Reaction mass of 1-chlorodecane and 1-chlorotetraceane

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995-11-24 to 1996-01-17

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1995

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: 92/69/EWG

Deviations:

no

Principles of method if other than guideline:

Method: other: Directive 92/69/EEC, C.4.-C

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-chlorotetradecane

EC Number:

219-368-0

EC Name:

1-chlorotetradecane

Cas Number:

2425-54-9

Molecular formula:

C14H29Cl

IUPAC Name:

1-chlorotetradecane

Test material form:

liquid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge: municipal sewage treatment plant
- Preparation of inoculum for exposure: according to guidance
- Concentration of sludge: 4.3 g/L dry weight, 28.7mg/L suspended solid per test vessel.
- Initial cell/biomass concentration: 105 * 10^4 CFU/ml

Duration of test (contact time):

29 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

14.95 mg/L

Based on:

DOC

Remarks:

DOC has been calculated theoretically.

Initial test substance concentration 2

Initial conc.:

20.75 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: mineral medium
- Test temperature: mean 21.5°C (20.7 °C -22.4 °C)
- pH: 7.4-7.6
- Aeration of dilution water: yes
- Suspended solids concentration: 28.7 mg/L

TEST SYSTEM
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: aeration not further specified
- Measuring equipment: TAC analysis
- Details of trap for CO2: addition of NaOH, formation of Sodium carbonate

SAMPLING
- Sampling frequency: 9x during 29 days (on day 0, 2, 5, 9, 12, 16, 22, 28, and 29)
- Sampling method: TAC analysis

CONTROL AND BLANK SYSTEM
- Inoculum blank: medium without test substance, 2 replicates
- Toxicity control: medium with reference substance Sodium benzoat, 1 replicate

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

75

Sampling time:

28 d

Details on results:

Kinetic of test substance (in %):
= 30 after 5 day(s)
= 66 after 12 day(s)
= 69 after 16 day(s)
= 74 after 22 day(s)
= 74 after 28 day(s)

BOD5 / COD results

Results with reference substance:

92% after 28 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Interpretation of results:

readily biodegradable

Conclusions:

A biodegradation rate of 75% of the test substance after 28 days has been observed. More than 60% of the test substance were degraded within the 10-day window. Therefore, the test substance is readily biodegradable.