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EC number: 308-067-0 | CAS number: 97862-23-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Data is from study report
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Principles of method if other than guideline:
- Acute Inhalation Toxicity Study of 9,10-Anthracenedione, 1, 4-Diamino-N-N’-Bis (4-C7-17-Branched Alkyl phenyl) Derives in albino rat
- GLP compliance:
- no
- Test type:
- acute toxic class method
- Limit test:
- yes
Test material
- Reference substance name:
- 9,10-Anthracenedione, 1, 4-Diamino-N-N’-Bis(4-C7-17-Branched Alkyl phenyl) Derives
- Cas Number:
- 97862-23-2
- IUPAC Name:
- 9,10-Anthracenedione, 1, 4-Diamino-N-N’-Bis(4-C7-17-Branched Alkyl phenyl) Derives
- Reference substance name:
- 9,10-Anthracenedione, 1,4-diamino-, N,N'-bis(4-C7-17-branched alkylphenyl) derivs
- IUPAC Name:
- 9,10-Anthracenedione, 1,4-diamino-, N,N'-bis(4-C7-17-branched alkylphenyl) derivs
- Reference substance name:
- 9,10-Anthracenedione, 1,4-diamino-, N,N'-bis(4-C7-17-branched alkylphenyl) derivs.
- EC Number:
- 308-067-0
- EC Name:
- 9,10-Anthracenedione, 1,4-diamino-, N,N'-bis(4-C7-17-branched alkylphenyl) derivs.
- Cas Number:
- 97862-23-2
- Molecular formula:
- C48H62N2O2
- IUPAC Name:
- 9,10-Anthracenedione, 1,4-diamino-, N,N'-bis(4-C7-17-branched alkylphenyl) derivs.
- Details on test material:
- -Substance Name: 9,10-Anthracenedione, 1,4-diamino-, N,N'-bis(4-C7-17-branched alkylphenyl) derivs
-Substance type: Organic
-Physical state: Liquid
Constituent 1
Constituent 2
Constituent 3
- Specific details on test material used for the study:
- Name: 9, 10-Anthracenedione, 1, 4-Diamino-N-N’-Bis (4-C7-17-Branched Alkylphenyl) Derives
- Physical state: Dark blue liquid
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Source : Institute for Industrial Research & Toxicology
Age : 7 to 9 weeks
Sex : Male and Female
Body weight range :200 ±20g
Identification : By cage tag and corresponding colour body marking
No. of animals per dose group :10 (5 males and 5 females)
No. of dose group : Two :Group I – Limit test (5 mg/L) and Group II – Confirmatory test (5 mg/L)
Acclimatization :Twenty healthy albino rats were selected and acclimatized for standard laboratory condition for period of one week in experimental room under veterinary examination.
Randomization : After acclimatization and veterinary examination all the selected rats randomly divided into two groups of five females and five males.
Nutritional conditions :For feeding conventional Laboratory diets may be used with an unlimited supply of drinking water.
HUSBANDRY
Environmental conditions :Air conditioned rooms with 10-15 air changes per hour, temperature between 19-25 0C, relative humidity 30-60% and illumination cycle set to 12 hours artificial fluorescent light and 12 hours dark.
Accommodation :Groups of five animals of similar sex in polypropylene cages with stainless steel grill top, facilities for food and water bottle, and bedding of clean paddy husk.
Diet : Pelleted feed supplied by Pranav agro Industries Ltd., B7/6 Ramesh Nagar, Delhi, India
Water : Community tap water passed through ‘Aqua Guard on line water filter’, was kept in glass bottles, ad-libitum
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- nose only
- Vehicle:
- other: 0.1% tween 80 in Distilled water
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus:Nanotek aerosol generator
- Exposure chamber volume: 8 liters
- Method of holding animals in test chamber:For the inhalation purpose the rats were placed in polycarbonate holder tubes positioned radically around exposure chamber, so that only the snouts and nostrils of the animals were exposed to the aerosol.
