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EC number: 268-697-6 | CAS number: 68133-69-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Short term toxicity to fish
LC50 (48h) = 12 mg/l
Short-term toxicity to aquatic invertebrates
EC50 (48 h) = 67.9 % of a saturated solution (nominal loading 100 mg/l)
Toxicity to aquatic algae and cyanobacteria
ErC50 (72h) = 63.7 % of a saturated solution (nominal loading 100 mg/l)
Additional information
Apart from a toxicity study on microorganisms, only a summary of results of a 48 -h study on Rainbow trout was available on target substance; no details were provided. Consequently, the assessment for aquatic toxicity of target substance was mainly based on available data on Similar Substance 01. Details on the read across are reported in section 13.
Both target substance and read across substance are not ready biodegradable and have a low solubility in water. In particular, water solubility of target substance was measured by flask method and resulted to be less than 20 mg/l at 20 °C; a more appropriate method in case of low soluble substances, i.e. the column elution method, was used for Similar Substance 01, for which water solubility at 20 °C was less than 0.02 mg/l.
Short-term toxicity to fish
Target substance was tested for acute toxicity to Rainbow trout in a 48 -hour test, but no details on procedures and results were available. Test sample composition was 30 % dye, 69 % sodium lignosulphonate as dispersant, 1 % dedustant. As mortality was seen, an LC0 of 7 mg/l, an LC50 of 12 mg/l and an LC100 of 19.5 mg/l were determined.
Short-term toxicity to aquatic invertebrates
Similar Substance 01 was tested for acute toxicity to daphnia magna in a 48 -hour static test according to OECD guideline 202. Purity of test sample was ca. 83 %.
Nominal concentrations tested were 100 %, 50 %, 25 %, 12.5 %, 6.25 % of a saturated solution with nominal loading of 100 mg/l. Measured concentrations at 0-hour were below the LOD of 0.04 mg/l. After 48 h of exposure, immobile daphnids were seen only at the two highest concentrations, i.e. 5/20 at 50 % and 16/20 at 100 % of saturated solution; this led to an EC50 of 67.9 %.
As all tested concentrations were below the LOD and no definite data on the possible link between nominal loading and concentration in saturated solution was available, data as % of saturated solution were referred to the nominal loading of 100 mg/l. Therefore, EC50 (48h) = 67.9 mg/l.
Toxicity to aquatic algae and cyanobacteria
Similar Substance 01 was tested on toxicity to aquatic algae and cyanobacteria in a 72 -hour static test according to OECD guideline 201.
A saturated solution with a nominal loading of 100 mg test substance/l was prepared and undissolved particles were removed by membrane filtration. Components of dilution water were added to the saturated solution. Concentrations used in the test were 6.25 - 12.5 - 25.0 - 50.0 - 100 % of the saturated solution. Three replicates were tested for each concentration and six replicates for the control. Test media were clear throughout the test period. Analytical monitoring of test solutions was done via HPLC‑DAD at 0 h and recovery rates were found to be below LOD of 0.04 mg/l.
After 72 h, growth rate inhibition was significant at the 3 highest concentrations, i.e. 8 %, 32 % and 100 %, respectively.
Similarly, yield inhibition was significant at the 3 highest concentrations, i.e. 37 %, 85 % and 100 %, respectively.
On these bases
- growth rate: ErC50 (72h) = 63.7 % of a saturated solution (100 mg/l nominal loading).
- yield: EyC50 (72h) =29.8 % of a saturated solution (100 mg/l nominal loading).
As all tested concentrations were below the LOD and no definite data on the possible link between nominal loading and concentration in saturated solution was available, data as % of saturated solution were referred to the nominal loading of 100 mg/l. Therefore, ErC50 (72h) = 63.7 mg/l and EyC50 (72h) = 29.8 mg/l.
Toxicity to microorganisms
In a study conducted according to OECD guideline 209, target substance did not cause inhibition of respiration rate of microorganisms up to a concentration of 100 mg/l after a contact time of 30 minutes.
Justification for classification or non-classification
According to the CLP Regulation (EC 1272/2008), the threshold of classification of a substance for acute aquatic toxicity is 1 mg/l. This limit is compared with LC50 (96h) for fish, EC50 (48 h) for daphnia and EC50 (72 - 96 h) for algae or aquatic plants.
As for chronic toxicity, in case of lack of chronic data, the toxic potential is identified by appropriate combinations of acute toxicity data and lack of biodegradability. The threshold of classification is 100 mg/l.
Based on EC50 values obtained, no classification for acute toxicity was applied; based on LC50 (48h) of 12 mg/l for fish, EC50 (48h) of 67.9 mg/l for daphnids and EC50 (72h) of 63.7 mg/l for algae, the substance is classified in cat. 3 for aquatic chronic toxicity according to the CLP Regulation (EC 1272/2008).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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