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EC number: 209-608-2 | CAS number: 587-98-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28-08-2017 - 01-09-2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed using standard test guidelines
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- no
- Specific details on test material used for the study:
- - Name of test material : sodium 3-[(4-anilinophenyl)diazenyl]benzenesulfonate
- Molecular formula : C18H14N3O3S.Na
- Molecular weight: 375.383 g/mol
- Smiles notation : c1(Nc2ccccc2)ccc(\N=N\c2cc(ccc2)S(=O)(=O)[O-])cc1.[Na+]
- InChl : 1S/C18H15N3O3S.Na/c22-25(23,24)18-8-4-7-17(13-18)21-20-16-11-9-15(10-12-16)19-14-5-2-1-3-6-14;/h1-13,19H,(H,22,23,24);/q;+1/p-1/b21-20+;
- Substance type: Organic
- Physical state: Solid - Analytical monitoring:
- not specified
- Vehicle:
- yes
- Details on test solutions:
- Preparation of the test Solutions : The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 500 mg of the test substance in 500 ml of potable water (passed through reverse osmosis system) with one hour continuous stirring for achieving test concentrations of 1.56mg/L,3.125mg/L,6.25mg/L,12.5mg/L,25mg/L,respectively.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
Animal species: Zebra fish(Danio rerio)
Animal breeder/ supplier Koyal aquarium,Nagpur.
Body length: average 1.8 cm
:Body weight: 0.662g (average)
The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
Duration of housing: 15 days
Photoperiod:16 hours light and 8 hours dark
Oxygen content: 7.7 (mg/l)
pH: 7.6
Water temperature: 24.1 °C
Mortality (%): 0
Diet: Standard brand feed, once daily
Acclimatization: Fishes, to be used in the study, are kept in the test water for seven days immediately before testing under following conditions:
Photoperiod: 16 hours light and 8 hours dark
Temperature range: 23.05 °C
pH range: 7.7
DO range:7.8 mg/l
Feeding: Daily once
Aeration: Continuous
Mortality (%): During last 48 hours = 0 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 152.5 mg of CaCO3
- Test temperature:
- 23°C
- pH:
- 7.2
- Dissolved oxygen:
- 7.4 mg/L
- Nominal and measured concentrations:
- 1.56mg/L,3.125mg/L,6.25mg/L,12.5mg/L,25mg/L
- Details on test conditions:
- Photoperiod: 16 hours light and 8 hours dark
Test vessels: The test vessels used were all plastic aquaria having the water capacity of 5liters.
Volume of water: 2 liters
No. of animals per vessel: 8 fishes
Test temperature: 23 °C
Adaptation: Test conditions corresponded to housing conditions
Test dates Test started at : 21-08-2017
Test Exposure date: 28-08-2017
Date of experimental termination : 01-09-2017
Withdrawal of food: 1 day before and during exposure
Aeration: Aeration in test vessels was provided 1 day before the start of experiment - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
pH and oxygen content:
Nominal
Concentration
(mg/l)
Number of
fish
pH readings after
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
7.6
7.6
7.9
7.9
8.0
8.0
8.2
8.2
1.56
4
4
7.8
7.8
7.9
7.9
8.1
8.1
8.3
8.3
3.125
4
4
7.8
7.8
7.9
7.9
8.1
8.1
8.3
8.3
6.25
4
4
7.8
7.8
8.0
8.0
8.1
8.1
8.4
8.4
12.5
4
4
7.8
7.8
8.0
8.0
8.1
8.1
8.4
8.4
25
4
4
7.9
7.9
8.0
8.0
8.1
8.1
8.4
8.4
Nominal
Concentration
(mg/l)
Number of
fish
Oxygen content (mg/L) after
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
7.0
7.0
6.9
6.9
6.8
6.8
6.6
6.6
1.56
4
4
6.9
6.9
6.8
6.8
6.6
6.6
6.5
6.5
3.125
4
4
6.9
6.9
6.7
6.7
6.6
6.6
6.5
6.5
6.25
4
4
6.9
6.9
6.7
6.7
6.6
6.6
6.5
6.5
12.5
4
4
6.9
6.9
6.7
6.7
6.6
6.6
6.5
6.5
25
4
4
6.9
6.9
6.7
6.7
6.6
6.6
6.5
6.5
Test temperature:
Nominal
Concentration
(mg/l)
Number of
fish
Temperature (°C) after
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
23.5
23.5
23.5
23.5
23.8
23.8
23.4
23.4
1.56
4
4
23.5
23.5
23.5
23.5
23.8
23.8
23.4
23.4
3.125
4
4
23.5
23.5
23.5
23.5
23.8
23.8
23.4
23.4
6.25
