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EC number: 681-644-5 | CAS number: 136369-04-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- The temperature in the positive control and in the main study was higher than stated in the guidelines. The last photometric measurement was after 70.5 hours instead of 72 hours. Both deviation were stated as uncritical.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- potassium 5-amino-1H-1,2,3,4-tetrazol-1-ide
- Cas Number:
- 136369-04-5
- Molecular formula:
- CH2KN5
- IUPAC Name:
- potassium 5-amino-1H-1,2,3,4-tetrazol-1-ide
- Test material form:
- solid: particulate/powder
- Details on test material:
- Batch no.: 08KI030
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- The concentrations of 5-Aminotetrazol Potassium were determined using a validated HPLC method.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Unicellular freshwater green alga.
Genus, Species: Desmodesmus subspicatus
Strain: CHODAT
Family: Chlorophyceae
Order: Chlorococcales
The culture of Desmodesmus subspicatus was obtained in Feb. 2008 by the "Collection of Alga“, Institut für Pflanzenphysiologie of Universität Göttingen. The alga are kept as stock culture on solid agar at 8 °C. From an aliquot of this stock culture, the pre-culture was prepared.
Study design
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 26°C to 31°C
- pH:
- 7.3 to 8.0
- Nominal and measured concentrations:
- Nominal: 0, 4.6, 10, 22, 46, 100, 220 and 460 mg/L
The measured concentrations showed very good correlation with the nominal concentrations. The recovery rate after 72 hours were between 99 and 101 %. The measured concentrations lay in a range of 100 % - 102 % of the nominal concentration. Therefore the nominal concentrations were used for the determination of the biological results. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (CAS# 7778-50-9)
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 460 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- The EC50s of potassium dichromate were determined in a current reference study. For the estimation of the EC50s of the positive control, the fits showed good statistical correspondence of the data with the dose-response-equation. The values were within the normal range of the laboratory as well as in correspondence with the values from the international ring test which are stated in EU Method C.3.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- For the estimation of the EC50s of the test item, the fits showed sufficient statistical correspondence of the data with the dose-response-equation. The analytical determinations of the test item showed very good correlation between nominal and measured concentrations and very good recovery rates. Therefore the test item 5-Aminotetrazol Potassium can be stated as stable under the test conditions and the determination of the results was based on the nominal concentrations. The result of the test can be considered valid.
- Executive summary:
Three experiments were performed. As the first and the second experiment were not valid, a third experiment was performed. The study was performed using seven concentrations ranging from 4.6 to 460 mg/L. The treatments were used to incubate the unicellular freshwater green alga Desmodesmus subspicatus for a period of 72 hours. The cell concentration of each replicate was determined by measuring the absorption of the cuvettes at 440 nm every 24 hours with a spectral photometer. With these measured values, the number of cells was calculated (linear correlation between cell concentration and absorption given). Then the growth rate μ, the area under the growth curve (AUC1) and the Yield2 were determined. At the start and at the end of the test, the content of test item in the test solutions was determined using HPLC. The recovery after 72 hours ranged from 99 % to 101 % of the start concentration. The correlation between nominal and measured concentration was 100% - 101 % at the beginning and 100 – 102 % at the end. Therefore, the determination of the results was based on the nominal concentrations. The EC50s of potassium dichromate were determined in a current reference test. The values lay within the normal range of the laboratory. The following results for the test item 5-Aminotetrazol Potassium were determined:
Endpoint NOEC LOEC EC50 Growth rate µ 4.6 mg/L 10 mg/L > 460 mg/L Biointegral AUC 10 mg/L 22 mg/L 42 mg/L Yield 4.6 mg/L 10 mg/L 11 mg/L
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