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EC number: 259-033-6 | CAS number: 54229-13-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- disregarded due to major methodological deficiencies
- Study period:
- 1994-10-12 to 1994-10-19
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- significant methodological deficiencies
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Version / remarks:
- August 1989
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: sewage treatment plant Bürrig
- Sampling date: October 12, 1994
- Pretreatment: none
- Concentration of sludge: 1.0 g/L (dry solids) - Duration of test (contact time):
- 7 d
- Initial conc.:
- 100 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- none reported
- Reference substance:
- aniline
- Parameter:
- % degradation (DOC removal)
- Value:
- 63
- Sampling time:
- 3 h
- Remarks on result:
- other:
- Remarks:
- The high elimination within the first 3 h of the experiment indicates removal through adsorption of the test substance. No adsorption control included in the experiment.
- Parameter:
- % degradation (DOC removal)
- Value:
- 87
- Sampling time:
- 7 d
- Results with reference substance:
- 94 % degradation of aniline within 28 days.
- Validity criteria fulfilled:
- no
- Interpretation of results:
- other:
- Remarks:
- The high elimination within the first 3 h of the experiment indicates removal through adsorption of the test substance. No adsorption control included in the experiment.
- Conclusions:
- No conclusion on biodegradation can be made.
- Executive summary:
The inherent biodegradability of the test substance was investigated in accordance with OECD Guideline 302 B (Zahn-Wellens Test) using non-adapted domestic sludge. The initial test substance concentration was 100 mg DOC/L. Biodegradation was followed by measurement of DOC removal from the test vessels.
63 % elimination of DOC could be observed after 3 h. After 7 d 87% DOC have been removed.
The high elimination within the first 3 h of the experiment indicates removal through adsorption of the test substance. As no adsorption control was included in the experiment, no conclusions can be made on the actual elimination of the test substance through biodegradation. Therefore, the study is considered to have significant methodological deficiencies and to be not valid.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994-07-21 to 1994-08-18
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Version / remarks:
- Version 29/12/1992
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): collected from effluent of a laboratory-scale unit receiving predominantly domestic sewage
- Date of sampling: 15/07/1994
- Pre-treatment: none
- Initial inoculum concentration: 5ml/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 7.6 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Test temperature: 20-21 °C
- Continuous darkness: yes
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes
- Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 5
- Sampling time:
- 28 d
- Remarks on result:
- other: not readily biodegradable
- Parameter:
- COD
- Value:
- 1 050 mg O2/g test mat.
- Results with reference substance:
- 66% degradation of aniline within 28 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item is not readily biodegradable.
- Executive summary:
The ready biodegradability of the test item was investigated in accordance with Method EU C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test, Version 29.12.1992). The test substance was applied at an initial concentration of 7.6 mg/L. Aniline was used as positive control.
5 % test substance degradation could be observed within 28 d. The test item was proofed to be non-toxic to aquatic microorganisms at the applied concentration. The reference substance aniline was degraded by 66 % within 28 days. Thus, it can be concluded that the test item is not readily bioderadable.
Referenceopen allclose all
Table 1: Test results
Time |
Control DOC [mg/L] |
Test substance DOC [mg/L] |
Test substance – Control DOC [mg/L] |
Degradation [%] |
0 h |
- |
- |
100 |
0 |
3 h |
9 |
45 |
37 |
63 |
7 d |
10 |
23 |
13 |
87 |
Table 1: Biodegradation results with test item
Day |
Depletion of dissolved c(O2) [mg O2/L] |
Degradation [%] |
|
Test item |
Blank control |
||
7 |
0.75 |
0.45 |
4 |
14 |
0.70 |
0.35 |
4 |
21 |
1.05 |
0.35 |
9 |
28 |
0.90 |
0.50 |
5 |
Chemical oxygen demand (COD) of test item: 1050 mg O2/g
Description of key information
The test item showed 5 % degradation within 28 days under the conditions of test method EU C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test, Version 29.12.1992) and is thus considered to be not readily biodegradable.
63 % and 87 % elimination of the test substance (verified by DOC measurements) could be observed after 3h and 7 d, respectively, under the conditions of the OECD 302 B Test (Zahn-Wellens Test, Version August 1989). The high elimination within the first 3 h of the experiment indicates removal through adsorption of the test substance. As no adsorption control was included in the experiment, no conclusions can be made on the actual elimination of the test substance through biodegradation.
Based on the chemical structure of the test item, ready biodegradability is not expected.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
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