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EC number: 219-648-2 | CAS number: 2491-06-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Data is from publication.
Data source
Reference
- Reference Type:
- publication
- Title:
- Dimethylglycine and chemically related amines tested for mutagenicity under potential nitrosation conditions
- Author:
- A.J.W. Hoorn
- Year:
- 1 989
- Bibliographic source:
- Mutation Research, 222 (1989) 343-350
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- Evaluate mutagenicity of Dimethylglycine hydrochloride in Salmonella typhimurium strain TA100 by using Ames test.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- N,N-dimethylglycinium chloride
- EC Number:
- 219-648-2
- EC Name:
- N,N-dimethylglycinium chloride
- Cas Number:
- 2491-06-7
- Molecular formula:
- C4H9NO2.ClH
- IUPAC Name:
- 2-(dimethylamino)acetic acid hydrochloride
- Test material form:
- solid: crystalline
- Details on test material:
- - Name of test material : 2-(dimethylamino)acetic acid hydrochloride; N,N-dimethylglycinium chloride
- Molecular formula : C4H10ClNO2
- Molecular weight : 139.581 g/mol
- Smiles notation : OC(=O)CN(C)C.Cl
- InChl : 1S/C4H9NO2.ClH/c1-5(2)3-4(6)7;/h3H2,1-2H3,(H,6,7);1H
- Substance type: Organic
- Physical state: Solid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material : 2-(dimethylamino)acetic acid hydrochloride; N,N-dimethylglycinium chloride
- Molecular formula : C4H10ClNO2
- Molecular weight : 139.581 g/mol
- Smiles notation : OC(=O)CN(C)C.Cl
- InChl : 1S/C4H9NO2.ClH/c1-5(2)3-4(6)7;/h3H2,1-2H3,(H,6,7);1H
- Substance type: Organic
- Physical state: Solid
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 100
- Details on mammalian cell type (if applicable):
- Not applicable.
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- The microsomal S9 fraction that was used as the metabolic activation system was prepared from Sprague-Dawley adult male rat liver induced by Aroclor 1254.
- Test concentrations with justification for top dose:
- 0,1, 10,100, 500, 1000, 2500,5000and 10000 µg/plate
- Vehicle / solvent:
- Not specified.
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- other:
- Details on test system and experimental conditions:
- Details on test system and conditions
METHOD OF APPLICATION: Preincubation
DURATION
- Preincubation period: 45 min at 37 °C
- Exposure duration: 60 min at 25 °C
OTHER: For Mutagenicity 3 plates per concentration wee used. - Rationale for test conditions:
- Dose selection was based on toxicity range-finding studies both with and without metabolic activation.
- Evaluation criteria:
- A minimum increase in the number of revertants that exceeded twice the mean of the solvent control values and exhibiting a dose-effect relationship was considered to be mutagenic in strain TA100.
- Statistics:
- For all mutagenicity tests 3 plates per concentration were used and the means and standard deviations calculated.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Remarks on result:
- other: No mutagenic effect were observed
Applicant's summary and conclusion
- Conclusions:
- The substance Dimethylglycine hydrochloride(2491-06-7) was considered to be not mutagenic in S. typhimurium strains TA100.
- Executive summary:
Dimethylglycine hydrochloride were tested for its mutagenic potential. For this purpose the bacterial reverse mutation assay was performed according to OECD 471 guideline. The test material was exposed to S. typhimurium strains TA100.The substance was tested in with and without metabolic activation at concentration of 0,1, 10,100, 500, 1000, 2500,5000and 10000µg/plate. Sodium azide and 2-anthramine used as positive control. The substance Dimethylglycine hydrochloride were not toxic or induced increases in the number of revertants of TA100 with or without metabolic activation.Therefore, the substanceDimethylglycine hydrochloride was considered to be not mutagenic in S. typhimurium strains TA100. Hence the substance cannot be classified as gene mutant in vitro.
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