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EC number: 812-037-7 | CAS number: 1793011-72-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Reaction products of diazotized 4-amino-6-[(4-aminophenyl)diazenyl]-3-[(4-aminophenyl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid, coupled with benzene-1,3-diamine, sodium salts
- EC Number:
- 812-037-7
- Cas Number:
- 1793011-72-9
- Molecular formula:
- Unknown for all components
- IUPAC Name:
- Reaction products of diazotized 4-amino-6-[(4-aminophenyl)diazenyl]-3-[(4-aminophenyl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid, coupled with benzene-1,3-diamine, sodium salts
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 1.0, 3.2, 10, 32 and 100 mg/L (nominal)
- Sampling method: Samples were taken at 0 and 72 hours after exposure at each concentration and analysed by HPLC.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A stock solution of 100 mg/L was prepared by adding the appropriate amount of substance to 1 L of test media in a Mess flask. This was stirred for 5 minutes. This stock solution was then diluted as appropriate to provide the remaining test concentrations (50 mL test solutions in 500 mL erlenmeyer flasks with aluminium caps).
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella Subcapitata
- Strain: ATCC22662
- Source (laboratory, culture collection): American Type Culture Collection
- Method of cultivation: Maintained by periodic subculture using Gorham medium. Algae are kept under sterile conditions.
ACCLIMATION
- Acclimation period: 4 days
- Any deformed or abnormal cells observed: No
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- Not specified
- Test temperature:
- 21.3 to 22.1°C throughout the test
- pH:
- 7.8 to 7.9 throughout the test
- Dissolved oxygen:
- Not specified
- Salinity:
- Not specified
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Nominal: 1.0, 3.2, 10, 32 and 100 mg/L
Measured (mean): 0.961, 3.15, 9.92, 30.5 and 102 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL Erlenmeyer flask with aluminium cap
- Type: open (although capped with aluminium)
- Material, size, headspace, fill volume: 50 mL
- Aeration: No
- Initial cells density: 5 x 10^3
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes (OECD medium as detailed in the respective guideline)
OTHER TEST CONDITIONS
- Sterile test conditions: Yes
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 110 to 120 µE/m2/s
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: cells/mL (biomass) and growth rate
TEST CONCENTRATIONS
- Range finding study: First test (100 mL/vessel) Control, 1.0, 10 and 100 mg/L. Second test (50 mL/vessel) Control, 1, 5, 10 and 100 mg/L.
- Test concentrations: 1.0, 3.2, 10, 32 and 100 mg/L
- Results used to determine the conditions for the definitive study: As the substance is black and water soluble the range-finder was conducted at an increased light intensity with test volumes of 50 or 100 mL. The results showed high toxicity at 100 mg/L in both experiments which decreased through the concentrations. It was determined that the growth inhibition of algae was indeed affected by the attenuation of light as a result of the test substance colour. The inhibitory effect was less pronounced when using test solution volumes of 50 mL and accordingly this was used in the main test. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 29.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: effect likely caused by the attenuation of light from the coloured substance rather than a true toxic effect
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: effect likely caused by the attenuation of light from the coloured substance rather than a true toxic effect
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): No
- Unusual cell shape: No
- Colour differences: Control vessels were colourless, 1 and 3.2 mg/L were indigo and 10, 32 and 100 mg/L were black.
- Flocculation: No
- Adherence to test vessels: No
- Aggregation of algal cells: No
- Any stimulation of growth found in any treatment: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- Growth inhibition tests with a reference reagent potassium dichromate are conducted every 6 months. The ErC50 values obtained in the most recent test were within historical ranges for tests conducted at the laboratory.
- Reported statistics and error estimates:
- The EC50 was determined using the least squares linear regression analysis of points recognised as straight in the concentration-inhibition curves. 95% confidence limits were calculated where possible. The NOEC was determined by an analysis of variance (ANOVA), Williams test, subsequent to Bartlett test for homogeniety of variance.
Any other information on results incl. tables
Biomass in the control cultures increased exponentially above 16 fold during the 72 hour culture. All validity criteria with regards to the controls were met.
The ability of the substance to affect light transmission was seperately investigated. This showed that light transmission was greatly reduced where the test substance was used as a filter causing high increases in growth inhibition. This showed that the toxicity observed in the definitive phase was likely to be as a result of the substance's ability to block light transmission to algal cells.
Growth inhibitions (%) of Pseudokirchneriella subcapitata
Test group |
Nominal [Mean measured] mg/L |
Vessel No. |
Growth rate |
|
Rate µ (0-72 hr) |
Inhibition (%) Iµ ( 0-72 hr) |
|||
Control |
NA |
1 |
0.0720 |
NA |
2 |
0.0694 |
|||
3 |
0.0739 |
|||
4 |
0.0722 |
|||
5 |
0.0692 |
|||
6 |
0.0712 |
|||
Average |
0.0713 |
NA |
||
SD |
0.0018 |
|||
Conc. 1 |
1.0 [0.61] |
1 |
0.0754 |
-5.8 |
2 |
0.0747 |
-4.8 |
||
3 |
0.0717 |
-0.6 |
||
Average |
0.0739 |
-3.7 |
||
SD |
0.0020 |
|||
Conc. 2 |
3.2 [3.15] |
1 |
0.0658 |
7.7 |
2 |
0.0720 |
-1.0 |
||
3 |
0.0692 |
2.9 |
||
Average |
0.0690 |
3.2 |
||
SD |
0.0031 |
|||
Conc. 3 |
10 [9.92] |
1 |
0.0565 |
20.8 |
2 |
0.0604 |
15.3 |
||
3 |
0.0549 |
23 |
||
Average |
0.0573** |
19.7 |
||
SD |
0.0028 |
|||
Conc. 4 |
32 [30.5] |
1 |
0.0342 |
52 |
2 |
0.0349 |
51.1 |
||
3 |
0.0360 |
49.5 |
||
Average |
0.0350 |
50.9 |
||
SD |
0.0009 |
|||
Conc. 5 |
100 [102] |
1 |
0.0104 |
85.4 |
2 |
0.0096 |
86.5 |
||
3 |
0.0116 |
83.7 |
||
Average |
0.0105** |
85.2 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Validity criteria on test fulfilled
- Conclusions:
- The 72 hour ErC50 of the test substance was determined to be 29.3 mg/L with the corresponding NOEC as 3.15 mg/L. However, the effects observed were most likely a result of the substance's properties (black coloured) which attenuated light minimising the potential for photosynthesis of the algae.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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