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EC number: 207-988-4 | CAS number: 504-29-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 2008
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Remarks:
- No further details.
- Details on sampling:
- The concentration of the test substance in the test solution was measured at the beginning and the end of the exposure, and the average value was 97 ~ 100 % of the set concentration.
- Vehicle:
- not specified
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Common name: himedaka
- Strain: Oryzias latipes - Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 11 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 96h-EC50 of the test item in fish is 11 mg/L.
- Executive summary:
An acute fish toxicity test was conducted according to OECD 203 under GLP conditions. The test item concentration was measured at the beginning and at the end of the exposure time, and remained within 97 -100% of the nominal value. The 96h-EC50 for the test item to medaka (Oryzias latipes) was determined to be 11 mg/L, based on the average value of the measured concentration.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: JIS K0102
- Version / remarks:
- Japanese Industrial Standard K 0102 [1971] (as shown in Annex of OECD 305C)
- Deviations:
- no
- Principles of method if other than guideline:
- Median tolerance limit test (TLm) test was carried out according to the procedure of Japan Industrial Standards (Japanese Standards Committee, 1971).
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Vehicle:
- yes
- Details on test solutions:
- - Chemical substances were dissolved in water and neutralized with 0.01N NaOH or HCl, if necessary. Substances which were difficult to dissolve in water were dissolved in 1ml of ethanol, and then diluted in water.
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Common name: Himedaka (Oryzias Latipes)
- Age at study initiation: same age.
- Length: 2 cm.
- Weight: 0.2 g.
ACCLIMATION
- Acclimation period: 10 days. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- After 24 or 48 hours, lethal concentration of 50% fish was determined.
- Test temperature:
- 25ºC
- pH:
- 7
- Nominal and measured concentrations:
- Not specified.
- Details on test conditions:
- TEST SYSTEM
- Fill volume: 2 L
- No. of organisms per vessel: 10 fish per trial.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionized water.
OTHER TEST CONDITIONS
Chemical substance wa dissolved in water an neutralized with 0.01N NaOH or HCl if necessary.
EFFECT PARAMETERS MEASURED: Lethal concentration of 50% fish was determined. - Reference substance (positive control):
- not required
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 6 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 12 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Other biological observations: not specified.
- Results with reference substance (positive control):
- The death rate of fish in 0.5 ppm of mercuric chloride (as Hg) should not be more than 20%, while that in 1 ppm should be more than 20% after 24h.
- Sublethal observations / clinical signs:
Table1. TLm values and partition coefficients of the aromatic nitrogen compound: 2 -aminopyridine
Substance
TLm (mg/L)(a)
Partition coeficient(b)
24 h
48 h
2-Aminopyridine
12.0
6.0
3.00
a) Ten fish of Himedaka were used in 2 L of the solution.
b) Partition coefficient between n-octanol and water.
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The 24 and 48h-LC50 was 12.0 and 6.0 mg/L respectively with the test subtance 2-aminopyridine.
- Executive summary:
A median tolerance limit (TLm) test was conducted with the test item. The test was conducted according to the procedure of Japan Industrial Standards JIS K0102 (Japanese Standards Committee, 1971). TLm is equivalent to LC50 and it was calculated for 24 and 48 hours of exposure. The 24h-LC50 was 12.0 mg/L and the 48h-LC50 was 6.0 mg/L. Moreover, the partition coefficient between n-octanol and water was calculated and it was concluded that the larger solubility of compounds in n-octanol was, the larger was the toxicity to fish.
Referenceopen allclose all
Description of key information
Weight of evidence: A study according to OECD 203 (GLP study) reports a 96h-EC50 = 11 mg/L. A study according to Japan industrial Standards (JIS-K0102, 1971), reports a 48h-EC50 = 6.0 mg/L. Based on the available data, the 96h-EC50 for freswhater fish is 11 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 11 mg/L
Additional information
Weight of evidence. One study performed according to OECD 203 (GLP study), without detailed documentation, reports the 96h-EC50 of the test item in freshwater fish Himedaka (Oryzias latipes) as 11 mg/L, based on the average value of the measured concentrations (measured at the start and at the end of expoure). Another study performed according to Japan industrial Standards (JIS-K0102, 1971), reports the 48h-EC50 of the test item in Himedaka (Oryzias latipes) as 6.0 mg/L. According to OECD 203, 96 hour exposure is the preferred data. Based on the available data, the 96h-EC50 for freswhater fish is 11 mg/L.
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