- Source and rate of air: No data available
- Method of conditioning air:The chamber was maintained at a slightly negative pressure to prevent leakage of the test atmosphere from system, as well as its dilution with outside air.
- System of generating particulates/aerosols:Nanotek aerosol generator
- Method of particle size determination:
- Treatment of exhaust air: The exhaust air was decontaminated by subsequent passage through 1% NaOH solution, silica gel and activated charcoal filters.
- Temperature, humidity, pressure in air chamber: Temperature- 23.29±1.08 - 23.47±1.74, Relative humidity (%): 46.31±3.24 - 47.34±3.56
TEST ATMOSPHERE
- Brief description of analytical method used: No data available
- Samples taken from breathing zone: no
VEHICLE
- Composition of vehicle (if applicable):0.1% tween 80 in Distilled water
- Concentration of test material in vehicle (if applicable): 5 mg/L
- Justification of choice of vehicle: No data available
- Lot/batch no. (if required): No data available
- Purity: No data available
TEST ATMOSPHERE (if not tabulated)
- Particle size distribution: less than 1 micron
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): No data available
CLASS METHOD (if applicable)
- Rationale for the selection of the starting concentration:No data available - Analytical verification of test atmosphere concentrations:
- not specified
- Duration of exposure:
- 4 h
- Concentrations:
- 5 mg/L
- No. of animals per sex per dose:
- Total: 20
Group I – Limit test (5 mg/L) : 5 males and 5 females
Group II – Confirmatory test (5 mg/L): 5 males and 5 females - Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing:closely observed for any clinical signs of toxicity at various intervals such as 1 hr, 2 hrs, 4 hrs, and 6 hrs on the day of test compound aerosol exposure and later on twice a day throughout the experimentation period of 14 days.
- Necropsy of survivors performed: yes
- Other examinations performed: Mortality, clinical signs, body weight and gross pathology were examined.
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 5 mg/L air (nominal)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Remarks on result:
- other: No mortality observed
- Mortality:
- No mortality was recorded in any of the Wistar albino rats in both limit test and confirmatory test after 4 hours of exposure.
- Clinical signs:
- other: No clinical signs of intoxication at the tested aerosol concentration of 5 mg/L observed for the period of 14 days.
- Body weight:
- No significant increase or decrease in body weight on day 7th and 14th were observed when compared with day 0.
- Gross pathology:
- No gross pathological changes were observed in treated rats.
Any other information on results incl. tables
EXPOSURE ATMOSPHERE DATA
Parameters |
Group-I (5 mg/L) (limit test) |
Group-II (5 mg/L) (confirmatory test) |
Chamber temperature °C (Mean ± S.E.) |
23.29±1.08 |
23.47±1.74 |
Relative humidity (%) (Mean ± S.E.) |
46.31±3.24 |
47.34±3.56 |
Oxygen content (%) (Mean ± S.E.) |
20.21±1.16 |
21.34±1.52 |
Mean Body Weight (gm)
S.No. |
Groups |
BODY WEIGHT (gm) |
||||
DAY 0 |
DAY 7th |
% gain or loss |
DAY14th |
% gain or loss |
||
1. |
Group-I (5.0 mg/L) |
200.37 |
205.69 |
2.65 |
211.71 |
5.65 |
2. |
Group-II (5.0 mg/L) |
203.11 |
209.82 |
3.30 |
214.24 |
5.47 |
CLINICAL SIGNS AND MORTALITY
Group: I Limit test Dose: 5.0 mg/L
Parameters |
Incidence of Clinical Signs Observed after Dosing on |
Mortality |
|||||||||||||||||||
Day 0 |
DAY |
||||||||||||||||||||
Min |
Hour |
||||||||||||||||||||
30 |
1 |
2 |
4 |
