Octadeca-dienoic acid (3-dimethylamino-propyl)-amide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Octadeca-dienoic acid (3-dimethylamino-propyl)-amide

Molecular formula:

C23H44N2O

IUPAC Name:

Octadeca-dienoic acid (3-dimethylamino-propyl)-amide

Test material form:

liquid: viscous

Sampling and analysis

Analytical monitoring:

yes

Remarks:

DOC at test start

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Origin: All fish used in the test originated from an own breeding (hatching date: December 02, 2002). Parental fish were supplied by AQUARIUM AM AEGI, Feldstr. 7, D-30159 Hannover.

Holding: Holding was performed at the test facility at 23 ± 2 °C and diffuse light (0.1-10 µmol/m2s, natural photoperiod). The water was changed at least once per week. The dissolved oxygen concentration was more than 80 % of the air saturation value. Only fish from a population with mortality < 5 % within the last 7 days before start of the study were used in the test. No disease treatments were administered throughout holding and testing.

Water: Tap water of local origin was used for holding and testing. The water was filtered on activated charcoal and aerated for at least 24 h to remove chlorine.
Nominal water parameters:
Total hardness: 10 - 250 mg CaCO3/L
pH-value: 6.0 - 8.5
The water is analysed once a year acc. to German tap water regulation.

Feeding: Food was provided 3 times per week. The amount of food was 4 % of the fish body weight per feeding day. The test fish were not fed 24 h before the test was started.

Food: Trouvit 40 I 2; MILKIVIT, D-86666 Borgheim

Number of test fish: 7 zebrafish were used per test concentration and control.

Loading, length of fish: Fish density in the tanks was less than 1 g fish per liter test solution. Average body length at the test start: 2.49 cm
Average body weight at the test start: 0.15 g

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

67 mg/L as CaCO3

Test temperature:

23 ± 2 °C

pH:

7.11 - 7.69

Dissolved oxygen:

95-99% saturation

Nominal and measured concentrations:

Test concentrations: 62.5- 125- 250- 500 - 1000 µg/L, prepared with dilution water (nominal)

Stock solution: A stock solution of 10 mg/L was prepared

Details on test conditions:

A static test was performed with one application at the test initiation.

Test duration: 96h

Test vessel: Glass-aquaria loosely covered by glass tops were used.

Test volume: The test volume was about 4 L per vessel.

Aeration: Gentle aeration was provided.

Dilution water: Same as for holding.

Replicates: One replicate per test concentration and control.

Introduction of fish: Fish were introduced randomly to individual replicates.

Feeding of test fish: The fish were not fed during the test.

Water temperature: 23 ± 2 °C

Dissolved oxygen concentration: Not less than 60 % of air saturation value.

Light intensity: 0.1- 10 µmol/m2· s

Photoperiod: A natural photoperiod occurred during the test.

Water quality parameters of pH-value and oxygen-saturation measured after 0, 24, 48, 72 and 96 h were determined to be within the acceptable limits.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

354 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

250 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

500 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

250 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Details on results:

After 24, 48, 72 and 96 h mortality and behaviour of the test fish was observed.

The test item was clearly dissolved throughout the test.

No symptoms of toxicity were seen up to 250 µg/l. At 500 µg/l one fish showed lethargy before mortality occured.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In this study the test substance was found to be toxic to zebrafish after 96 h at the nominal concentration level of 500 µg/L and higher.
The LC50 (96 h) was 354 µg/L (330- 379 µg/L), the NOEC after 96 h was the test item concentration 250 µg/L (all nominal concentrations).

Executive summary:

The acute toxicity of the test item to fish (zebrafish) was determined according to OECD-Guideline for Testing of Chemicals No. 203 (1992) from 2003-03-31 to 2003-04-04 at DR.U.NOACK-LABORATORIUM FOR ANGEWANDTE BIOLOGIE, 31157 Sarstedt, Germany.

A static test was conducted with the nominal concentrations 62.5 - 125 - 250 - 500 - 1000 µg/L.

Duration of the test was 96 h. 7 test organisms were exposed to each test concentration and control.

All effect values are based on the nominal concentration of the test item.

The stock solution of the substance was analytically verified via analysis of dissolved organic carbon (DOC, according to DIN guideline 38409 part 3) at test start.

The test substance was found to be toxic to zebrafish after 96 h at the nominal concentration level of 500 µg/L and higher.

The LC50 (96 h) was 354 µg/L (330- 379 µg/L), the NOEC after 96 h was the test item concentration 250 µg/L (all nominal concentrations).

Water quality parameters of pH-value and oxygen-saturation measured after 0, 24, 48, 72 and 96 h were determined to be within the acceptable limits.

The validity criteria of the guideline were met.