1-chloro-2-nitrobenzene

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Report date:

1975

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

yes

Remarks:

: lack of details on test substance; observation period only 24 h instead of 72 h.

Principles of method if other than guideline:

Method: According to FDA, Fed.Reg.38, No.187, 1973:

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-2-nitrobenzene

EC Number:

201-854-9

EC Name:

1-chloro-2-nitrobenzene

Cas Number:

88-73-3

Molecular formula:

C6H4ClNO2

IUPAC Name:

1-chloro-2-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): o-Nitrochlorbenzol
- Analytical purity: no data
- Impurities (identity and concentrations): no data

Test animals / tissue source

Species:

rabbit

Strain:

Himalayan

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 1,5 - 2 kg
- Diet: ERKA 8300, Robert Koch oHG, Hamm, Germany
- Water: ad libitum

Test system

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

other: undissolved test substance
Amount applied: 100 mg

Duration of treatment / exposure:

24 hour(s)

Observation period (in vivo):

24 hour(s)

Number of animals or in vitro replicates:

6

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): after 24 hours of application

SCORING SYSTEM:
Draize

TOOL USED TO ASSESS SCORE:
fluorescein

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

conjunctivae score

Basis:

mean

Remarks:

of 6 tested animals

Time point:

other: 24 hours

Score:

0

Max. score:

3

Remarks on result:

other: no readings performed at 48 and 72 h after instillation

Results 2

Irritation parameter:

cornea opacity score

Basis:

mean

Remarks:

of 6 tested animals

Time point:

other: 24 hour

Score:

0

Max. score:

4

Results 3

Irritation parameter:

iris score

Basis:

mean

Remarks:

of 6 tested animals

Time point:

other: 1, 7, 24 hour(s)

Score:

0

Max. score:

2

Results 4

Irritation parameter:

chemosis score

Basis:

mean

Remarks:

of 6 tested animals

Time point:

other: 1, 7, 24 hour(s)

Score:

0

Max. score:

4

Any other information on results incl. tables

Eye irritation: Undissolved substance

Animal No.	Part of the eye	Observation time
		1h	7h	24h
1	Cornea Iris Conjunctivae Chemosis	0 0 2 0	0 0 0 0	0 0 0 0
2	Cornea Iris Conjunctivae Chemosis	0 0 1 0	0 0 1 0	0 0 0 0
3	Cornea Iris Conjunctivae Chemosis	0 0 1 0	0 0 0 0	0 0 0 0
4	Cornea Iris Conjunctivae Chemosis	0 0 2 0	0 0 1 0	0 0 0 0
5	Cornea Iris Conjunctivae Chemosis	0 0 1 0	0 0 0 0	0 0 0 0
6	Cornea Iris Conjunctivae Chemosis	0 0 1 0	0 0 0 0	0 0 0 0

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Under these test conditions it can be assumed that no classification for 1-chloro-2-nitrobenzene is required because no irritating effects were observed after 24 hours. Criteria used for interpretation of results: EU

Executive summary:

Hoechst AG, 1975

The eye irritation potential of 1 -chloro-2 -nitrobenzene was investigated in 6 Himalyan rabbits according to the FDA guideline (federal register, Vol 38, No. 187, 1973). 100 mg undissolved test substance was applied into the conjunctival sac of one eye of each animal. The eyes were rinsed 24 hours after application of the test substance. The eye irritation reactions were examined and scored using the Draize method 1, 7 and 24h after application. After 1 hour 1 -chloro-2 -nitrobenzene induced slight conjunctival redness in all animals (4/6 score=1 and 2/6 score=2). These effects were reversible within 24 h. The mean scores at 24 h were 0.0 for corneal opacity, iritis, chemosis and conjunctivae in all animals. Therefore no classification according to EU classification criteria is required.