Cobalt zinc silicate blue phenacite

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

adopted 1992-07-17

Deviations:

yes

Principles of method if other than guideline:

Only nominal concentration given; no data about measured test substance concentration is given (in eluats); only a brief description of the test conditions is given.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Cobalt zinc silicate blue phenacite

EC Number:

270-208-6

EC Name:

Cobalt zinc silicate blue phenacite

Cas Number:

68412-74-8

Molecular formula:

Zn(2-x)Co(x)SiO4 0,7≤x≤1,1

IUPAC Name:

cobalt zinc silicate blue phenacite

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): "Farbkörper DUNKELBLAU 226579"
- Physical state: dark blue powder
- Storage condition of test material: room temperature

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not applicable

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

no data

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method:
The test substance is hardly soluble. Therefore, before starting the test, the test substance (10000 mg test item) was suspended in dilution water (drinking water) and placed in a mechnical shaker (approximately 130 rpm) for 24 hours. After this time period of incubation the suspension was filtered (fluted filter; rinsed with deionised water before filtration was conducted). The clear filtrate (eluate) was used in the test without further dilution.
- Controls: blank control (assumed, not clearly stated in the study report)

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:
- Strain: Brachydanio rerio
- Source: Tagis-Aquarium, Dreieich

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

no data

Test temperature:

19.5-23.0°C

pH:

Control: 8.07-8.61
Test vessel: 7.62-8.7

Dissolved oxygen:

Control: 8.1-8.4 mg O2/L
Test vessel: 8.1-8.3 mg O2/L

Salinity:

not applicable

Nominal and measured concentrations:

Nominal concentration: 10000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: one vessel with 12 litre
- Aeration: yes
- No. of organisms per vessel: 7
- No. of vessels per control (replicates): no data
- Biomass loading rate: <1g Fish/L

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h dark/ 8 h light

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 10 000 other: mg/L water accomodated fraction

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 other: mg/L water accomodated fraction

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 10 000 other: mg/L water accomodated fraction

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

no data

Any other information on results incl. tables

Sublethal observations / clinical signs:

Limit test

Concentration of test item [mg/L]	Mortality [%]
	24h	48h	72h	96h
Control	0	0	0	0
Eluate 10000	0	0	0	0

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

Acute toxic effects could not be observed
The 96h-LC50 for cobalt zinc silicate blue phenacite toxicity (survival) to Brachydanio rerio is >10,000 mg/L (water accomodated fraction). This value will further be used in the chemical safety assessment. The corresponding LC0 is ≥ 10,000 mg/L (water accomodated fraction) as well.