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EC number: 268-974-1 | CAS number: 68155-62-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- From October 31st to November 28th, 1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Justification for type of information:
- The read across approach is detailed into the document attached to the IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- Version / remarks:
- adopted July 17, 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-B (Determination of the "Ready" Biodegradability - Modified OECD Screening Test)
- Version / remarks:
- December 1992
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Type: Microorganisms from a domestic waste water treatment plant.
- Source: ARA Ergolz ll, Füllinsdorf / Switzerland.
- Pretreatment: the activated sludge was washed three times with tap water and an amount corresponding to 4 g dry material (± 10 %) per litre was mixed with Sörensen buffer solution pH 7 and was then aerated until use.
- Preparation of the inoculum: in amount of 0.5 ml sludge (filtered through cotton wool) was added to 1000 ml test medium (with the exception of the abiotic control). - Duration of test (contact time):
- 28 d
- Initial conc.:
- 32.9 - 34.1 mg/L
- Based on:
- DOC
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: 50 ml Erlenmeyer flasks, cleaned with alcoholic hydrochloric acid, rinsed with bidistilled water and dried. The test vessels were filled with about 30 ml of test medium, or test medium containing test article and/or reference compound.
- Additional substrate:
- Solubilising agent (type and concentration if used):
- Test temperature: 21 - 22 °C
- Continuous darkness: the test vessels were protected from light by means of a loose cover with aluminium foil.
- Agitatin: shaking water bath.
MEDIUM
The test medium (30 ml per flask) was prepared according to the OECD Guideline No. 301E.
CONTROL AND BLANK SYSTEM
- Inoculum blank: untreated test medium was inoculated and used as the control.
- Abiotic sterile control: the concentration of the test article in sterile filtered test medium (0.45 µm filtered) was 100 mg/I, corresponding to an actual amount of 33.8 mg DOC/l.
- Toxicity control: the concentrations of the test article and the reference compound Aniline in the test medium were 50 and 25 mg/I, respectively, corresponding to a total actual amount of 34.8 mg DOC/l. A theoretical amount of 19.4 mg DOC/I originated from Aniline.
SAMPLING
- Sampling frequency: samples were taken on day 0 (treatment day), 7, 14, 21, 27 and 28 of the incubation period.
- Sampling method: per sampling interval, two flasks of the samples containing the test article or reference compound and one flask of the inoculum blank, the toxicity control and the abiotic control were taken and analyzed for DOC in triplicate.
- Other: water evaporation losses were compensated by adding bidistilled water at the respective sampling intervals. - Reference substance:
- aniline
- Parameter:
- % degradation (DOC removal)
- Value:
- -4 - 3
- Sampling time:
- 28 d
- Details on results:
- In the test flasks containing the test article and inoculum (test set no. 1 and 2), the mean concentrations of DOC (Dissolved Organic Carbon) varied from 32.8 to 34.7 mg/l over the exposure period of 28 days and were practically equat to the initial mean DOC concentration of 33.5 mg/l measured on day-0. Expressed as percentage DOC-removal, very low values in the range from -4 to 3 % were noted.
Therefore, the test item was found to be practically non-biodegradable under the test conditions over the 28-day exposure period to activated sludge from a domestic waste water treatment plant.
ABIOTIC CONTROL
ln the abiotic control (test set no. 6) containing the test article and sterile medium, the DOC concentrations varied from 31.9 to 34.3 mg/l over the exposure period of 28 days and were practically equalto the initial DOC concentration of 33.8 mg/l measured on day d. Therefore, no abiotic degradation occurred.
DEGRADATION IN THE TOXICITY CONTROL
ln the toxicity control (test set no. 7) containing the test article (44.3 % as DOC), the reference compound (55.7 % as DOC) and inoculum, the initial DOC concentration of 34.8 mg/l measured on day-0 decreased by 52.0 % to 16.7 mg/l within 28 days of exposure. This indicates nearly complete biodegradation of the reference compound Aniline in the presence of test article, and that the non-biodegradability of test item is not the result of a toxic effect on the microorganisms.
ln other words, 50.3 % degradation (based on DOC measured on day-0) occurred within 14 days of exposure; thus, according to the test guidelines the test article can be assumed to not be inhibitory because degradation was higher than 35 % within 14 days. - Results with reference substance:
- ln the procedure controls (test set no. 3 and 4) containing the reference compound Aniline and inoculum, Aniline was readily biodegraded by an average of 99 % within 14 days of exposure, and was fully biodegraded (102 %) by the end of the test (day 28).
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Non readily biodegradable (DOC removal (28d): -4 - 3 %)
- Executive summary:
Test item was investigated for the ready biodegradability in the "28-Day Modified OECD Screening Test". In the test flasks containing the test article and inoculum (test set no. 1 and 2) the mean concentrations of DOC (Dissolved Organic Sarbon) varied from 32.8 to 34.7 mg/l over the exposure period of 28 days and were practically equal to the initial mean DOC concentration of 33.5 mg/l measured on day-0. Expressed as percentage DOC-removal, very low values in the range from -4 to 3 % were noted over the 28-day exposure period. Therefore, test item was found to be practically nonbiodegradable in the "Modified OECD Screening Test".