6 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
Total |
% |
|
Mortality (total) |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0/10 |
0 |
Clinical Signs |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 = No clinical sign (Normal)
+ = Mild
++ = Moderate
+++ = High
++++ = Severe
CLINICAL SIGNS AND MORTALITY
Group: II Confirmatory test Dose: 5.0 mg/L
Parameters |
Incidence of Clinical Signs Observed after Dosing on |
Mortality |
|||||||||||||||||||
Day 0 |
DAY |
||||||||||||||||||||
Min |
Hour |
||||||||||||||||||||
30 |
1 |
2 |
4 |
6 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
Total |
% |
|
Mortality (total) |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0/10 |
0 |
Clinical Signs |
0 |
0 |
0 |
0 |
6 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 = No clinical sign (Normal)
+ = Mild
++ = Moderate
+++ = High
++++ = Severe
SUMMARY OF NECROPSY FINDING
S. No. |
Fate
|
Wistar albino rats |
|
Dose (mg/l) |
|||
5.0 (limit test) |
5.0 (confirmatory test) |
||
1 |
Terminal sacrifice |
10/10 |
10/10 |
2 |
Found Dead |
0/10 |
0/10 |
3 |
Abnormalities detected |
0/10 |
0/10 |
INDIVIDUAL ANIMAL FATE & NECROPSY FINDINGS
Group: I Limit test Dose: 5.0 mg/L
Animal ID |
Fate |
Time |
Gross Findings |
20172-1 |
TS |
Day 14 |
NAD |
20172-2 |
TS |
Day 14 |
NAD |
20172-3 |
TS |
Day 14 |
NAD |
20172-4 |
TS |
Day 14 |
NAD |
20172-5 |
TS |
Day 14 |
NAD |
20172-6 |
TS |
Day 14 |
NAD |
20172-7 |
TS |
Day 14 |
NAD |
20172-8 |
TS |
Day 14 |
NAD |
20172-9 |
TS |
Day 14 |
NAD |
20172-10 |
TS |
Day 14 |
NAD |
Day 0 is the day of exposure
TS=Terminal Sacrifice
NAD=No Abnormality Detected
FD=Found Dead
INDIVIDUAL ANIMAL FATE & NECROPSY FINDINGS
Group: II Confirmatory test Dose:5.0 mg/L
Animal ID |
Fate |
Time |
Gross Findings |
20172-11 |
TS |
Day 14 |
NAD |
20172-12 |
TS |
Day 14 |
NAD |
20172-13 |
TS |
Day 14 |
NAD |
20172-14 |
TS |
Day 14 |
NAD |
20172-15 |
TS |
Day 14 |
NAD |
20172-16 |
TS |
Day 14 |
NAD |
20172-17 |
TS |
Day 14 |
NAD |
20172-18 |
TS |
Day 14 |
NAD |
20172-19 |
TS |
Day 14 |
NAD |
20172-20 |
TS |
Day 14 |
NAD |
Day 0 is the day of exposure
TS=Terminal Sacrifice
NAD=No Abnormality Detected
FD=Found Dead
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified
- Conclusions:
- Acute lethal Concentration (LC50) of test compound was found be more than 5 mg/L. when Wistar Albino Male and Female rats were inhaled for 4 hours.
- Executive summary:
In a acute inhalation toxicity study,Wistar Albino Male and Female rats were exposed with test chemical in the concentration of 5 mg/L in a dynamic nose-only cylindrical chamber built from stainless steel and glass. The chamber had a volume of 8 liters with inner and outer chamber to minimize the fluctuation in concentration and temperature for 4 hours. No mortality was recorded in any of the Wistar albino rats in both limit test and confirmatory test after 4 hours of exposure. No clinical signs of intoxication at the tested aerosol concentration of 5 mg/L observed for the period of 14 days. No significant increase or decrease in body weight on day 7thand 14thwere observed when compared with day 0. No gross pathological changes were observed in treated rats. After 72 hrs, the result obtained from limit test was confirmed in another 10 animal of both sex at similar concentration. No effects were observed in exposed rats at 5 mg/L. Therefore, Acute lethal Concentration (LC50) of test compound was found be more than 5 mg/L. when Wistar Albino Male and Female rats were inhaled for 4 hours.
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