3-pyridylmethanol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 2008-06-05 to 2008-07-03

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

other: Methods of Testing New Chemical Substances

Version / remarks:

version adopted 2006-11-20
(Nov21,2003; No.1121002, Pharmaceutical and Food safety bureau, ministry of health, laour and welfare;Nov.13, 2003, No.2, Manufacturing industries bureau, Ministry of Economy, trade and industry; No. 031121002)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Remarks:

HPLC

Details on sampling:

Sampling at start of study (preparation of each test concentration) and at the end of exposure.

Vehicle:

no

Details on test solutions:

Stock solution: 2000 mg of test material were diluted with test water up to 1000 mL. (=> 2000mg/L, concentration 7)
The stock solution was further diluted with test water and test concentrations were prepared as 20, 43, 93, 200, 430, 930 mg/L (concentrations 1 to 6)

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: waterflea
- Strain/clone: STRAUS
- Source: National Institute for Environmental Studies
- Age: not older than 24 hours

Test type:

static

Water media type:

freshwater

Remarks:

Elendt M4 medium

Limit test:

no

Total exposure duration:

48 h

Hardness:

250 mg/L as CaCO3

Test temperature:

20 ± 1 °C

pH:

7.8 - 8.2

Dissolved oxygen:

8.5 - 8.8 mg/L

Salinity:

NA

Nominal and measured concentrations:

Nominal concentrations: 20, 43, 93, 200, 430, 930, 2000 mg/L
Measured concentrations: 20.3, 44.0, 93.7, 201.0, 432.0, 920.0, 2020.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: beaker
- Material, size, headspace, fill volume: 100 mL, glass
- Aeration:
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light / 8 hours dark
- Light intensity: 800 lux

Reference substance (positive control):

yes

Remarks:

Potassium dischromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

676 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% confidence limit: 449 - 1170 mg/L

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

20.3 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

In the most recent test (9th - 11th Jan 2008) EC50 after 48 hrs exposure of potassium dichromate was measured as 0.76 mg test item /L (CI 95% 0.67 - 0.87 mg/L).

Validity criteria fulfilled:

yes

Conclusions:

The 48 h EC50 of the test item on Daphnia magna was determined to be 676 mg/L.

Executive summary:

In the acute immobilisation test (reference 6.1.3-1) with Daphnia magna (STRAUS), the effects of the test item were determined according to Methods of Testing New Chemical Substances <Algal Growth Inhibition Test, Daphnia Acute Immobilization Test, and Fish Acute Toxicity Test> (Nov 21,2003; No.1121002, Pharmaceutical and Food safety bureau, ministry of health, laour and welfare; Nov 13, 2003, No.2, Manufacturing industries bureau, Ministry of Economy, trade and industry; No. 031121002).The study was conducted in a static system over a period of 48 hours with nominal concentrations 20, 43, 93, 200, 430, 930, 2000 mg/L. The validity criteria of the test guideline were fulfilled. The estimated EC50 value (48 hours) of the test item is 676 mg/L.

Description of key information

The 48-h EC50 of the test item on Daphnia magna was determined to be 676 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

676 mg/L

Additional information

In the acute immobilisation test (reference 6.1.3-1) with Daphnia magna (STRAUS), the effects of the test item were determined according to Methods of Testing New Chemical Substances <Algal Growth Inhibition Test, Daphnia Acute Immobilization Test, and Fish Acute Toxicity Test> (Nov 21, 2003; No.1121002, Pharmaceutical and Food safety bureau, ministry of health, laour and welfare; Nov 13, 2003, No.2, Manufacturing industries bureau, Ministry of Economy, trade and industry; No. 031121002). The study was conducted in a static system over a period of 48 hours with nominal concentrations 20, 43, 93, 200, 430, 930, 2000 mg/L. The validity criteria of the test guideline were fulfilled. The estimated EC50 value (48 hours) of the test item is 676 mg/L.