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Physical & Chemical properties

Water solubility

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Reference
Endpoint:
water solubility
Type of information:
experimental study
Adequacy of study:
key study
Study period:
14 February 2017 to 22 February 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 105 (Water Solubility)
Version / remarks:
1995
Deviations:
no
GLP compliance:
yes
Type of method:
flask method
Key result
Water solubility:
0.34 g/L
Conc. based on:
test mat.
Incubation duration:
5 d
Temp.:
20 °C
pH:
3
Details on results:
DEFINITIVE TEST RESULTS
Samples were taken from the three flasks at two time points: Day 4 and Day 5. Table 1 summarises the analytical measurements from the experiment. The between day difference between Day 4 and 5 was less than 15 %. No other sampling was required. The amount of test material dissolved in water was 0.34 g/L. The test temperature ranged from 19.6 to 20.3 °C (20.0 ± 0.35 °C).

OBSERVATIONS
No chemical instability was observed during the test. It was noted during method development that increasing amounts of un-dissolved test material in water led to increasing apparent solubility of the test material. The test material is a UVCB; therefore the increasing amount of material found in the water during method development may be due to preferentially soluble components in the test material or impurities.

Table 1: Results summary

Sample

Day 4 (g/L)

 

pH

Day 5 (g/L)

 

pH

Sample 1

0.33

3

0.34

3

Sample 2

0.34

3

0.36

3

Sample 3

0.34

3

0.35

3

Time point’s average

test material concentration

0.34

 

0.35

 

Within day RSD (%)

1.7

2.8

Between day average

test material concentration (g/L)

0.34

Between day difference (%)

2.6

RSD = Relative Standard Deviation

Conclusions:
Under the conditions of this study the test material had a water solubility of 0.34 g/L at 20.0 °C.
Executive summary:

The water solubility of the test material was determined in accordance with the standardised guideline OECD 105 under GLP conditions using the flask method.

A preliminary test was conducted in order to approximate the amount of test material that would dissolve in water and the time required to reach saturation. There was un-dissolved test material visible on the surface of the water in the preliminary test flask when observed after shaking overnight. Therefore the preliminary test indicated a water solubility of less than 1 g/L.

The flask method was used for determination of solubility. Based on the preliminary test results, three containers with lids were prepared containing 0.1 g of the test material in 100 mL of water. Flasks were placed in a water bath at 20.0 ± 0.5 °C with constant orbital shaking at 150 rpm until equilibrium was reached. Equilibrium was determined by either the observation that no un-dissolved test material remained, or, by two consecutive daily measurements of the dissolved test material being within 15 % of each other. An aliquot from each flask was collected after Day 4 and 5 for analysis. The between day difference between Day 4 and 5 was less than 15 % so no other sampling was required.

Under the conditions of this study the test material had a water solubility of 0.34 g/L at 20.0 °C.

Description of key information

Under the conditions of this study the test material had a water solubility of 0.34 g/L at 20.0 °C.

Key value for chemical safety assessment

Water solubility:
0.34 g/L
at the temperature of:
20 °C

Additional information

The water solubility of the test material was determined in accordance with the standardised guideline OECD 105 under GLP conditions using the flask method. The study was awarded a reliability score of 1 in accordance with the criteria set forth by Klimisch et al. (1997).

A preliminary test was conducted in order to approximate the amount of test material that would dissolve in water and the time required to reach saturation. There was un-dissolved test material visible on the surface of the water in the preliminary test flask when observed after shaking overnight. Therefore the preliminary test indicated a water solubility of less than 1 g/L.

The flask method was used for determination of solubility. Based on the preliminary test results, three containers with lids were prepared containing 0.1 g of the test material in 100 mL of water. Flasks were placed in a water bath at 20.0 ± 0.5 °C with constant orbital shaking at 150 rpm until equilibrium was reached. Equilibrium was determined by either the observation that no un-dissolved test material remained, or, by two consecutive daily measurements of the dissolved test material being within 15 % of each other. An aliquot from each flask was collected after Day 4 and 5 for analysis. The between day difference between Day 4 and 5 was less than 15 % so no other sampling was required.

Under the conditions of this study the test material had a water solubility of 0.34 g/L at 20.0 °C.