4
4
23.5
23.5
23.5
23.5
23.8
23.8
23.4
23.4
12.5
4
4
23.5
23.5
23.5
23.5
23.8
23.8
23.4
23.4
25
4
4
23.5
23.5
23.5
23.5
23.8
23.8
23.4
23.4
Mortality and symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
Mortality (cumulated) after
3 h
6 h
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
-
-
-
-
-
-
-
-
-
-
-
-
1.56
4
4
-
-
-
-
-
-
-
-
-
-
-
-
3.125
4
4
-
-
-
-
-
-
-
-
-
-
-
6.25
4
4
-
-
-
-
-
-
-
-
-
-
-
-
12.5
4
4
-
-
-
-
-
-
-
-
-
-
-
-
25
4
4
-
-
-
-
-
-
-
-
1
1
2
2
Visible Symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
Symptom (cumulated) after
3 h
6 h
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
1.56
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
3.125
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
6.25
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
12.5
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
25
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS3M1
NS3M1
NS2M1
NS2M1
NS2
NS2
Explanation of symptoms:
A: apathy
N: Narcotic-like state
C: Swimming near water surface
M: Slow movements compared to control
NS: No abnormal symptoms
S: Surfacing behaviour
L: Loss of equilibrium
SM: Shimming movement
H1: Hyperactivity
H2: Hypo activity
B: Bending behavior
F: Fin rot
IS: Invert Swimming
SB: Swimming behaviour
RF: Respiratory Function
P: Pigmentation
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for sodium 3-[(4-anilinophenyl)diazenyl]benzenesulfonate CAS No.587-98-4 on Zebra fish (Danio rerio) was determined to be 25 mg/L.
- Executive summary:
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for sodium 3-[(4-anilinophenyl)diazenyl]benzenesulfonate. The nominal concentration selected for the experiment were 1.56mg/L,3.125mg/L,6.25mg/L,12.5mg/L,25mg/L and Zebra fish (Danio rerio) were exposed to these concentration for 96 hours. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 was found to be 25 mg/L.
Thus, it can be concluded that the Sodium 3-(p-anilinophenylazo)benzenesulphonate is toxic to fish and can be considered as "aquatic chronic 3" under CLP regulation.
Reference
Description of key information
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for sodium 3-[(4-anilinophenyl)diazenyl]benzenesulfonate CAS No.587-98-4 on Zebra fish (Danio rerio) was determined to be 25 mg/L.
This value indicates that the Sodium 3-(p-anilinophenylazo)benzenesulphonate is toxic to fish and can be considered as "aquatic chronic 3" under CLP regulation.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 25 mg/L
Additional information
Two experimental studies were reviewed for short term fish endpoint of test substance Sodium 3-(p-anilinophenylazo)benzenesulphonate (CAS 587 -98 -4) which are summarised as follows.
In a key study, Fish Acute Toxicity test was conducted according to OECD Guideline 203 for sodium 3-[(4-anilinophenyl)diazenyl]benzenesulfonate. The nominal concentration selected for the experiment were 1.56mg/L,3.125mg/L,6.25mg/L,12.5mg/L,25mg/Land Zebra fish(Danio rerio) were exposed to these concentration for 96 hours.The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 was found to be 25 mg/L.
Supporting study obtained from abstract of Journal for Nature Conservation. 1 (2): 155-157, 1989 indicates that the Channa punctate were exposed to the Sodium 3-(p-anilinophenylazo)benzenesulphonate preferably for a period of 96 hours. 50 % Mortalities of the fish (LC50) were determined. On the basis of 50% mortality the LC50 value is determined to be 1.5 mg/l.
Thus, based on the available effect concentrations from key study which is 25 mg/l for target it can be concludedn that test substance Sodium 3-(p-anilinophenylazo)benzenesulphonate (CAS 587 -98 -4) was likely to be toxic to fish, hence it can be considered to be“aquatic chronic 3”as per the CLP regulation.
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