2-ethylhexylamine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Version / remarks:

May 1981

Deviations:

yes

Remarks:

Only one concentration tested which does not permit the determination of an LC50.

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.2 (Acute Toxicity (Inhalation))

Version / remarks:

April 1984

Deviations:

yes

Remarks:

Only one concentration tested which does not permit the determination of an LC50.

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPP 81-3 (Acute inhalation toxicity)

Version / remarks:

November 1984

Deviations:

yes

Remarks:

Only one concentration tested which does not permit the determination of an LC50.

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-ethylhexylamine

EC Number:

203-233-8

EC Name:

2-ethylhexylamine

Cas Number:

104-75-6

Molecular formula:

C8H19N

IUPAC Name:

2-ethylhexylamine

Specific details on test material used for the study:

- Lot/batch No.: IRAA 005

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: HOECHST AG
- Age at study initiation: 8-12 weeks
- Weight at study initiation: male: 194 - 204 g; female: 196 - 201 g
- Housing: 5 animals per cage
- Diet: Rattendiaet Altrornin 1324 (Altromin-GmbH, Lage/Lippe), ad libitum
- Water: tap water ad libitum
- Acclimation period: 5 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2 °C
- Humidity (%): 50 ± 20%
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

nose only

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure chamber volume: 60 L
- Temperature, humidity in air chamber: 22.5 - 23.4 °C; 19.7 - 32.8 %

Analytical verification of test atmosphere concentrations:

yes

Remarks:

FID-gaschromatography

Duration of exposure:

4 h

Concentrations:

1.548 mg/L (analytical)

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: daily
- Frequency of weighing: days 0, 7, 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LC50

Effect level:

< 1.548 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Mortality:

All rats died within 40 - 177 minutes after the start of exposure.

Clinical signs:

other: Exposed animals showed impairment of respiration, salivation, nasal discharge and periodically arising tonic spasms. Although tonic spasms were reported as clinical signs, these spasms were only seen in moribund animals that died shortly thereafter. Thus,

Body weight:

no data

Gross pathology:

At necropsy, macroscopic dark red spots were found on the lungs, and when the lungs were opened small quantities of white foam were detected.

Any other information on results incl. tables

The LC50 for 2-ethylhexylamine is below 1.548 mg/L air (4 h exposure). A precise evaluation of the LC50 was not performed.

Applicant's summary and conclusion

Interpretation of results:

Category 2 based on GHS criteria