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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

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Diss Factsheets

Environmental fate & pathways

Endpoint summary

Administrative data

Description of key information

The biodegradation of Rhamnolipids (100% a.i.) was investigated over a 28-day period in a Manometric Respirometry Test according to OECD Guideline 301 F (1992) and EU method C.4-D (2008). The test medium was inoculated sludge from a sewage treatment plant mainly fed with municipal wastewater. The rate of degradation was monitored by measuring the quantity of oxygen required to maintain a constant gas volume in the respirometer flasks. The test item was tested in a concentration of 100 mg/L (ThOD = 173 mg/L) and 32 mg/L (ThOD = 55 mg/L) in duplicates.


The biodegradation was found to be at mean 84% (100 mg test item/L) and 106% (32 mg test item/L) after 28 days, and 63% (100 mg test item/L) and 92% (32 mg test item/L) within the 10 days window.


The degradation of the reference substance sodium benzoate had reached 81% within the first 14 days. The difference of extremes of replicate values of the removal of the test item at the end of the test for 100 mg/L is less than 20%. Therefore, the test can be considered as valid. No inhibitory effects of the test item were observed (more than 25% degradation occurred within 14 days) in the toxicity control.


According to the guideline, Rhamnolipids can be considered as readily biodegradable under the chosen test conditions.


Furthermore, the biodegradation of Rhamnolipids was investigated in an Anaerobic Degradation according to OECD 311 (March 2006) study. The degradation of the registered substance within 41 days after considering the inorganic carbon (IC) in the liquid phase was 107.5%.


The test item is biodegradable under anaerobic conditions.


In a study conducted according to OECD Guideline 106 soil sorption coefficients (Koc) from 781 (log Koc= 1.6) to 1505 (log Koc=4.3), depending on the soil type, were obtained. Based on these results and taking into account the classification of McCall et al, the test item mobility is low. Therefore, and with regard to the instability of the test item in aqueous environment, the desorption was not determined. The test item shows linear Freundlich adsorption with typical 1/n values between 0.7 and 1 in all soils except for LUFA 2.4. In this soil, a steady decrease in the mass balances was observed over the tested concentration range indicating that at least a slight test item degradation took place in


concurrence to the adsorption. This fact is in accordance with the values for the inverse regression constants 1/n > 1, indicating a significant nonlinear adsorption behaviour.

Additional information

The substance resulted to be readily biodegradable