Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 944-041-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- other: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- Source study has reliability 2, due to low purity of test sample. Details on the read across are available in section 13.
- Qualifier:
- according to guideline
- Guideline:
- other: guideline 79/831/EEC, Annex V, part C
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: a stock solution (200 mg/l) was prepared by dissolving the substance in deionised water - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: CHODAT SAG 86.81
- Source (laboratory, culture collection): obtained from collection of algal cultures in Göttingen, Germany - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23 ± 2° C
- pH:
- 7.40 - 9.38
- Nominal and measured concentrations:
- Nominal test substance concentrations:
0 (control), 0.16, 0.31, 0.63, 1.25, 2.5, 5, 10, 20 and 100 mg/l
Analytically determined concentrations:
10.2 mg/l (10 mg/l nominal)
1.23 mg/l (1.25 mg/l nominal) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flask (250 ml)
- Fill volume: 100 ml
- Initial cells density: 10000 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised
OTHER TEST CONDITIONS
- Photoperiod: continous illumination
- Light intensity and quality: 120 μEinstein/m*m*s, Color of light is universal white, L 25 (Osram)
EFFECT PARAMETERS MEASURED
- Determination of cell concentrations: electronic particle counter
- Chlorophyll measurement: light flashes, wavelength 435 nm - Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 10.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 71.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.19 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 18.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate and biomass
- Conclusions:
- EC50 (72h) = 71.8 mg/l based on growth rate.
- Executive summary:
Method:
Toxicity to algae was assessed following EEC 79/831 Annex V part C. Nominal test substance concentrations were 0 (control), 0.16, 0.31, 0.63, 1.25, 2.5, 5, 10, 20 and 100 mg/l. For each concentration, 3 replicates were tested. Effects on growth rate and biomass were assessed 0, 24, 48 and 72 h after start.
Results:
Effects on growth rate after 72 h:
EC10 = 10.3 mg/l
EC50 = 71.8 mg/l
EC90 > 100 mg/l
Effects on biomass after 72 h:
EC10 = 0.19 mg/l
EC50 = 18.4 mg/l
EC90 > 100 mg/l
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- other: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- Source study has reliability 2, due to low purity of test sample. Details on the read across are available in section 13.
- Qualifier:
- according to guideline
- Guideline:
- other: guideline 79/831/EEC, Annex V, part C
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: a stock solution (400 mg/l) was prepared by dissolving the substance in deionised water. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: CHODAT SAG 86 .81
- Source (laboratory, culture collection): obtained from collection of algal cultures in Göttingen, Germany - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23 ± 2°C
- pH:
- 7.91 - 9.72
- Nominal and measured concentrations:
- Nominal test substance concentrations:
control, 0.16, 0.31, 0.63, 1.25, 2.5, 5, 10, 20 and 100 mg/l
Analytically determined concentrations:
0.17 mg/l (0.078 mg/l nominal)
19.4 mg/l (20 mg/l nominal)
397 mg/l (400 mg/l nominal) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flask (250 ml)
- Fill volume: 100 ml
- Initial cells density: 10000 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised
OTHER TEST CONDITIONS
- Photoperiod: continous illumination
- Light intensity and quality: 120 μEinstein/m*m*s, Color of light is universal white, L 25 (Osram)
EFFECT PARAMETERS MEASURED
- Determination of cell concentrations: electronic particle counter
- Chlorophyll measurement: light flashes, wavelength 435 nm - Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 21.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 80.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2.73 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 32.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate and biomass
- Conclusions:
- EC50 (72h) = 80.8 mg/l based on growth rate.
- Executive summary:
Method:
Toxicity to algae was assessed following EEC 79/831 Annex V part C. Nominal test substance concentrations were 0 (control), 0.16, 0.31, 0.63, 1.25, 2.5, 5, 10, 20 and 100 mg/l. For each concentration, 3 replicates were tested. Effects on growth rate and biomass were assessed 0, 24, 48 and 72 h after start.
Results:
Effects on growth rate after 72 h:
EC10 = 21.8 mg/l
EC50 = 80.8 mg/l
EC90 > 100 mg/l
Effects on biomass after 72 h:
EC10 = 2.73 mg/l
EC50 = 32.9 mg/l
EC90 > 100 mg/l
Referenceopen allclose all
Description of key information
EC50 (72h) algae = 71.8 mg/l (nominal)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 71.8 mg/L
Additional information
No data on target substance was available, thus a read across approach was followed, using data on Similar Substance 01. Details are reported in section 13.
Toxicity to aquatic algae was assessed in 2 studies on Similar Substance 01, under static exposure conditions and exposure duration of 72 h. Both studies were well-documented and reliable, thus the study reporting the lowest EC50 value was selected as key study by following a precautionary approach.
In both studies, toxicity to algae was assessed according to guideline 79/831/EEC Annex V, part C; nominal concentrations on Similar Substance 01 were 0, 0.16, 0.31, 0.63, 1.25, 2.5, 5, 10, 20, 100 mg/l, divided in 3 replicates per concentration; effects on growth rate and biomass were recorded 0, 24, 48 and 72 h after start of exposure.
After 72 hour, EC50 values of 71.8 and 80.8 mg/l based on growth rate were found.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.