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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1 February 2010 - 26 February 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Uncoated nano CaCO3
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls: The control group was maintained under identical conditions but not exposed to the test item.
- The test item did not form a solution using conventional methods such as ultrasonication and high shear mixing. Therefore, a saturated solution of the test item was prepared by stirring an excess (50 mg/L) of test item with reconstituted water for 48 hours at approximately 21 °C, then removing the undissolved test material by filtration through a pre-conditioned filter (0.2 µm) to give a saturated solution (100% v/v). - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: Laboratory culture
- Age at study initiation (mean and range, SD): Less than 24 hours old
- Method of breeding: Culture conditions ensured that reproduction was by parthenogenesis.
- Feeding during test: No
- Food type: Suspension of algae (Chlorella sp.)
- Frequency: Fed daily during culturing - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- The reconstituted water had an approximate theoretical total hardness of 250 mg/L as CaCO3.
- Test temperature:
- Control: 19-21 °C
Test vessels: 20-21 °C - pH:
- Control: 7.8-7.9
Test vessels: 7.9-8.4 - Dissolved oxygen:
- Control: 96-100% ASV (air saturation value)
Test vessels: 95-100% ASV
Control: 8.7-9.3 mg O2/L
Test vessels: 8.6-9.1 mg O2/L - Nominal and measured concentrations:
- Nominal concentration: 100% v/v saturated solution of test material
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: 250 mL glass jars
- Aeration: Not during the test. During preparation, the reconstituted water was aerated until the dissolved oxygen concentration was approximately air-saturation value.
- No. of organisms per vessel: 5 daphnids/ vessel
- No. of vessels per concentration (replicates): 4 replicates/concentration
- No. of vessels per control (replicates): 4 replicates
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water
- Conductivity: <5 µS cm-1
- Culture medium different from test medium: No
- Intervals of water quality measurement: Water temperature was recorded daily throughout the test. Dissolved oxygen concentrations and pH were recorded at the start and termination of the test.
OTHER TEST CONDITIONS
- Adjustment of pH: Only if necessary during preparation of the reconstituted water, with NaOH or HCl (to pH 7.8 ± 0.2)
- Photoperiod: 16 hours light and 8 hours darkness with 20 minute dawn and dusk transition periods
- Light intensity: No data
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Any immobilisation or adverse reactions to exposure were recorded at 24 and 48 hours after the start of exposure. The criterion of effect used was that Daphnia were considered to be immobilised if they were unable to swim for approximately 15 seconds after gentle agitation.
TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: Based on the results of the range-finding test a limit test was conducted at a concentration of 100% v/v saturated solution to confirm that at the highest attainable test concentration, no immobilisation or adverse reactions to exposure were observed.
- Range finding study
- Test concentrations: 1.0, 10 and 100% v/v saturated solution of test material
- Results used to determine the conditions for the definitive study: No immobilisation was observed at the test concentrations of 1.0 and 10% v/v saturated solution. A single immobilised daphnid was observed at the 100% v/v saturated solution test concentration after 48 h exposure. This was considered to be due to natural causes rather than a toxic effect and was considered not to be significant given that only 10% immobilisation was observed. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 other: % v/v saturated solution
- Nominal / measured:
- nominal
- Conc. based on:
- other: saturated solution of test material
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 other: % v/v saturated solution
- Nominal / measured:
- nominal
- Conc. based on:
- other: saturated solution of test material
- Basis for effect:
- other: zero immobilisation
- Details on results:
- There was no immobilisation in 20 daphnids exposed to a test concentration of 100% v/v saturated solution for a period of 48 hours.
The test preparations were observed to be clear, colourless solutions throughout the duration of the test.
The study showed that there were no toxic effects at saturation. - Results with reference substance (positive control):
- 24 h EC50: 0.84 mg/L (95% confidence limits 0.72-0.97 mg/L)
48 h EC50: 0.65 mg/L (95% confidence limits 0.58-0.72 mg/L)
48 h NOEC: 0.32 mg/L - Validity criteria fulfilled:
- yes
- Remarks:
- All validity criteria were satisfied.
- Conclusions:
- The acute toxicity of calcium carbonate (nano) to Daphnia magna has been investigated and gave a 48 h EC50 of >100% v/v saturated solution. The NOEC was 100% v/v saturated solution.
The study showed that there were no toxic effects at saturation.
Reference
Description of key information
The acute toxicity of calcium carbonate (nano) to Daphnia magna has been investigated and gave a 48 h EC50 of >100% v/v saturated solution. The NOEC was 100% v/v saturated solution. The study showed that there were no toxic effects at saturation.
Key value for chemical safety assessment
Additional information
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