In the abiotic control (test set no. 6) containing the test article and sterile medium, the DOC concentrations varied from 31.9 to 34.3 mg/l over the exposure period of 28 days and were practically equalto the initial DOC concentration of 33.8 mg/l measured on day-0. Therefore, no abiotic degradation occurred.
In the procedure controls (test set no. 3 and 4) containing the reference compound Aniline and inoculum, Aniline was readily biodegraded by an average of 99 % within 14 days of exposure, and was fully biodegraded (102 %) by the end of the test (day-28); thus confirming suitability of the activated sludge.
In the toxicity control (test set no. 7) containing the test article (44.3 % as DOC), the reference compound (55.7 % as DOC) and inoculum, the initial DOC concentration of 34.8 mg/l measured on day-0 decreased by 52.0 % to 16.7 mg/l withln 28 days of exposure. This indicates nearly complete biodegradation of the reference compound Aniline in the presence of test article, and that the non-biodegradability of test item is not the result of a toxic etfect on the microorganisms. In other words, 50.3 % degradation (based on DOC measured on day-0) occurred within 14 days of exposure; thus, according to the test guidelines the test article can be assumed to not be inhibitory because degradation was > 35 % within 14 days.
Conclusion
Non readily biodegradable (DOC removal (28d): -4 - 3 %)
Reference
mg DOC/l*, after x days | ||||||
0 | 7 | 14 | 21 | 27 | 28 | |
Blank | 0.5 | 0.7 | 0 | 0 | 0.4 | 0.3 |
Test article and inoculum | 33.4 | 35.3 | 34.3 | 33.6 | 33.3 | 31.7 |
Test article and inoculum | 34.6 | 35.4 | 33.3 | 33.2 | 33.9 | 34.5 |
Mean test article and inoculum, corrected for blank | 33.5 | 34.7 | 33.8 | 33.4 | 33.2 | 32.8 |
Aniline and inoculum | 16.9 | 12.7 | 0.1 | 0.2 | 0.5 | 0.1 |
Aniline and inoculum | 18.1 | 14.1 | 0.3 | 0.0 | 0.5 | -0.1 |
Mean aniline and inoculum, corrected for blank | 17.0 | 12.7 | 0.2 | 0.1 | 0.1 | -0.3 |
Sterile mineral nutrient solution with test article | 33.8 | 34.3 | 32.1 | 33.1 | 31.9 | 32.4 |
Test article (50 mg/l), Aniline (25 mg/l) and inoculum | 35.3 | 18.0 | 17.3 | 17.7 | 18.4 | 17 |
Test article, Aniline and inoculum, corrected for blank | 34.8 | 17.3 | 17.3 | 17.7 | 18.0 | 16.7 |
* Values given represent mean of three determinations.
Description of key information
Non readily biodegradable (DOC removal (28d): -4 - 3 %)
Key value for chemical safety assessment
Additional information
There is no information about the biodegradability potential of Reactive Yellow 215, thus the available information on the structural analogous Similar Substance 01 have been taken into consideration; the read across approach can be considered as appropriate and suitable to assess the property under investigation (details about the approach are reported into the IUCLID section 13).
Similar Substance 01 was investigated for the ready biodegradability in the "28-Day Modified OECD Screening Test". In the test flasks containing the test article and inoculum (test set no. 1 and 2) the mean concentrations of DOC (Dissolved Organic Sarbon) varied from 32.8 to 34.7 mg/l over the exposure period of 28 days and were practically equal to the initial mean DOC concentration of 33.5 mg/l measured on day-0. Expressed as percentage DOC-removal, very low values in the range from -4 to 3 % were noted over the 28-day exposure period. Therefore, test item was found to be practically nonbiodegradable in the "Modified OECD Screening Test".
In the abiotic control (test set no. 6) containing the test article and sterile medium, the DOC concentrations varied from 31.9 to 34.3 mg/l over the exposure period of 28 days and were practically equalto the initial DOC concentration of 33.8 mg/l measured on day-0. Therefore, no abiotic degradation occurred.
In the procedure controls (test set no. 3 and 4) containing the reference compound Aniline and inoculum, Aniline was readily biodegraded by an average of 99 % within 14 days of exposure, and was fully biodegraded (102 %) by the end of the test (day-28); thus confirming suitability of the activated sludge.
In the toxicity control (test set no. 7) containing the test article (44.3 % as DOC), the reference compound (55.7 % as DOC) and inoculum, the initial DOC concentration of 34.8 mg/l measured on day-0 decreased by 52.0 % to 16.7 mg/l withln 28 days of exposure. This indicates nearly complete biodegradation of the reference compound Aniline in the presence of test article, and that the non-biodegradability of test item is not the result of a toxic etfect on the microorganisms. In other words, 50.3 % degradation (based on DOC measured on day-0) occurred within 14 days of exposure; thus, according to the test guidelines the test article can be assumed to not be inhibitory because degradation was > 35 % within 14 days.
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