mg/L at day 28. Furthermore, the differences between the replicate values at day 28 were less than 20% (≤ 11%). Sodium acetate was degraded by at least 60% (76%) of its theoretical oxygen demand within 14 days. Degradation of sodium acetate was not inhibited by the test substance. Finally, the concentration of dissolved oxygen did not fall below 0.5 mg/L in all bottles during the test period.
As for test conditions, the pH values at the start of the test were 7.6 for all vessels. The temperature recorded in a vessel with water in the same room varied between 22.0 and 23.1°C. - Parameter:
- % degradation (O2 consumption)
- Value:
- 52
- Sampling time:
- 7 d
- Remarks on result:
- other: 1mg/L dose
- Parameter:
- % degradation (O2 consumption)
- Value:
- 66
- Sampling time:
- 14 d
- Remarks on result:
- other: 1mg/L dose
- Parameter:
- % degradation (O2 consumption)
- Value:
- 63
- Sampling time:
- 21 d
- Remarks on result:
- other: 1mg/L dose
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 60
- Sampling time:
- 28 d
- Remarks on result:
- other: 1mg/L dose
- Parameter:
- % degradation (O2 consumption)
- Value:
- 62
- Sampling time:
- 7 d
- Remarks on result:
- other: 2 mg/L dose
- Parameter:
- % degradation (O2 consumption)
- Value:
- 65
- Sampling time:
- 14 d
- Remarks on result:
- other: 2 mg/L dose
- Parameter:
- % degradation (O2 consumption)
- Value:
- 62
- Sampling time:
- 21 d
- Remarks on result:
- other: 2 mg/L dose
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 57
- Sampling time:
- 28 d
- Remarks on result:
- other: 2 mg/L dose
- Details on results:
- The relative biodegradation values calculated from the O2 measurements performed during the test period of 28 days revealed significant biodegradation of Nonan-2-one (methyl heptyl ketone) at both concentrations at the end of the test, i.e. 60% and 57% for the low and high concentration, respectively. In addition, >60% biodegradation was attained within a 14-day window.
Apparently, the total biodegradation of the test item slightly decreases from day 14 to day 28. This is due to experimental variation in the individual inoculum blanks as well as the test item replicates. The fact that the test item was >60% biodegraded after 7 and 14 days of incubation in the high and low concentration, respectively, firmly supports a readily biodegradable classification. - Results with reference substance:
- Sodium acetate (procedure control) was degraded by at least 60% (76%) within 14 days which fulfills validity criteria
- Validity criteria fulfilled:
- yes
- Remarks:
- Results for EAK are validated in the read across justification
- Interpretation of results:
- readily biodegradable
- Conclusions:
- According to the read-across assessment from the source Nonan-2-one (MHK) to the target substance Octan-3-one (EAK), EAK is readily biodegradable.
Nonan-2-one (methyl heptyl ketone) showed >=60% biodegradation in an OECD TG 301D test at 28d and met the 14-day time window so is considered to be readily biodegradable. - Executive summary:
Based on the read across assessment from the source Nonan-2-one (MHK) to the target substance Octan-3-one (EAK), EAK is considered readily biodegradable.The ready biodegradability of Nonan-2-one (methyl heptyl ketone) was investigated in a study conducted in accordance with OECD TG 301D (closed bottle test) and GLP. Nominal concentrations of 1 and 2 mg/L of Nonan-2-one (methyl heptyl ketone) were tested. Measurements of the oxygen concentrations were performed at the start of the experiment (day 0) and at day 7, 14, and on day 21 and 28 for the inoculum blank and test item. Sodium acetate was degraded by at least 60% (76%) of its theoretical oxygen demand within 14 days. In addition the toxicity control showed that the substance did not inhibit microbial activity. The relative biodegradation values calculated from the O2 measurements performed during the test period of 28 days revealed significant biodegradation of Nonan-2-one (methyl heptyl ketone) at both concentrations at the end of the test, i.e. 60% and 57% for the low and high concentration, respectively. 60% biodegradation of Nonan-2-one (methyl heptyl ketone) was already reached after 7 and 14 days of incubation in the high and low concentration, respectively. Nonan-2-one (methyl heptyl ketone) is therefore designated as readily biodegradable.
Referenceopen allclose all
In the toxicity control more than 25% biodegradation occurred within 14 days (71%, based on ThOD). Thus, the toxicity control showed that Nonan-2-one (methyl heptyl ketone) did not inhibit microbial activity.
In the toxicity control more than 25% biodegradation occurred within 14 days (71%, based on ThOD). Thus, the toxicity control showed that Nonan-2-one (methyl heptyl ketone) did not inhibit microbial activity.
Description of key information
Based on the read across assessment from the source Nonan-2-one (MHK) to the target substance Octan-3-one (EAK), EAK is considered readily biodegradable, based on a OECDTG301D test result, >= 60% biodeg at 28d.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Read across justification is attached in the Target file.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.